Primer design for type specific primer/probe pairs in species HAdV-C hexon.

<p>Primer design for type specific primer/probe pairs in species HAdV-C hexon.</p

LLOD for HAdV singleplex reactions (genome copies/reaction).

<p>LLOD for HAdV singleplex reactions (genome copies/reaction).</p

Primers for real-time PCR amplification of adenovirus. The accession numbers for the viral genome sequences are HAdV-C1 (AF534906), HAdV-C2 (AC_000007), HAdV-C5 (AC_000008), HAdV-C6 (FJ349096).

<p>Primers for real-time PCR amplification of adenovirus. The accession numbers for the viral genome sequences are HAdV-C1 (AF534906), HAdV-C2 (AC_000007), HAdV-C5 (AC_000008), HAdV-C6 (FJ349096).</p

Specificity table of each HAdV assay.

<p>a – Low levels of HAdV-C1 was detected with the HAdV-C2 primers and probes after 35 cycles.</p><p>b – Low levels of HAdV-C2 was detected with the HAdV-C5 primers and probes after 35 cycles.</p><p>c – Low levels of HAdV-C5 was detected with the HAdV-C6 primers and probes after 35 cycles.</p

Summary of Human adenovirus (HAdV) detection by real-time PCR using the LightCycler 2.0 and J.B.A.I.D.S. in cultured isolates from the CDPH.

<p>Summary of Human adenovirus (HAdV) detection by real-time PCR using the LightCycler 2.0 and J.B.A.I.D.S. in cultured isolates from the CDPH.</p

Range of viral load in NHRC throat swabs.

<p>Range of viral load in NHRC throat swabs.</p

Alignment of species HAdV-B Hexon sequences which span the HAdV-14 primers and TaqMan probe.

<p>Note that the HAdV-14a and HAdV-14p sequences are identical. The forward primer sequence is highlighted in green, the FAM/TAMRA probe is highlighted in aqua, and the reverse primer is highlighted in red. Asterisks underneath sequences highlight identity in the species.</p

Summary of Human adenovirus 14 detection by conventional and real-time PCR in clinical specimens from the NHRC.

<p>Summary of Human adenovirus 14 detection by conventional and real-time PCR in clinical specimens from the NHRC.</p

Sensitivity of Human adenovirus 14 detection by real-time J.B.A.I.D.S. PCR in a series of 10-fold dilutions of genomic HAdV-14 DNA (10² to 10⁷) per PCR reaction.

<p>(A) Amplification profile of real-time J.B.A.I.D.S. PCR. (B) Linear regression of the standard curve.</p

Summary of Human adenovirus 14 detection by conventional and real-time PCR in clinical specimens from the ADL.

<p>Summary of Human adenovirus 14 detection by conventional and real-time PCR in clinical specimens from the ADL.</p