Effect of detritus decomposition time on Aedes aegypti oviposition site selection and immature developmental success

The abundance of the mosquito Aedes aegypti in larval habitats (containers with water) depends on the selection of oviposition sites by the females and on the subsequent survival of the immatures. Both can be affected by factors such as the decomposition time of the detritus, which determines the number of microorganisms (larval food) in the habitat. Females would be expected to select sites where larvae have the best performance to maximize their fitness. The aim of this work is to study the relationship between the selection of oviposition sites in response to detritus age, and the survival of immatures in these sites. To achieve this, an oviposition study and an immature development study were performed. In both studies, three treatments with different decomposition times of detritus in water (3, 15, and 45 days) were used.Para acceder a la videoconferencia completa, hacer clic en "Enlace externo".Sociedad Latinoamericana de Ecología de Vectore

Photoperiod affects female life history traits in temperate populations of Aedes aegypti from South America

In many insects, short photoperiods induce females to lay diapausing eggs, which are associated with a reduction in female fecundity, and/or with an increase in their growth rate, allowing for a larger size at adulthood. The effects of short photoperiods may also differ among populations, depending on the different selection pressures imposed by the winter conditions. Thus, this study aimed to experimentally assess the effects of short photoperiods on Aedes aegypti, an invasive mosquito species that also vectors several viral diseases. We compared life history parameters of the females reared from the first larval instar under contrasting photoperiods, in two populations from the temperate region of Argentina, one with a milder winter (BA) and the other with a harsher winter (SB). The results showed a significant increase in the time to oviposition, a trend to a larger size of females (significant only for SB) and higher inhibition of egg hatching (more pronounced in SB), in response to short photoperiods. No differences in fecundity were detected among treatments. The different reaction norms of the populations in female body size and egg hatching response suggest an adaptation to local conditions, which might favour a further expansion of A. aegypti towards colder climates.Fil: Campos, Raul Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Di Battista, Cristian Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: de Majo, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Fischer, Sylvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentin

Potential of Core-Collapse Supernova Neutrino Detection at JUNO

JUNO is an underground neutrino observatory under construction in Jiangmen, China. It uses 20kton liquid scintillator as target, which enables it to detect supernova burst neutrinos of a large statistics for the next galactic core-collapse supernova (CCSN) and also pre-supernova neutrinos from the nearby CCSN progenitors. All flavors of supernova burst neutrinos can be detected by JUNO via several interaction channels, including inverse beta decay, elastic scattering on electron and proton, interactions on C12 nuclei, etc. This retains the possibility for JUNO to reconstruct the energy spectra of supernova burst neutrinos of all flavors. The real time monitoring systems based on FPGA and DAQ are under development in JUNO, which allow prompt alert and trigger-less data acquisition of CCSN events. The alert performances of both monitoring systems have been thoroughly studied using simulations. Moreover, once a CCSN is tagged, the system can give fast characterizations, such as directionality and light curve

Detection of the Diffuse Supernova Neutrino Background with JUNO

As an underground multi-purpose neutrino detector with 20 kton liquid scintillator, Jiangmen Underground Neutrino Observatory (JUNO) is competitive with and complementary to the water-Cherenkov detectors on the search for the diffuse supernova neutrino background (DSNB). Typical supernova models predict 2-4 events per year within the optimal observation window in the JUNO detector. The dominant background is from the neutral-current (NC) interaction of atmospheric neutrinos with 12C nuclei, which surpasses the DSNB by more than one order of magnitude. We evaluated the systematic uncertainty of NC background from the spread of a variety of data-driven models and further developed a method to determine NC background within 15\% with {\it{in}} {\it{situ}} measurements after ten years of running. Besides, the NC-like backgrounds can be effectively suppressed by the intrinsic pulse-shape discrimination (PSD) capabilities of liquid scintillators. In this talk, I will present in detail the improvements on NC background uncertainty evaluation, PSD discriminator development, and finally, the potential of DSNB sensitivity in JUNO

Effects of the short- and long-term accumulation of detritus in larval habitats on life history traits of Aedes aegypti in temperate Argentina

Aedes aegypti larvae that develop in containers largely depend on plant detritus as a source of nutritional resources. However, few studies have evaluated the performance of immature individuals under natural amounts and quality of this food source. Here, we aimed to assess the effect of the variation in the accumulation time and amount of detritus on life history traits of Ae. aegypti under semi-field conditions. Ae. aegypti larvae were raised with detritus collected in different sites to represent natural variability in its amount, simulating short (28 days) and long (70 days) accumulation. A control with optimal food conditions (yeast) was included. Survival, development time and wing length of adults were compared among treatments. Survival was relatively high in all treatments. Development time was similar among treatments but significantly longer and more variable in containers with the lowest detritus amounts. Wing lengths were smaller in the treatments with detritus than in the control, especially in females. The results support the hypothesis that, in a temperate region, Ae. aegypti larvae may have a nutritional limitation, at least in some containers, and emphasize the importance of performing experiments that simulate the environmental conditions to which individuals are exposed in nature.Fil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Fischer, Sylvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentin

Egg hatching and survival of immature stages of Aedes aegypti (Diptera: Culicidae) under natural temperature conditions during the cold season in Buenos Aires, Argentina

