42,785 research outputs found
Genomic dissection of the 1994 Cronobacter sakazakii outbreak in a French neonatal intensive care unit
Background: Cronobacter sakazakii is a member of the genus Cronobacter that has frequently been isolated from powdered infant formula (PIF) and linked with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. The Cronobacter MLST scheme has reported over 400 sequence types and 42 clonal complexes; however C. sakazakii clonal complex 4 (CC4) has been linked strongly with neonatal infections, especially meningitis. There have been a number of reported Cronobacter outbreaks over the last three decades. The largest outbreak of C. sakazakii was in a neonatal intensive care unit (NICU) in France (1994) that lasted over 3 months and claimed the lives of three neonates. The present study used whole genome sequencing data of 26 isolates obtained from this outbreak to reveal their relatedness. This study is first of its kind to use whole genome sequencing data to analyse a Cronobacter outbreak. Methods: Whole genome sequencing data was generated for 26 C. sakazakii isolates on the Illumina MiSeq platform. The whole genome phylogeny was determined using Mugsy and RaxML. SNP calls were determined using SMALT and SAMtools, and filtered using VCFtools. Results: The whole genome phylogeny suggested 3 distant clusters of C. sakazakii isolates were associated with the outbreak. SNP typing and phylogeny indicate the source of the C. sakazakii could have been from extrinsic contamination of reconstituted infant formula from the NICU environment and personnel. This pool of strains would have contributed to the prolonged duration of the outbreak, which was up to 3 months. Furthermore 3 neonates were co-infected with C. sakazakii from two different genotype clusters. Conclusion: The genomic investigation revealed the outbreak consisted of an heterogeneous population of C. sakazakii isolates. The source of the outbreak was not identified, but probably was due to environmental and personnel reservoirs resulting in extrinsic contamination of the neonatal feeds. It also indicated that C. sakazakii isolates from different genotype clusters have the ability to co-infect neonates
Draft genome sequences of three newly identified species in the genus Cronobacter, C. helveticus LMG23732T, C. pulveris LMG24059, and C. zurichensis LMG23730T
Cronobacter helveticus, Cronobacter pulveris, and
Cronobacter zurichensis are newly described species in the
Cronobacter genus, which is associated with serious infections of neonates. This is the first report of draft genome sequences for these species
Draft genome sequence of the earliest Cronobacter sakazakii sequence type 4 strain, NCIMB 8272
The Cronobacter sakazakii clonal lineage defined as sequence type 4 (ST4) is associated with severe cases of neonatal meningitis and persistence in powdered infant formula. For genome sequencing of the earliest deposited culture collection strain of Cronobacter sakazakii ST4, we used the strain NCIMB 8272, originally isolated from milk powder in 1950
Draft genome sequence of "Candidatus Cronobacter colletis" NCTC 14934T, a new species in the genus Cronobacter
Members of the Cronobacter genus are associated with serious infections in neonates. This is the first report of the draft genome sequence for the newly proposed species Cronobacter colletis
Draft genome sequence of a meningitic isolate of Cronobacter sakazakii clonal complex 4, strain 8399
The Cronobacter sakazakii clonal lineage defined as clonal complex 4 (CC4), composed of nine sequence types, is associated with severe cases of neonatal meningitis. To date, only closely related C. sakazakii sequence type 4 (ST4) strains have been sequenced. C. sakazakii strain 8399, isolated from a case of neonatal meningitis, was sequenced as the first non-ST4 C. sakazakii strain
Coherent phonon scattering effects on thermal transport in thin semiconductor nanowires
The thermal conductance by phonons of a quasi-one-dimensional solid with
isotope or defect scattering is studied using the Landauer formalism for
thermal transport. The conductance shows a crossover from localized to Ohmic
behavior, just as for electrons, but the nature of this crossover is modified
by delocalization of phonons at low frequency. A scalable numerical
transfer-matrix technique is developed and applied to model
quasi-one-dimensional systems in order to confirm simple analytic predictions.
We argue that existing thermal conductivity data on semiconductor nanowires,
showing an unexpected linear dependence, can be understood through a model that
combines incoherent surface scattering for short-wavelength phonons with nearly
ballistic long-wavelength phonons. It is also found that even when strong
phonon localization effects would be observed if defects are distributed
throughout the wire, localization effects are much weaker when defects are
localized at the boundary, as in current experiments.Comment: 13 page
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