Plasma VCANM levels in haemodialysis patients with survival <365 days and survival >365 days and in the control group. Horizontal line: median; hashed box = IQR; error bars: range of non-outlying values.

<p>Significance levels (calculated with one-way ANOVA test, multiple comparison): ** = p<0.01; **** = p<0.001.</p

Baseline clinical and biochemical characteristics of haemodialysis patients stratified by plasma VCANM quartiles (Q1, Q2, Q3, Q4).

<p>Continuous variables are given as medians (IQR).</p>a<p>Comparisons between groups were made using Kruskal-Wallis test for continuous variables and Chi-square test for categorical variables.</p>b<p>Body mass index was calculated as weight in kilograms divided by height in meters squared.</p><p>Baseline clinical and biochemical characteristics of haemodialysis patients stratified by plasma VCANM quartiles (Q1, Q2, Q3, Q4).</p

Odds ratio calculated for a cut-off of 365 days in the different VCANM quartiles.

<p>Bars: 95% CI.</p

Univariate and multivariate Cox regression showing the odds for death in haemodialysis patients.

<p>Univariate and multivariate Cox regression showing the odds for death in haemodialysis patients.</p

Plasma VCANM levels divided into quartiles in haemodialysis patients and in CKD stage two and three patients (controls).

<p>Horizontal line: median; hashed box = IQR; error bars: range of non-outlying values. Significance levels (calculated with Kruskal Wallis test and one-way ANOVA test, multiple comparison): **** = p<0.001.</p

Kaplan-Meier survival curves for death in 371 hemodialysis patients.

<p>Patients were stratified according to lowest, medium, and highest categories of the 7S domain of collagen type IV (P4NP_7S) plasma concentration (Log rank test, chi square = 6.90; P = 0.032).</p

Association of 7S domain of collagen type IV (P4NP_7S) plasma concentrations with plasma concentrations of the collagen type IV degradation product C4M.

<p>Association of 7S domain of collagen type IV (P4NP_7S) plasma concentrations with plasma concentrations of the collagen type IV degradation product C4M.</p

Univariable and multivariable Cox regression showing the odds for death in hemodialysis patients.

<p>Univariable survival analyses were performed using the proportional hazards regression model with all variable forced into the model. Multivariable-adjusted survival analyses were performed using the proportional hazards regression model with backward variable selection, using P<0.05 for variable retention.</p

Kaplan-Meier survival curves for death in hemodialysis patients with time since initiation of dialysis less than 90 days (upper panel; log rank test, chi square = 1.70; P = 0.427) and in patients with time since initiation of dialysis more than 90 days (lower panel; log rank test, chi square = 6.63; P = 0.036).

<p>Patients were stratified according to lowest, medium, and highest categories of the 7S domain of collagen type IV (P4NP_7S) plasma concentration.</p

Type III collagen turnover as function of age.

<p>Biomarkers reflecting degradation and formation of type III collagen were measured in serum from 303 healthy men and 314 healthy women aged 22–86 divided into 5-year age groups. (A) Formation of interstitial type III collagen (Pro-C3) and (B) degradation of interstitial type III collagen (C3M). Statistical significance of C3M and Pro-C3 between each age group was calculated using ANOVA comparing the mean of each group with the mean of every other group and is presented at the different age groups in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194458#pone.0194458.s001" target="_blank">S1 Table</a>. All data are shown as median and interquartile range.</p