14 research outputs found
Hepatitis C infection and clearance: impact on atherosclerosis and cardiometabolic risk factors
Injection Drug Use Is a Risk Factor for HCV Infection in Urban Egypt
OBJECTIVE: To identify current risk factors for hepatitis C virus (HCV) transmission in Greater Cairo. DESIGN AND SETTING: A 1:1 matched case-control study was conducted comparing incident acute symptomatic hepatitis C patients in two "fever" hospitals of Greater Cairo with two control groups: household members of the cases and acute hepatitis A patients diagnosed at the same hospitals. Controls were matched on the same age and sex to cases and were all anti-HCV antibody negative. Iatrogenic, community and household exposures to HCV in the one to six months before symptoms onset for cases, and date of interview for controls, were exhaustively assessed. RESULTS: From 2002 to 2007, 94 definite acute symptomatic HCV cases and 188 controls were enrolled in the study. In multivariate analysis, intravenous injections (OR = 5.0; 95% CI = 1.2-20.2), medical stitches (OR = 4.2; 95% CI = 1.6-11.3), injection drug use (IDU) (OR = 7.9; 95% CI = 1.4-43.5), recent marriage (OR = 3.3; 95% CI = 1.1-9.9) and illiteracy (OR = 3.9; 95% CI = 1.8-8.5) were independently associated with an increased HCV risk. CONCLUSION: In urban Cairo, invasive health care procedures remain a source of HCV transmission and IDU is an emerging risk factor. Strict application of standard precautions during health care is a priority. Implementation of comprehensive infection prevention programs for IDU should be considered
Excess mortality rate associated with hepatitis C virus infection: A community-based cohort study in rural Egypt
International audienceBackground & aims: >80% of people chronically infected with hepatitis C virus (HCV) live in resource-limited countries, yet the excess mortality associated with HCV infection in these settings is poorly documented.Methods: Individuals were recruited from three villages in rural Egypt in 1997-2003 and their vital status was determined in 2008-2009. Mortality rates across the cohorts were compared according to HCV status: chronic HCV infection (anti-HCV antibody positive and HCV RNA positive), cleared HCV infection (anti-HCV antibody positive and HCV RNA negative) and never infected (anti-HCV antibody negative). Data related to cause of death was collected from a death registry in one village.Results: Among 18,111 survey participants enrolled in 1997-2003, 9.1% had chronic HCV infection, 5.5% had cleared HCV infection, and 85.4% had never been infected. After a mean time to follow-up of 8.6years, vital status was obtained for 16,282 (89.9%) participants. When compared to those who had never been infected with HCV in the same age groups, mortality rate ratios (MRR) of males with chronic HCV infection aged <35, 35-44, and 45-54years were 2.35 (95% CI 1.00-5.49), 2.87 (1.46-5.63), and 2.22 (1.29-3.81), respectively. No difference in mortality rate was seen in older males or in females. The all-cause mortality rate attributable to chronic HCV infection was 5.7% (95% CI: 1.0-10.1%), while liver-related mortality was 45.5% (11.3-66.4%).Conclusions: Use of a highly potent new antiviral agent to treat all villagers with positive HCV RNA may reduce all-cause mortality rate by up to 5% and hepatic mortality by up to 40% in rural Egypt
HCV iatrogenic and intrafamilial transmission in Greater Cairo, Egypt
International audienceObjectives To document hepatitis C virus (HCV) intrafamilial transmission and assess its relative importance in comparison to other current modes of transmission in the country with the largest HCV epidemic in the world. HCV intrafamilial transmission was defined as HCV transmission among relatives living in the same household.Design Caseâcontrol study. Cases were adult patients with acute hepatitis C diagnosed in two âfever hospitalsâ of Cairo. Controls were adult patients with acute hepatitis A diagnosed in the same two hospitals, and family members of cases. All consenting household members of cases provided blood for HCV serological and RNA testing. Homology of viral sequences (NS5b region) within households was used to ascertain HCV intrafamilial transmission. Exposures at risk for HCV during the 1â6â
months previous to onset of symptoms were assessed in all cases and controls.Results From April 2002 to June 2007, 100 cases with acute hepatitis C, and 678 controls (416 household members and 262 patients with acute hepatitis A) were recruited in the study. Factors independently associated with HCV infection and their attributable fractions (AFs) were the following: having had a catheter (OR=5.0, 95% CI=1.4 to 17.8; AF=6.7%), an intravenous perfusion (OR=5.8, 95% CI=2.5 to 13.3; AF=20.1%), stitches (OR=2.0, 95% CI=1.3 to 6.6; AF=10.7%), gum treatment (OR=3.7, 95% CI=1.1 to 11.9; AF=3.8%) and being illiterate (OR=2.4, 95% CI=1.4 to 4.4). Of the 100 cases, 18 had viraemic HCV-infected household members. Three long-married (>15â
years) couples were infected with virtually identical sequences and none of the three index patients reported any exposure at risk, suggesting HCV intra-familial transmission.Conclusion While three new HCV infections out of 100 could be linked to intra-familial transmission, parenteral iatrogenic transmission (dental care included) was accountable for 34.6% of these new infections. Thus, the relative contribution of intrafamilial transmission to HCV spread seems to be limited
Multivariate analysis showing factors independently associated with acute hepatitis C, Greater Cairo, 2002-7.
