A study of annexin-V labeled-lymphocytes apoptosis in pediatric-onset systemic lupus erythematosus in comparison to juvenile rheumatoid arthritis

Background: In systemic lupus erythematosus (SLE), which is the prototype of autoimmune diseases, the autoimmune process seems to be antigen driven. Apoptosis is responsible for eliminating cells from the immune system that are autoreactive, and defects in apoptosis may contribute to autoimmune diseases such as SLE and juvenile rheumatoid arthritis (JRA). Objective: This work is aimed to study the apoptotic peripheral blood lymphocytes in patients with pediatric- onset SLE, to trace its correlations, if any, with the disease activity and clinical presentation, and to compare the apoptotic process to that in JRA, as an example of another rheumatologic disorder. Methods: The study was conducted on 32 patients with pediatric- onset SLE; their ages ranged between 5 and 25 years (mean + SD = 15.5 + 4.4). In addition to various laboratory investigations needed for diagnosis, assessment of different system involvement as well as disease activity, the percentage of early circulating apoptotic lymphocytes was measured by flowcytometry using Annexin –V. The results were compared to that of 20 age and sex matched clinically healthy children and adolescents as well as 10 JRA patients. Results: The percentage of circulating early apoptotic lymphocytes was significantly higher in SLE patients (mean ± SD = 7.02 ± 7.29 %) and JRA patients (mean ± SD=5.91± 6.00 %) as compared to healthy controls (mean ± SD = 1.89 ± 2.21 %; p=0.0003 and 0.023, respectively). The levels of apoptotic lymphocytes seemed higher in SLE patients than in JRA patients but the difference was statistically insignificant (p=0.58). There was no correlation between the percentage of circulating apoptotic lymphocytes and the disease activity markers (SLEDAI and ESR), different system involvement and the dose or duration of corticosteroids therapy. Conclusion: The general increase of circulating apoptotic lymphocytes seen in SLE patients may not be specific to SLE and could be seen with other autoimmune diseases. It seems that disturbance in the apoptotic process contributes more to the phenomenon of autoantigenicity rather than the prediction of the disease clinical activity or specific organ involvement.Keywords: SLE, apoptosis, annexin V, autoimmune diseases, JRA, PediatricEgypt J Pediatr Allergy Immunol 2003; 1(2): 118-2

Functional and structural analysis of predicted proteins obtained from homo sapiens' minisatellite 33.15-tagged transcript pAKT-45 variants

The spermatozoa are transcriptionally dormant entities which have been recognized to be an archive of mRNA, coding for a variety of functionally crucial cellular proteins. This significant repository of mRNA is predicted to be associated with early embryogenesis and postfertilization. The mRNA transcripts which are tagged with minisatellites have been involved in the regulation of the gene functions as well as their organization. However, very little information is available regarding the expression of the transcripts tagged with minisatellites in spermatozoa. Therefore, in order to understand the functions and the conformational behavior of the proteins expressed from these minisatellite-tagged transcripts, we have performed a detailed in silico analysis using the sequences of the transcripts. The protein predicted from KF274549 showed the functionalities similar to uncharacterized C4orf26 proteins, while that obtained from KF274557 predicted to be a metallophosphoesterase. Furthermore, the structural folds in the structure of these predicted proteins were analyzed by using the homology modeling and their conformational behaviors in the explicit water conditions were analyzed by using the techniques of Molecular Dynamics (MD) simulations. This detailed analysis will facilitate the understanding of these proteins in the spermatozoon region and can be used for uncovering other attributes of the metabolic network

GIZA 11 AND GIZA 12; TWO NEW FLAX DUAL PURPOSE TYPE VARIETIES

Sixteen flax genotypes {13 promising lines and 3 check varieties viz., Giza 8 (oil type), Sakha 1 (dual purpose type) and Sakha 3 (fiber type)} were evaluated for straw, seed, oil yields and their related traits under twelve different environments; four locations (Sakha, Etay El-Baroud, Ismailia and Giza Exp. Stations through three successive seasons (2011/12, 2012/13 and 2013/14). These materials were evaluated in a randomized complete blocks design with three replications at the twelve above-mentioned environments. The analysis of variance revealed highly significant differences among genotypes (G), environments (E) and G x E interaction for all studied traits except straw weight per plant, indicating a wide range of variation among genotypes, environments and these genotypes exhibited differential response to environmental conditions. The significant variance due to residual for all characters except both straw weight per plant and oil yield per fad indicated that genotypes differed with respect to their stability suggesting that prediction would be difficult, which means that mean performance alone would not be appropriate. Interaction component of variance (σ2ge) was less than the genotypic variance (σ2g) for all characters, indicating that genotypes differ in their genetic potential for these traits. This was reflected in high heritability and low discrepancy between phenotypic (PCV) and genotypic (GCV) coefficients of variability values for these traits indicating the possibility of using each of long fiber percentage, plant height and technical stem length as selection indices for improving straw weight per plant, as well as, using 1000-seed weight and capsules number per plant as selection indices for improving seed weight per plant. Yield stability (YSi) statistic indicated that S.541-C/3 and S.541-D/10 gave high mean performance and stability for straw, fiber, seed and oil yields per fad in addition to oil percentage, capsules number per plant and 1000-seed weight. Therefore, the two genotypes well be released under the name Giza 11 and Giza 12, respectively. These newly released varieties are of dual purpose type for straw, fiber, seed and oil yield. They may replace the low yielding cultivars Giza 8, Sakha 1 and Sakha 3

