25 research outputs found

    Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands

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    Rapid qPCR methods for enumerating enterococci can provide results in a few hours, thereby enhancing public health protection. Analysis of 140 samples collected from 11 beaches in Hawaii for enterococci using EPA Method 1611 revealed that a majority (70%) of samples yielded unusable data using the recommended protocol due to DNA losses during extraction. The DNA loss was correlated to the presence of suspended coralline sand. Acidification of samples alleviated or removed the interference, enabling successful method application. There were significant correlations across the three methods evaluated (Enterolert®/Method 1600 R2 = 0.85, Enterolert®/ Method 1611 R2 = 0.78 and Method 1600/1611 R2 = 0.82). When samples were not compromised, there was also good agreement among methods for beach management decisions. This study presents a protocol for beach areas with coralline sands, and re-emphasizes the need to use appropriate controls to prevent underestimation of bacterial concentrations at recreational beaches

    Comparison of Condition Rating Systems for Bridges in Three European Countries

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    Europe faces many problems connected to ageing infrastructure which was built in the second half of the 20th century. Bridges are one of the crucial elements of these infrastructures. In recent years, European countries have witnessed many failures of bridges across the continent. For example, the collapse of Viadotto Polcevera in Genoa caught the attention of society regarding its tragic consequences. Therefore, engineers must deal with the assessment of existing bridges which is essential for proper decision-making. Condition rating systems for bridges vary from country to country. Consequently, these differences in the methodology can lead to different conclusions related to the future service of assessed structures. For these reasons, this paper briefly describes condition rating systems for road bridges in Italy, Slovakia, and Portugal and defines the differences in the methodology. Subsequently, the obtained conclusions are compared and discussed. The aim of the paper is to encourage standardization in the assessment of bridge health conditions within European countries, highlighting the differences in the current systems adopted by various countries
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