メーリング リスト ニヨル e-ラーニング : シンデンズ キョウイク ノ ジッセン

Mails of the same contents are transmitted to all the members at once, and a copy of the mailsimultaneously saved at a joint home page, when a member sends e-mail to a joint address of themailing list（ML）. Since transmission of the attachment file up to 1 MB is possible, around 20pictures, such as an electrocardiogram or an X-ray film, can be transmitted simultaneously. In amailing list, there is the strong point in which expense does not start at all, even when the numberof the members is very large.We started the electrocardiogram self-training system by ML in July, 2000. At present wehave 270 membership, and 3,653 copies of mail have been transmitted to ML in total. The numbersof the clinical cases showing interesting electrocardiographic findings presented in MLreached 360 cases.This method is applicable to the education of the image diagnosis of almost all areas in themedical field. I expect that The University of Tokushima Medical School and the TokushimaMedical Association introduce this system in order to promote the complement education of thefaculty students, and the postgraduate education of medical association members

Brugada ショウコウグン ノ トリアツカイ : Brugadaガタ シンデンズ オ ドウ カンリスルカ

Brugada et al . reported that electrocardiogaraphic finding of ST elevation and right bundle branch block in the right precordial lead are closely associated with cardiac sudden death during sleep in 1992, and these finding is an important substrate of idiopathic ventricular fibrillation, since then we became call this clinical condition Brugada syndrome. Brugada type ECG is not so rare abnormality in Japan, especially saddle-back type ST elevation is often encountered in mass examination or daily clinic, and lethal arrhythmias are occurred in these some cases. Several percentage of asymptomatic Brugada syndrome complicate sudden cardiac death. Therefore, diagnosis and evaluation of prognosis of Brugada type ECG become important issue for daily clinic. Various suggestions for Brugada syndrome and asymptomatic Brugada type ECG are proposed, and implantable cardioverter defibrillator （ICD） is only therapy for Brugada syndrome, but there is not yet an agreed opinion about management of Brugada type ECG. Appropriate prevention for sudden death is necessary since some asymptomatic cases with Brugada type ECG complicate ventricular fibrillation. In our daily clinic, we examine signal averaged ECG, 123I-MIBG myocardial scintigarphy and adoministration test of pilsicanide for the case with Brugada type ECG as a screening test, and perform foreknowledge of sudden death. In near future, the standardized screening test for asymptomatic Brugada type ECG will be established by further study

Results in Surgical Correction of Pectus Excavatum

The results in 37 patients who underwent surgical correction of pectus excavatum at the First Department of Surgery, Nagasaki University Hospital during a period from 1973 to 1984 were reported. A variety of surgical techniques were used, and satisfactory resuls were obtained in 29 patients (78%), including 24 excellent and 5 good appreciation. Topical postoperative complications occurred in 14 of the 37 patients (38%). Five patients had recurrence of the excavated deformity. Repair of the deformity using sternal turnover technique yielded satisfactory results in 24 of the 27 patients (88%). However, partial bone necrosis occurred in 2 patients, and recurrence of the deformity occurred in 2 patients, who underwent the classical type of turnover operation at their ages over 9 years old. Since 1982 subperichondrial dissection technique has been employed for free grafted sternal turnover operation, the results have been satisfactory without any major topical complication. The efficacy of total preservation of the perichondrium was emphasized

Approach for growth of high-quality and large protein crystals

Three crystallization methods, including crystallization in the presence of a semi-solid agarose gel, top-seeded solution growth (TSSG) and a large-scale hanging-drop method, have previously been presented. In this study, crystallization has been further evaluated in the presence of a semi-solid agarose gel by crystallizing additional proteins. A novel crystallization method combining TSSG and the large-scale hanging-drop method has also been developed

Concurrence of Recurrent Thymoma and Lung Cancer A and Review of Literature

A 46-year-old female was found to have concurrence of recurrent thymoma and lung cancer because of a transient blephaloptosis which appeared 14 years following removal of the primary thymoma. Primary thymoma was non-invasive, but recurrent thymoma invaded anterior chest wall. One year after the second operation, blephaloptosis recurred and the patient was diagnosed as myasthenia, occular type. Recurrence of non-invasive thymoma is rare. And that recurrent thymoma occurred simultaneously with lung cancer is not found in the literature. Occurrence of extra-thymic malignancy has no relationship with the progression of thymoma and may present in multiple organs. Elevation of serum-CEA was noted in the present case. Review of the thymomas treated in our department shows that serum-CEA may be used as an indicator of recurrence or progression of thymoma

Percutaneous coronary intervention for a healed erosion with excimer laser coronary angioplasty and drug-coated balloon angioplasty: a case report

