6,133 research outputs found
Dopamine D-3 receptors regulate GABA(A) receptor function through a phospho-dependent endocytosis mechanism in nucleus accumbens
The dopamine D-3 receptor, which is highly enriched in nucleus accumbens (NAc), has been suggested to play an important role in reinforcement and reward. To understand the potential cellular mechanism underlying D-3 receptor functions, we examined the effect of D-3 receptor activation on GABA(A) receptor (GABA(A)R)-mediated current and inhibitory synaptic transmission in medium spiny neurons of NAc. Application of PD128907 [(4aR, 10bR)-3,4a, 4,10b-tetrahydro-4-propyl-2H, 5H-[1] benzopyrano-[4,3-b]-1,4-oxazin-9-ol hydrochloride], a specific D-3 receptor agonist, caused a significant reduction of GABAAR current in acutely dissociated NAc neurons and miniature IPSC amplitude in NAc slices. This effect was blocked by dialysis with a dynamin inhibitory peptide, which prevents the clathrin/activator protein 2 (AP2)-mediated GABA(A) receptor endocytosis. In addition, the D-3 effect on GABA(A)R current was prevented by agents that manipulate protein kinase A (PKA) activity. Infusion of a peptide derived from GABA(A) beta subunits, which contains an atypical binding motif for the clathrin AP2 adaptor complex and the major PKA phosphorylation sites and binds with high affinity to AP2 only when dephosphorylated, diminished the D-3 regulation of IPSC amplitude. The phosphorylated equivalent of the peptide was without effect. Moreover, PD128907 increased GABAAR internalization and reduced the surface expression of GABA(A) receptor beta subunits in NAc slices, which was prevented by dynamin inhibitory peptide or cAMP treatment. Together, our results suggest that D-3 receptor activation suppresses the efficacy of inhibitory synaptic transmission in NAc by increasing the phospho-dependent endocytosis of GABA(A) receptors
Anode-Coupled Readout for Light Collection in Liquid Argon TPCs
This paper will discuss a new method of signal read-out from photon detectors
in ultra-large, underground liquid argon time projection chambers. In this
design, the signal from the light collection system is coupled via capacitive
plates to the TPC wire-planes. This signal is then read out using the same
cabling and electronics as the charge information. This greatly benefits light
collection: it eliminates the need for an independent readout, substantially
reducing cost; It reduces the number of cables in the vapor region of the TPC
that can produce impurities; And it cuts down on the number of feed-throughs in
the cryostat wall that can cause heat-leaks and potential points of failure. We
present experimental results that demonstrate the sensitivity of a LArTPC wire
plane to photon detector signals. We also simulate the effect of a 1 s
shaping time and a 2 MHz sampling rate on these signals in the presence of
noise, and find that a single photoelectron timing resolution of 30 ns
can be achieved.Comment: 16 pages, 15 figure
Simulations of a mortality plateau in the sexual Penna model for biological ageing
The Penna model is a strategy to simulate the genetic dynamics of
age-structured populations, in which the individuals genomes are represented by
bit-strings. It provides a simple metaphor for the evolutionary process in
terms of the mutation accumulation theory. In its original version, an
individual dies due to inherited diseases when its current number of
accumulated mutations, n, reaches a threshold value, T. Since the number of
accumulated diseases increases with age, the probability to die is zero for
very young ages (n = T). Here, instead
of using a step function to determine the genetic death age, we test several
other functions that may or may not slightly increase the death probability at
young ages (n < T), but that decreases this probability at old ones. Our
purpose is to study the oldest old effect, that is, a plateau in the mortality
curves at advanced ages. Imposing certain conditions, it has been possible to
obtain a clear plateau using the Penna model. However, a more realistic one
appears when a modified version, that keeps the population size fixed without
fluctuations, is used. We also find a relation between the birth rate, the
age-structure of the population and the death probability.Comment: submitted to Phys. Rev.
