Machine learning based prediction of soil total nitrogen, organic carbon and moisture content by using VIS-NIR spectroscopy

It is widely known that the visible and near infrared (VIS-NIR) spectroscopy has the potential of estimating soil total nitrogen (TN), organic carbon (OC) and moisture content (MC) due to the direct spectral responses these properties have in the near infrared (NIR) region. However, improving the prediction accuracy requires advanced modelling techniques, particularly when measurement is planned for fresh (wet and un-processed) soil samples. The aim of this work is to compare the predictive performance of two linear multivariate and two machine learning methods for TN, OC and MC. The two multivariate methods investigated included principal component regression (PCR) and partial least squares regression (PLSR), whereas the machine learning methods included least squares support vector machines (LS-SVM), and Cubist. A mobile, fibre type, VIS-NIR spectrophotometer was utilised to collect soil spectra (305–2200 nm) in diffuse reflectance mode from 140 wet soil samples collected from one field in Germany. The results indicate that machine learning methods are capable of tackling non-linear problems in the dataset. LS-SVMs and the Cubist method out-performed the linear multivariate methods for the prediction of all three soil properties studied. LS-SVM provided the best prediction for MC (root mean square error of prediction (RMSEP) = 0.457% and residual prediction deviation (RPD) = 2.24) and OC (RMSEP = 0.062% and RPD = 2.20), whereas the Cubist method provided the best prediction for TN (RMSEP = 0.071 and RPD = 1.96)

Comparison of Deep Neural Networks in Detecting Field Grapevine Diseases Using Transfer Learning

Plants diseases constitute a substantial threat for farmers given the high economic and environmental impact of their treatment. Detecting possible pathogen threats in plants based on non-destructive remote sensing and computer vision methods offers an alternative to existing laboratory methods and leads to improved crop management. Vine is an important crop that is mainly affected by fungal diseases. In this study, photos from healthy leaves and leaves infected by a fungal disease of vine are used to create disease identification classifiers. The transfer learning technique was employed in this study and was used to train three different deep convolutional neural network (DCNN) approaches that were compared according to their classification accuracy, namely AlexNet, VGG-19, and Inception v3. The above-mentioned models were trained on the open-source PlantVillage dataset using two training approaches: feature extraction, where the weights of the base deep neural network model were frozen and only the ones on the newly added layers were updated, and fine tuning, where the weights of the base model were also updated during training. Then, the created models were validated on the PlantVillage dataset and retrained using a custom field-grown vine photo dataset. The results showed that the fine-tuning approach showed better validation and testing accuracy, for all DCNNs, compared to the feature extraction approach. As far as the results of DCNNs are concerned, the Inception v3 algorithm outperformed VGG-19 and AlexNet in almost all the cases, demonstrating a validation performance of 100% for the fine-tuned strategy on the PlantVillage dataset and an accuracy of 83.3% for the respective strategy on a custom vine disease use case dataset, while AlexNet achieved 87.5% validation and 66.7% accuracy for the respective scenarios. Regarding VGG-19, the validation performance reached 100%, with an accuracy of 76.7%

Comparison of Deep Neural Networks in Detecting Field Grapevine Diseases Using Transfer Learning

Plants diseases constitute a substantial threat for farmers given the high economic and environmental impact of their treatment. Detecting possible pathogen threats in plants based on non-destructive remote sensing and computer vision methods offers an alternative to existing laboratory methods and leads to improved crop management. Vine is an important crop that is mainly affected by fungal diseases. In this study, photos from healthy leaves and leaves infected by a fungal disease of vine are used to create disease identification classifiers. The transfer learning technique was employed in this study and was used to train three different deep convolutional neural network (DCNN) approaches that were compared according to their classification accuracy, namely AlexNet, VGG-19, and Inception v3. The above-mentioned models were trained on the open-source PlantVillage dataset using two training approaches: feature extraction, where the weights of the base deep neural network model were frozen and only the ones on the newly added layers were updated, and fine tuning, where the weights of the base model were also updated during training. Then, the created models were validated on the PlantVillage dataset and retrained using a custom field-grown vine photo dataset. The results showed that the fine-tuning approach showed better validation and testing accuracy, for all DCNNs, compared to the feature extraction approach. As far as the results of DCNNs are concerned, the Inception v3 algorithm outperformed VGG-19 and AlexNet in almost all the cases, demonstrating a validation performance of 100% for the fine-tuned strategy on the PlantVillage dataset and an accuracy of 83.3% for the respective strategy on a custom vine disease use case dataset, while AlexNet achieved 87.5% validation and 66.7% accuracy for the respective scenarios. Regarding VGG-19, the validation performance reached 100%, with an accuracy of 76.7%

Non-Destructive Early Detection and Quantitative Severity Stage Classification of Tomato Chlorosis Virus (ToCV) Infection in Young Tomato Plants Using Vis–NIR Spectroscopy

Tomato chlorosis virus (ToCV) is a serious, emerging tomato pathogen that has a significant impact on the quality and quantity of tomato production worldwide. Detecting ToCV via means of spectral measurements in an early pre-symptomatic stage offers an alternative to the existing laboratory methods, leading to better disease management in the field. In this study, leaf spectra from healthy and diseased leaves were measured with a spectrometer. The diseased leaves were subjected to RT-qPCR for the detection and quantification of the titer of ToCV. Neighborhood component analysis (NCA) algorithm was employed for the feature selection of the effective wavelengths and the most important vegetation indices out of the 24 that were tested. Two machine learning methods, namely XY-fusion network (XY-F) and multilayer perceptron with automated relevance determination (MLP–ARD), were employed for the estimation of the disease existence and viral load in the tomato leaves. The results showed that before outlier elimination, the MLP–ARD classifier generally outperformed the XY-F network with an overall accuracy of 92.1% against 88.3% for the XY-F. Outlier elimination contributed to the performance of the classifiers as the overall accuracy for both XY-F and MLP–ARD reached 100%