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Causal relationshipsamongperception of Life Stressful Events, Self-efficacy, Coping Strategies, Stress and Life Attributions among Higher Diploma Students at Assiut College of Education

The study aimed to test a model of the relationship between perception of stressful life events and perceived self-efficacy as well as coping strategies with life attributions of higher diploma students at Assiut faculty of education by testing direct and indirect effects between perceiving stressful life events and the study variables. In addition, the study aimed to find out whether there are differences in perceived self-efficacy and coping strategies as well as attributionsbetween those with positive and negative perception of stressful life events.Also, differences in perceiving stressful life events were tested across classes of the demographic variables: gender, specialization, place of living, and marital status. The sample included 410 students. Results showed that there was a causal relationship in which perception of stressful life events was affected by perceived self-efficacy (direct effect is .631, indirect effect is .356), by coping strategies (direct effect is .676, indirect effect is .313), and life attributions (direct effect is .781, indirect effect is .211). There were significant differences in perceived self-efficacy, and coping strategies as well as the problem centered coping strategies in favor of those with positive perception of stressful life events. There were significant differences in the perception of stressful life events due to demographic variables including place of living and specialization in favor of humanities majors and those who lived in cities.

The use of satellite laser observations in studying the crustal movements

The mutual tectonic displacements of the lithospheric blocks take place within the deep fracture dividing them into hundreds and thousands kilometers long. It is possible to suggest that the reason of the accumulation of considerable local shift deformations is the change of the velocity of the tectonic motion in some or other parts of fractures as a result of different physical, chemical and mechanical processes. Nowadays, the range precision of Satellite Laser Ranging (SLR) technique reaches a few millimeters level. Therefore, the space geodesy technique becomes a very important tool in detecting and monitoring recent crustal movements. Regular repeated measurements of the baselines between some stations on different plates give the possibility to construct precise and detail models of crustal movements. In this paper, the length of four baselines between Helwan-SLR station and other four SLR stations are calculated using satellite geodetical technique

MTHFR Genetic Polymorphism As a Risk Factor in Egyptian Mothers with Down Syndrome Children

Recent reports linking Down syndrome (DS) to maternal polymorphisms at the methylenetetrahydrofolate reductase (MTHFR) gene locus have generated great interest among investigators in the field. The present study aimed at evaluation of MTHFR 677C/T and 1298A/C polymorphisms in the MTHFR gene as maternal risk factors for DS. Forty two mothers of proven DS outcomes and forty eight control mothers with normal offspring were included. Complete medical and nutritional histories for all mothers were taken with special emphasis on folate intake. Folic acid intake from food or vitamin supplements was significantly low (below the Recommended Daily Allowance) in the group of case mothers compared to control mothers. Frequencies of MTHFR 677T and MTHFR 1298C alleles were significantly higher among case mothers (32.1% and 57.1%, respectively) compared to control mothers (18.7% and 32.3%, respectively). Heterozygous and homozygous genotype frequencies of MTHFR at position 677 (CT and TT) were higher among case mothers than controls (40.5% versus 25% and 11.9% versus 6.2%, respectively) with an odds ratio of 2.34 (95% confidence interval [CI] 0.93–5.89) and 2.75 (95% CI 0.95–12.77), respectively. Interestingly, the homozygous genotype frequency (CC) at position 1298 was significantly higher in case mothers than in controls (33.3% versus 2.1% respectively) with an odds ratio of 31.5 (95% CI 3.51 to 282.33) indicating that this polymorphism may have more genetic impact than 677 polymorphism. Heterozygous genotype (AC) did not show significant difference between the two groups. We here report on the first pilot study of the possible genetic association between DS and MTHFR 1298A/C genotypes among Egyptians. Further extended studies are recommended to confirm the present work

MTHFR genetic polymorphism as a risk factor in Egyptian mothers with Down syndrome children. Dis