End of Life: A Family Narrative
This paper is based on ethnographic research that examines family reaction to an elderly husband and father's end of life. From a group of 30 families in our study (family defined as a widow aged 70 and over and two adult biological children between the ages of 40 and 60), we offer an extreme case example of family bereavement. We report our findings through the open-ended responses of a widow and two children who were interviewed ten months after the death of the husband and father. Three general themes emerged: (1) how the family imputes meaning to the end of life, (2) changes in the roles of family members, and (3) the family's ways of coping with the death, particularly through their belief system. A key finding is that the meaning family members find in their loved one's death is tied to the context of his death (how and where he died), their perception of his quality of life as a whole, and their philosophical, religious, and spiritual beliefs about life, death, and the afterlife that are already in place
Improved TPB-coated Light Guides for Liquid Argon TPC Light Detection Systems
Scintillation light produced in liquid argon (LAr) must be shifted from 128
nm to visible wavelengths in light detection systems used for liquid argon
time-projection chambers (LArTPCs). To date, LArTPC light collection systems
have employed tetraphenyl butadiene (TPB) coatings on photomultiplier tubes
(PMTs) or plates placed in front of the PMTs. Recently, a new approach using
TPB-coated light guides was proposed. In this paper, we report on light guides
with improved attenuation lengths above 100 cm when measured in air. This is an
important step in the development of meter-scale light guides for future
LArTPCs. Improvements come from using a new acrylic-based coating,
diamond-polished cast UV transmitting acrylic bars, and a hand-dipping
technique to coat the bars. We discuss a model for connecting bar response in
air to response in liquid argon and compare this to data taken in liquid argon.
The good agreement between the prediction of the model and the measured
response in liquid argon demonstrates that characterization in air is
sufficient for quality control of bar production. This model can be used in
simulations of light guides for future experiments.Comment: 25 pages, 20 figure
Border Detection on Digitized Skin Tumor Images
A radial search technique is presented for detecting skin tumor borders in clinical dermatology images. First, it includes two rounds of radial search based on the same tumor center. The first-round search is independent, and the second-round search is knowledge-based tracking. Then a rescan with a new center is used to solve the blind-spot problem. The algorithm is tested on model images with excellent performance, and on 300 real clinical images with a satisfactory resul
Covering problems in edge- and node-weighted graphs
This paper discusses the graph covering problem in which a set of edges in an
edge- and node-weighted graph is chosen to satisfy some covering constraints
while minimizing the sum of the weights. In this problem, because of the large
integrality gap of a natural linear programming (LP) relaxation, LP rounding
algorithms based on the relaxation yield poor performance. Here we propose a
stronger LP relaxation for the graph covering problem. The proposed relaxation
is applied to designing primal-dual algorithms for two fundamental graph
covering problems: the prize-collecting edge dominating set problem and the
multicut problem in trees. Our algorithms are an exact polynomial-time
algorithm for the former problem, and a 2-approximation algorithm for the
latter problem, respectively. These results match the currently known best
results for purely edge-weighted graphs.Comment: To appear in SWAT 201
Fermi-surface induced modulation in an optimally doped YBCO superconductor
We have observed a Fermi-surface (FS) induced lattice modulation in a YBCO
superconductor with a wavevector along CuO chains, {\it i.e.} =(0,,0). The value of is twice the Fermi
wavevector () along {\bf b*} connecting nearly nested FS `ridges'.
The modulation exists only within O-vacancy-ordered islands
(characterized by = and persists well above and
below . Our results are consistent with the presence of a FS-induced
charge-density wave
The photomultiplier tube calibration system of the MicroBooNE experiment
We report on the design and construction of a LED-based fiber calibration system for large liquid argon time projection detectors. This system was developed to calibrate the optical systems of the MicroBooNE experiment. As well as detailing the materials and installation procedure, we provide technical drawings and specifications so that the system may be easily replicated in future LArTPC detectors.National Science Foundation (U.S.) (Grant PHY-1205175
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