Optical Density Of Bone Repair After Implantation Of Homogenous Demineralized Dentin Matrix In Diabetic Rabbits

This research evaluated the bone repair process after implantation of homogenous demineralized dentin matrix (HDDM) in surgical defects in the parietal bone of rabbits with alloxan-induced diabetes, using a polytetrafluorethylene (PTFe) barrier for guided bone regeneration. Thirty-six rabbits were used and divided into four groups: control (C, n = 12), diabetic (D, n = 12, left parietal bone), diabetic with PTFe (DPTFe, same 12 rabbits, right parietal bone), and diabetic with PTFe associated to HDDM (D-PTFe+HDDM, n = 12). Bone defects were created in the parietal bone of the rabbits and the experimental treatments were performed, where applicable. The rabbits were sacrificed after 15, 30, 60 and 90 days. The bone defects were examined radiographically and by optical density (ANOVA and Tukey test, p < .05). The radiographic findings showed that the D-PTFe+HDDM group presented greater radiopacity and better trabecular bone arrangement when compared to that of the C, D and D-PTFe groups. The statistical analysis showed significant differences in the optical density of the newly formed bone among the studied groups. It was possible to conclude that HDDM was biocompatible in diabetic rabbits.223275280Abreu, P.P., Morosolli, A., Araújo, M.M., Carvalho, V.A.P., Gomes, M.F., Effects of autogenous demineralized dentin matrix on dental socket wound healing process in human (2004) Braz Oral Res, 18, p. 52. , SupplCarvalho, V.A.P., Tosello, D.O., Salgado, M.A.C., Gomes, M.F., Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibules (2004) Int J Oral Maxillofac Implants, 19 (5), pp. 679-686Gomes, M.F., Anjos, M.J.S., Nogueira, T.O., Catanzaro-Guimarães, S.A., Autogenous demineralized dentin matrix for tissue engineering applications: Radiographic and histomorphometric studies (2002) Int J Oral Maxillofac Implants, 17 (4), pp. 488-497Gomes, M.F., Silva, M.J.S., Nogueira, T.O., Catanzaro-Guimarães, S.A., Histologic evaluation of the osteoinductive property of autogenous demineralized dentin matrix on surgical bone defects in rabbit skulls using human amniotic membrane for guided bone regeneration (2001) Int J Oral Maxillofac Implants, 16 (4), pp. 563-571Guyton, A.C., Hall, J.E., (2006) Insulin, Glucagon, and Diabetes mellitus, pp. 827-840. , Guyton AC, Hall JE. Textbook of Medical Physiology. 11th ed, Elsevier;Fiorellini, J.P., Nevins, M.L., Norkin, A., Weber, H.P., Karimbux, N.Y., The effect of insulin therapy on osseointegration in a diabetic rat model (1999) Clin Oral Implants Res, 10 (5), pp. 362-368Lu, H., Kraut, D., Gerstenfeld, L.C., Graves, D.T., Diabetes interferes with the bone formation by affecting the expression of transcription factors that regulate osteoblast differentiation (2003) Endocrinology, 144 (1), pp. 346-352Nevins, M.L., Karimbux, N.Y., Weber, H.P., Giannobile, W.V., Fiorellini, J.P., Wound healing around endosseous implants in experimental diabetes (1998) Int J Oral Maxillofac Implants, 13 (5), pp. 620-629Claro, F.A., Lima, J.R., Salgado, M.A.C., Gomes, M.F., Porous Polyethylene for tissue engineering applications in diabetic rats treated with calcitonin: Histomorphometric analysis (2005) Int J Oral Maxillofac Implants, 20 (2), pp. 211-219Catanzaro-Guimarães, S.A., Catanzaro Guimarães, B.P., Garcia, R.B., Alle, N., Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dogs (1986) Int J Oral Maxillofac Surg, 15 (2), pp. 160-169Bessho, K., Tagawa, T., Murata, M., Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction (1990) J Oral Maxillofac Surg, 48 (2), pp. 162-169Nakashima, M., Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix (1994) Arch Oral Biol, 39 (12), pp. 1085-1089Catanzaro-Guimarães SA. Possibility to reinforce bone repair with decalcified dentin matrix. In: Gesellschaft für orale Implantologie (ed.). Jahrbuch für Orale Implatologie. Berlin: Quintessenz1993. p. 33-