Abstract. Recent reports linking Down syndrome (DS) to maternal polymorphisms at the methylenetetrahydrofolate reductase (MTHFR) gene locus have generated great interest among investigators in the field. The present study aimed at evaluation of MTHFR 677C/T and 1298A/C polymorphisms in the MTHFR gene as maternal risk factors for DS. Forty two mothers of proven DS outcomes and forty eight control mothers with normal offspring were included. Complete medical and nutritional histories for all mothers were taken with special emphasis on folate intake. Folic acid intake from food or vitamin supplements was significantly low (below the Recommended Daily Allowance) in the group of case mothers compared to control mothers. Frequencies of MTHFR 677T and MTHFR 1298C alleles were significantly higher among case mothers (32.1% and 57.1%, respectively) compared to control mothers (18.7% and 32.3%, respectively). Heterozygous and homozygous genotype frequencies of MTHFR at position 677 (CT and TT) were higher among case mothers than controls (40.5% versus 25% and 11.9% versus 6.2%, respectively) with an odds ratio of 2.34 (95% confidence interval [CI] 0.93-5.89) and 2.75 (95% CI 0.95-12.77), respectively. Interestingly, the homozygous genotype frequency (CC) at position 1298 was significantly higher in case mothers than in controls (33.3% versus 2.1% respectively) with an odds ratio of 31.5 (95% CI 3.51 to 282.33) indicating that this polymorphism may have more genetic impact than 677 polymorphism. Heterozygous genotype (AC) did not show significant difference between the two groups. We here report on the first pilot study of the possible genetic association between DS and MTHFR 1298A/C genotypes among Egyptians. Further extended studies are recommended to confirm the present work

Environmentally friendly radiation EDTA modified hydrogel based on gelatin for adsorptive removal of cationic and anionic dye from synthetic wastewater

Colored wastewater discharge from several industries, including paper, cosmetics, leather, dying, and textile industries, is a significant environmental management issue, especially in developing countries. Copolymerization of acrylic acid (AAc) as well as gelatin (Gltn) was performed in different compositions and crosslinked utilizing gamma irradiation to form Gltn/AAc hydrogel. EDTA has modified the hydrogel to improve adsorption properties. The effect of irradiation dose, the composition of Gltn-AAc, EDTA content, and AAc content on gel percentage was studied. The swelling behavior were studied as a time function. The prepared hydrogels’ surface morphologies and structure were confirmed with FTIR, SEM, as well as XRD. Modified hydrogel used in batch adsorption was studied to remove anionic and cationic dyes, namely methyl orange (MO) and methylene blue (MB), from simulated wastewater. The parameters of adsorption, like pH, time, and initial metals concentration, were studied. The removal of MB dye by modified Gltn/AAc/EDTA hydrogel was found to be greater than that of MO dye

Metabolic Implications of Hepatitis C Virus Infection and it's Correlation to Steatohepatitis in Chronic Hepatitis C Patients with and without Type 2 Diabetes

Background Chronic hepatitis C virus (HCV) infection is not a simple viral infection; it has many metabolic and autoimmune complications. Objective To investigate the impacts of chronic HCV infection on glucose and lipid metabolism and its correlation-if any-with body mass index (BMI) and hepatosteatosis in chronic HCV patients. Patients and Methods One hundred and three (103) chronic HCV patients were involved in this study. After blood sugar testing patients were classified into three groups; Group I: 68 chronic HCV patients with type 2 diabetes. Group II: 35 chronic HCV patients without Type 2 Diabetes and Group III: 25 patients with Type 2 Diabetes as a control group. With informed written consents and approval from Ain Shams medical ethics committee, all groups were subjected to the following: full history taking, thorough clinical examination, calculation of BMI, and measurement of the waist/hip ratio were done. Assessment of fasting plasma insulin level was done by the immune-enzymatic method. Assessment of the insulin resistance state was done by Homeostatic Model Assessment (HOMA-IR). Detection of anti-HCV was done by the 3rd generation ELISA test and confirmed by qualitative polymerase chain reaction (PCR). Results Diabetic and non diabetic chronic HCV patients were found to have significantly higher fasting plasma insulin levels and insulin resistance states than the control group. This insulin resistance was not due to increased body mass index as there was a non significant difference in BMI between all the studied groups. Positive correlations were found between plasma insulin level, liver enzymes and steatohepatitis in HCV patients whether they were diabetic or not. No correlation was found between BMI and plasma insulin level in group II patients (HCV only). Conclusion Chronic HCV infection may be regarded as an independent risk factor for the development of insulin resistance and type 2 diabetes. HCV induces insulin resistance; the key step for glucose intolerance, and virus C induced steatohepatitis therefore leading to faster progression to cirrhosis. The impacts of chronic HCV infection on glucose and lipid metabolism should be recognized in clinical care centers and addressed in future studies