Venn diagram showing intersections of proteins differentially expressed after VOO, FVOO, and FVOOT interventions.

<p>Proteins are presented with their gene encode symbol. Red proteins: up-regulated; green proteins: down-regulated. VOO: Virgin Olive Oil; FVOO: Functional Virgin Olive Oil enriched with its own PCs; FVOOT: Functional Virgin Olive Oil enriched with its own PCs plus complementary phenols from thyme.</p

Characteristics of the study participants at baseline.

<p>Values expressed as mean ± standard deviation (SD) or median (25^th to 75^th percentile). Sequence 1 = FVOO, FVOOT and VOO; Sequence 2 = FVOOT, VOO and FVOO; Sequence 3 = VOO, FVOO and FVOOT. No significant differences between groups were observed. To compare means or medians among groups, ANOVA or Kruskal-Wallis test were performed, respectively; whereas χ2 and exact F-test, as appropriate, were computed to compare proportions. Abbreviations: SBP, systolic blood pressure; DBP, diastolic blood pressure.</p><p>Characteristics of the study participants at baseline.</p

Top scored associated network generated by IPA describing common differentially expressed proteins after all VOO interventions.

<p>The top scoring significantly associated network was assessed with Fisher’s exact test (<i>p</i><0.01). Proteins were presented with their gene encode symbol. The proteins indicated in red and green are those whose expression levels were significantly up- or down-regulated, respectively. Proteins indicated in white are those available in the IPA database, but not detected as differentially expressed in the present study. The shapes of the symbols denote the molecular class of proteins. Solid lines indicate direct molecular interactions, whereas dashed lines indicate indirect molecular interactions.</p

Expression levels of Apo A-I, Apo A-II, Clusterin and Haptoglobin.

<p>Expression levels of Apo A-I and A-II were measured by immunturbidimetry assays and clusterin and haptoglobin by ELISA assays in 10 HDL pools.</p

Experimental protocol for the intervention study.

<p>VOO: Virgin Olive Oil; FVOO: Functional Virgin Olive Oil enriched with its own PC; FVOOT: Functional Virgin Olive Oil enriched with its own PC plus complementary phenols from thyme. Blood collection for proteomic analysis: Visit 1, baseline; Visit 3, post-first intervention; Visit 5, post-second intervention; Visit 7, post-third intervention.</p

Participants Flow-chart based on post-interventions of HDL proteomics variable.

<p>Sequence 1: FVOO, FVOOT and VOO; Sequence 2: FVOOT, VOO and FVOO; Sequence 3: VOO, FVOO and FVOOT.</p

The top 7 signaling pathways that were significantly affected by the 15 common proteins differentially expressed after all VOO interventions.

<p>Significance levels were assessed with Fisher’s exact test (<i>p</i><0.01). The pathways were ranked by <i>p</i> value. Blue bars indicate the negative log value (<i>p</i>-value). The ratio was calculated as the number of molecules in a given pathway that meet cutoff criteria divided by total number of molecules that make up that pathway.</p

Identification of a new locus and validation of previously reported loci showing differential methylation associated with smoking. The REGICOR study

<p>Smoking increases the risk of many diseases and could act through changes in DNA methylation patterns. The aims of this study were to determine the association between smoking and DNA methylation throughout the genome at cytosine-phosphate-guanine (CpG) site level and genomic regions. A discovery cross-sectional epigenome-wide association study nested in the follow-up of the REGICOR cohort was designed and included 645 individuals. Blood DNA methylation was assessed using the Illumina HumanMethylation450 BeadChip. Smoking status was self-reported using a standardized questionnaire. We identified 66 differentially methylated CpG sites associated with smoking, located in 38 genes. In most of these CpG sites, we observed a trend among those quitting smoking to recover methylation levels typical of never smokers. A CpG site located in a novel smoking-associated gene (cg06394460 in <i>LNX2</i>) was hypomethylated in current smokers. Moreover, we validated two previously reported CpG sites (cg05886626 in <i>THBS1</i>, and cg24838345 in <i>MTSS1</i>) for their potential relation to atherosclerosis and cancer diseases, using several different approaches: CpG site methylation, gene expression, and plasma protein level determinations. Smoking was also associated with higher <i>THBS1</i> gene expression but with lower levels of thrombospondin-1 in plasma. Finally, we identified differential methylation regions in 13 genes and in four non-coding RNAs. In summary, this study replicated previous findings and identified and validated a new CpG site located in <i>LNX2</i> associated with smoking.</p

Adjusted means for the 2 aggregated dimensions of health-related quality of life by baseline categories of adherence to a traditional Mediterranean diet.

<p>The PREDIMED-Plus trial.</p

Mean differences in the health-related quality of life dimensions according to baseline adherence to the traditional Mediterranean diet in the PREDIMED-Plus trial.

<p>PR-Physical role BP-Bodily pain GH-General health PF-Physical functioning VT-Vitality SF- Social function RE-Emotional Role MH-Mental Health Aggr Physical Physical aggregated Aggr Mental- Mental Aggregated.</p