In temperate regions, the seasonal dynamics of Aedes aegypti (L) (Diptera: Culicidae) is mainly influenced by temperature. It is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. The aim of the current study was to assess egg hatching of Ae. aegypti during the winter in Buenos Aires city (Argentina), and analyze the survival of immature stages. The experiments consisted of immersing eggs and studying the development of immature stages of cohorts from June and September under natural temperature conditions. The proportion of hatched eggs was compared between weeks of immersion and related to environmental variables. Survival was compared among cohorts and the development rate was related to the mean temperature during development. The results showed that, with few exceptions, egg hatching was over 45% during the winter period. The proportion of hatched eggs was positively associated with immersion temperature, pre-immersion temperature and photoperiod. The immature stages completed the development during the cold season, with a trend toward increased survival of late-hatching cohorts. Survival was 30% at 13.2 °C and above 90% at 20°C, whereas the development time at low temperatures was 49.4 d at 13.2°C and 17.7 d at 20°C. The high hatching and survival compared with other studies suggest that the local population might be adapting to winter conditions. The anticipated emergence of adults would be adaptive if they are able to reproduce successfully in the early spring.Fil: de Majo, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Fischer, Sylvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentin

Delayed mortality effects of cold fronts during the winter season on Aedes aegypti in a temperate region

The expansion of the invasive mosquito Aedes aegypti L. (Diptera: Culicidae) towards temperate regions in the Americas is causing concern because of its public health implications. As for other insects, the distribution limits of Ae. aegypti have been suggested to be related to minimum temperatures and to be controlled mainly by cold tolerance. The aim of this study was to assess the daily mortality of immature stages of Ae. aegypti under natural winter conditions in Buenos Aires, Argentina, in relation to preceding thermal conditions. The experiment was performed outdoors, and one cohort of larvae was started each week for 16 weeks, and reared up to the emergence of the adults. Three times a week, larvae, pupae and emerged adults were counted, and these data were used to calculate the daily mortality of larvae, pupae and adults and to analyze their relationship with thermal conditions. The results showed that mortality was generally low, with a few peaks of high mortality after cold front events. The mortality of pupae and larvae showed a higher correlation with the cooling degree hours of previous days than with the minimum, maximum or mean temperatures. Pupae and adults showed to be more vulnerable to low temperatures than larvae. A delay in mortality was observed in relation to the low temperature events, with a proportion of individuals dying in a later stage after the end of the cold front. These results suggest that thermal conditions during cold fronts in Buenos Aires are close to the tolerance limit of the local Ae. aegypti population. The wide range of responses of different individuals suggests that low winter temperatures may constitute a selective force, leading the population to a higher tolerance to low temperatures, which might favor the further expansion of this species towards colder regions.Fil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: de Majo, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Fischer, Sylvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentin

Adaptation to temperate climates: Evidence of photoperiod-induced embryonic dormancy in Aedes aegypti in South America

Dormancy is a developmental arrest in arthropods, in response to unfavorable conditions in temporally varying environments. In Aedes aegypti, the supposed inability of eggs to inhibit hatching has been used to explain the restriction of this species to tropical and subtropical regions. However, the geographic range of Ae. aegypti is constantly expanding towards temperate regions. Thus, the aim of the present study was to assess the ability of Ae. aegypti individuals from a temperate region (Buenos Aires City, Argentina) to enter photoperiod induced dormancy. To this end, we exposed both the parental generation and the eggs to short-day (SD: 10L:14D) and long-day (LD: 14L:10D) photoperiods, and studied the temporal variation in egg hatching. The experiment consisted of 28 treatment combinations of three factors: parental photoperiod (SD or LD), egg storage photoperiod (SD or LD), and age of eggs (14, 28, 42, 56, 70, 91, and 112 days). The results showed a lower hatching response with the SD parental photoperiod, and a trend to higher hatching with longer egg storage time in all photoperiod treatment combinations. The egg storage photoperiod showed no effect on egg hatching. In both parental photoperiod treatments, egg replicates of most ages from different females showed a large variability, with some replicates with lowest hatching response and others with highest hatching response. Our results show the ability of Ae. aegypti to inhibit egg hatching in response to a short-day photoperiod, which could allow the further expansion of this species to regions with colder winters.Fil: Fischer, Sylvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: de Majo, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Di Battista, Cristian Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Loetti, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Campos, Raul Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentin

Photoperiod induced effects on female fecundity and egg diapause in Aedes aegypti from a temperate region of South America

Mosquito populations in temperate regions are confronted with unfavorable conditions such as low winter temperatures. These conditions are anticipated by a short photoperiod, which is a major cue to induce changes in the life history of insects to survive these conditions. So far, no effects of photoperiod on the female fecundity or on eggs diapause of Aedes aegypti are known, despite the fact that this species is expanding to temperate regions. Thus, the aim of the present study was to assess the effects of photoperiod on the fecundity of females and on their ability to lay diapausing eggs in a population of Ae. aegypti from a temperate region (Buenos Aires City, Argentina). To this end, we exposed both the parental generation and the eggs to short-day (SD: 10L:14D) and long-day (LD: 14L:10D) photoperiods, and studied the fecundity of females, the hatching and mortality of eggs. Egg hatching and mortality were assessed for different eggs ages (from 14 to 112 days). The individuals from the SD photoperiod showed a lower fecundity and laid eggs with lower hatching response. A trend to higher hatching with increasing egg age was recorded in all treatments. Eggs mortality was also lower in the SD photoperiod. Our results show changes in female fecundity in response to photoperiod. Furthermore, the inhibition of hatching and the lower mortality of SD eggs suggest that they are in diapause, an ability that has not been reported previously in Ae. aegypti, which would explain its expansion to temperate regions.Fil: Di Battista, Cristian Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: de Majo, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Montini, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Loetti, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Fischer, Sylvia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Campos, Raul Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaGnatwork Brasil '19Belo HorizonteBrasilGnatworkUniversidade Federal De Minas Gerai