<p><b>NOTE</b>: Adjusted OR, odds ratio adjusted by all other covariates; CI, confidence interval.</p>e<p>7 participants with missing data: 6 controls with missing information on intravenous injection and one on duration of marriage.</p
Hepatitis C infection and clearance: impact on atherosclerosis and cardiometabolic risk factors
Socio-demographic characteristics of acute hepatitis C cases and controls sex- and ±1 year age-matched, Greater Cairo, 2002-7.
<p><b>NOTE</b>: Data are no. (%) of participants. OR, unadjusted odds ratio comparing cases to the two control groups combined; CI, confidence interval. NAâ=ânon applicable because of matching.</p
Community exposures and high risk habits among acute hepatitis C cases and controls sex- and ±1 year age-matched, Greater Cairo, 2002-7.
<p><b>NOTE</b>: Data are no. (%) of participants.</p><p>OR<sup>*</sup>, unadjusted odds ratio comparing cases to the two control groups combined; CI, confidence interval.</p>c<p>Neither cases nor controls were circumcised; none had ear piercing, tattoos or acupuncture.</p>d<p>Since women were not asked, they were excluded from the analysis (Nâ=â198; 66 participants per serie).</p
Pairwise NS5b HCV genetic distances among 133 subjects according to common exposure patterns.
<p>doi:10.1371/journal.pone.0086098.t002</p
A Novel Method to Identify Routes of Hepatitis C Virus Transmission
<div><p>Background</p><p>We propose a new approach based on genetic distances among viral strains to infer about risk exposures and location of transmission at population level.</p><p>Methods</p><p>We re-analysed 133 viral sequences obtained during a cross-sectional survey of 4020 subjects living in a hepatitis C virus (HCV) endemic area in 2002. A permutation test was used to analyze the correlation between matrices of genetic distances in the NS5b region of all pairwise combinations of the 133 viral strains and exposure status (jointly exposed or not) to several potential HCV risk factors.</p><p>Results</p><p>Compared to subjects who did not share the same characteristics or iatrogenic exposures, the median Kimura genetic distances of viral strains were significantly smaller between brothers and sisters (0.031 versus 0.102, P<0.001), mother and child (0.044 versus 0.102, P<0.001), father and child (0.045 versus 0.102, P<0.001), or subjects exposed to periodontal treatment (0.084 versus 0.102, Pâ=â0.02). Conversely, viral strains were more divergent between subjects exposed to blood transfusions (0.216 versus 0.102, Pâ=â0.04) or tooth filling or extraction (0.108, versus 0.097, Pâ=â0.05), suggesting acquisition of the virus outside of the village.</p><p>Conclusion</p><p>This method provided insights on where infection took place (household, village) for several socio-demographic characteristics or iatrogenic procedures, information of great relevance for targeting prevention interventions. This method may have interesting applications for virologists and epidemiologists studying transmission networks in health-care facilities or among intravenous drug users.</p></div