3 research outputs found
Dynamics of a Globular Protein Adsorbed to Liposomal Nanoparticles
A solution-state
NMR method is proposed to investigate the dynamics
of proteins that undergo reversible association with nanoparticles
(NPs). We applied the recently developed dark-state exchange saturation
transfer experiment to obtain residue-level dynamic information on
a NP-adsorbed protein in the form of transverse spin relaxation rates, <i>R</i><sub>2</sub><sup>bound</sup>. Based on dynamic light scattering, fluorescence, circular dichroism,
and NMR spectroscopy data, we show that the test protein, human liver
fatty acid binding protein, interacts reversibly and peripherally
with liposomal NPs without experiencing significant structural changes.
The significant but modest saturation transfer from the bound state
observed at 14.1 and 23.5 T static magnetic fields, and the small
determined <i>R</i><sub>2</sub><sup>bound</sup> values were consistent with a largely
unrestricted global motion at the lipid surface. Amino acid residues
displaying faster spin relaxation mapped to a region that could represent
the epitope of interaction with an extended phospholipid chain constituting
the protein anchor. These results prove that atomic-resolution protein
dynamics is accessible even after association with NPs, supporting
the use of saturation transfer methods as powerful tools in bionanoscience
Soft Photopolymerizations Initiated by Dye-Sensitized Formation of NHC-Boryl Radicals under Visible Light
A procedure for the production of N-heterocyclic carbene–boryl
radicals (NHC-BH<sub>2</sub><sup>•</sup>) upon visible light
irradiation under soft conditions is presented. New acridine orange
(dye)/diphenyl disulfide/NHC–BH<sub>3</sub> and dye/sulfonium
salt/NHC–BH<sub>3</sub> three-component initiating systems
are introduced for the efficient visible light photopolymerization
of trimethylolpropane triacrylate. The new systems could be extendend
to polymerization reactions in water (hydroxyethyl acrylate and hydroxyethyl
methyl acrylate), which proceeded with strongly improved polydispersity.
The chemical mechanisms are investigated through EPR and photolysis
experiments
Solid-Phase Polarization Matrixes for Dynamic Nuclear Polarization from Homogeneously Distributed Radicals in Mesostructured Hybrid Silica Materials
Mesoporous hybrid silica–organic
materials containing homogeneously
distributed stable mono- or dinitroxide radicals covalently bound
to the silica surface were developed as polarization matrixes for
solid-state dynamic nuclear polarization (DNP) NMR experiments. For
TEMPO-containing materials impregnated with water or 1,1,2,2-tetrachloroethane,
enhancement factors of up to 36 were obtained at ∼100 K and
9.4 T without the need for a glass-forming additive. We show that
the homogeneous radical distribution and the subtle balance between
the concentration of radical in the material and the fraction of radicals
at a sufficient inter-radical distance to promote the cross-effect
are the main determinants for the DNP enhancements we obtain. The
material, as well as an analogue containing the poorly soluble biradical
bTUrea, is used as a polarizing matrix for DNP NMR experiments of
solutions containing alanine and pyruvic acid. The analyte is separated
from the polarization matrix by simple filtration