18,905 research outputs found
Antibody enhancement of free-flow electrophoresis
Specific T cell clones and antibodies (ABs) were developed to study the efficiency of purifying closely associated T cells using Continuous Flow Electrophoresis System. Enhanced separation is accomplished by tagging cells first with ABs directed against the antigenic determinants on the cell surface and then with ABs against the Fc portion of the first AB. This second AB protrudes sufficiently beyond the cell membrane and glycocalyx to become the major overall cell surface potential determinant and thus causes a reduction of electrophoretic mobility. This project was divided into three phases. Phase one included development of specific T cell clones and separation of these specific clones. Phase two extends these principles to the separation of T cells from spleen cells and immunized lymph node cells. Phase three applies this double antibody technique to the separation of T cytotoxic cells from bone marrow
Method of detecting system function by measuring frequency response
Real-time battery impedance spectrum is acquired using a one-time record. Fast Summation Transformation (FST) is a parallel method of acquiring a real-time battery impedance spectrum using a one-time record that enables battery diagnostics. An excitation current to a battery is a sum of equal amplitude sine waves of frequencies that are octave harmonics spread over a range of interest. A sample frequency is also octave and harmonically related to all frequencies in the sum. The time profile of this signal has a duration that is a few periods of the lowest frequency. The voltage response of the battery, average deleted, is the impedance of the battery in the time domain. Since the excitation frequencies are known and octave and harmonically related, a simple algorithm, FST, processes the time record by rectifying relative to the sine and cosine of each frequency. Another algorithm yields real and imaginary components for each frequency
Observation of Enhanced Beaming from Photonic Crystal Waveguides
We report on the experimental observation of the beaming effect in photonic
crystals enhanced via surface modes. We experimentally map the spatial field
distribution of energy emitted from a subwavelength photonic crystal waveguide
into free-space, rendering with crisp clarity the diffractionless beaming of
energy. Our experimental data agree well with our numerical studies of the
beaming enhancement in photonic crystals with modulated surfaces. Without loss
of generality, we study the beaming effect in a photonic crystal scaled to
microwave frequencies and demonstrate the technological capacity to deliver
long-range, wavelength-scaled beaming of energy.Comment: 4 pages, 6 figure
MHD equilibria with incompressible flows: symmetry approach
We identify and discuss a family of azimuthally symmetric, incompressible,
magnetohydrodynamic plasma equilibria with poloidal and toroidal flows in terms
of solutions of the Generalized Grad Shafranov (GGS) equation. These solutions
are derived by exploiting the incompressibility assumption, in order to rewrite
the GGS equation in terms of a different dependent variable, and the continuous
Lie symmetry properties of the resulting equation and in particular a special
type of "weak" symmetries.Comment: Accepted for publication in Phys. Plasma
Design improvement of a pump wear ring labyrinth seal
The investigation was successful in obtaining two improved designs for the impeller wear ring seal of the liquid hydrogen turbopump of interest. A finite difference computer code was extensively used in a parametric computational study in determining a cavity configuration with high flow resistance due to turbulence dissipation. These two designs, along with that currently used, were fabricated and tested. The improved designs were denoted Type O and Type S. The measurements showed that Type O and Type S given 67 and 30 percent reduction in leakage over the current design, respectively. It was found that the number of cavities, the step height and the presence of a small stator groove are quite important design features. Also, the tooth thickness is of some significance. Finally, the tooth height and an additional large cavity cut out from the stator (upstream of the step) are of negligible importance
Structuring educational decisions using the multiple sorting task: An example focusing on international placements in nursing
Practical examples of the steps involved in the planning and execution of the multiple sorting task are frequently lacking in published reports. This article demonstrates how the multiple sorting task can be used to structure conversations with a group of health professionals planning an international placement for nursing students. Sixteen participants were drawn from diverse professional backgrounds, including academia, clinical practice, government policy, and placement administrators. Participants sorted 17 statements written on cards into categories of their choice and noted why they sorted the cards into these particular groups. Data were analysed using multidimensional scaling and qualitative perspectives. The analysis identified four key themes that detailed the participants’ views about international placements. These findings demonstrate how the multiple sorting task can be used to generate information that facilitates the examination of important facets of health care practice that universities could cover in preparing students for international placements
Identification and characterization of a homozygous deletion found in ovarian ascites by representational difference analysis
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