BackgroundHealed plaque, characterized by distinct layers of organizing thrombus and collagen, is the hallmark of tissue self-repair. However, the efficacy of excimer laser coronary angioplasty (ELCA) followed by drug-coated balloon (DCB) angioplasty in patients with healed plaques is not fully understood.Case summaryA 42-year-old woman with a history of anxiety disorder was admitted to our institution with worsening chest pain and subsequently diagnosed with anterior non-ST-elevation myocardial infarction. Coronary angiography revealed severe stenosis in the proximal left anterior descending artery (LAD) despite Thrombolysis in Myocardial Infarction (TIMI) grade 3. Optical coherence tomography (OCT) showed healed plaques with partial macrophage accumulation and no fresh thrombus. Plaque disruption and thin-cap fibrous atheroma were not identified in the culprit lesions. Intravascular ultrasound (IVUS) confirmed high-intensity marginal irregular masses at the culprit site, suggesting that the thrombus was formed by plaque erosion rather than lipid plaque or necrotic tissue. With lesion modification using ELCA prior to DCB angioplasty, OCT examination of the LAD after ELCA showed a significant reduction in plaque burden and preserved lumen size. Post-percutaneous coronary intervention angiography revealed no stenosis with TIMI grade 3. A follow-up coronary computed tomography scan showed no angiographic restenosis, and the patient remained symptom-free.ConclusionsHere we describe a case in which OCT and IVUS evaluation suggested organizing thrombus due to erosion healing, and a favorable outcome was achieved with the combination of ELCA and DCB. The combination use of ELCA and DCB might be a potential strategy for acute coronary syndrome patients with organizing thrombus

Lung Preservation Using Cadaver Perfusion

Cadaver perfusion of in vivo lung storage method is evaluated with respect to time course of ischemic damage to lungs subsequently charged with providing total pulmonary function in comparison with survival and histological derangement of a lung. It, however, is limited within 2 hours due to failure of extracorporeal circulation with core cooling to 15°c by which splanchnic pooling causes reduced venous return. However, cadaver perfusion is useful to minimize an ischemic damage to a lung as a method of in vivo preservation

膀胱癌進展、浸潤におけるHGF-MET-MMP1 signalingの関与とMET阻害薬、カボザンチニブの有効性

OBJECTIVES To clarify the invasive mechanisms of muscle-invasive bladder cancer (BCa) would be useful for the determination of appropriate treatment strategies.We previously showed that hepatocyte growth factor (HGF)-MET signaling is correlated with invasiveness of BCa cells. Here, we investigated the effects of the MET inhibitor, cabozantinib (XL184), on BCa cells. METHODS We first conducted Western blot analysis to investigate MET expression in BCa cell lines. Next, we examined the effect of cabozantinib on their proliferation and invasive abilities using MTT and Matrigel invasion assays, respectively. Invasion assays were performed using the xCELLigence system. Additionally, to investigate the biological function of HGF-MET signaling, we analyzed gene expression profiles and performed real-time polymerase chain reaction analyses of 5637 cells that were cultivated with or without HGF stimulation, with or without cabozantinib. RESULTS MET was highly expressed in 4 of 5 BCa cell lines, and 5637 and T24 cells showed especially high protein expression of MET. Cabozantinib suppressed cell proliferation and invasion (cell index; mock, 1.49 vs HGF, 2.26 vs HGF + XL184, 1.47, P < .05). Gene expression profile analysis indicated that matrix metalloproteinase 1 (MMP1) was significantly elevated at the mRNA level with addition of HGF. Moreover, cabozantinib suppressed HGF-induced MMP1 expression in 5637 T24 cells. CONCLUSIONS These data indicate that cabozantinib suppressed MMP1 expression by blocking HGF-MET signaling and that HGF-MET-MMP1 signaling is involved in the invasiveness and proliferation of BCa cells. These results suggest that cabozantinib might prove useful for future treatment of muscleinvasive BCa

Exome sequencing of senescence-accelerated mice (SAM) reveals deleterious mutations in degenerative disease-causing genes

Background: Senescence-accelerated mice (SAM) are a series of mouse strains originally derived from unexpected crosses between AKR/J and unknown mice, from which phenotypically distinct senescence-prone (SAMP) and -resistant (SAMR) inbred strains were subsequently established. Although SAMP strains have been widely used for aging research focusing on their short life spans and various age-related phenotypes, such as immune dysfunction, osteoporosis, and brain atrophy, the responsible gene mutations have not yet been fully elucidated. Results: To identify mutations specific to SAMP strains, we performed whole exome sequencing of 6 SAMP and 3 SAMR strains. This analysis revealed 32,019 to 38,925 single-nucleotide variants in the coding region of each SAM strain. We detected Ogg1 p.R304W and Mbd4 p.D129N deleterious mutations in all 6 of the SAMP strains but not in the SAMR or AKR/J strains. Moreover, we extracted 31 SAMP-specific novel deleterious mutations. In all SAMP strains except SAMP8, we detected a p.R473W missense mutation in the Ldb3 gene, which has been associated with myofibrillar myopathy. In 3 SAMP strains (SAMP3, SAMP10, and SAMP11), we identified a p.R167C missense mutation in the Prx gene, in which mutations causing hereditary motor and sensory neuropathy (Dejerine-Sottas syndrome) have been identified. In SAMP6 we detected a p.S540fs frame-shift mutation in the Il4ra gene, a mutation potentially causative of ulcerative colitis and osteoporosis. Conclusions: Our data indicate that different combinations of mutations in disease-causing genes may be responsible for the various phenotypes of SAMP strains.ArticleBMC GENOMICS. 14:248 (2013)journal articl