Extremal polynomials in stratified groups

We introduce a family of extremal polynomials associated with the prolongation of a stratified nilpotent Lie algebra. These polynomials tre related to a new algebraic characterization of abnormal sub-Riemannian extremals in stratified nilpotent Lie groups. They satisfy a set of remarkable structure relations that are used to integrate the adjoint equations, in both normal and abnormal case

Breeze analysis by mast and sodar measurements

During the year 1993, field measurements were carried out in a meteorological station located in the neighbourhood of Rome, 10 km from the coast (Tyrrhenian Sea). The monitoring station is composed of a 30 m mast and a three-axial Doppler sodar. A statistical analysis of data has been made in order to obtain the main parameters utilised by the dispersion model. Hourly, seasonal and conditional averages showed the strong influence of sea and land breeze circulation on the local characteristics of the atmospheric boundary layer. Such an aspect has to be considered in the numerical predictions of pollutant dispersion

A new subfamily of fungal subtilases: structural and functional analysis of a Pleurotus ostreatus member

Pleurotus ostreatus produces several extracellular proteases which are believed to be involved in the regulation of the ligninolytic activities of this fungus. Recently, purification and characterization of the most abundant P. ostreatus extracellular protease (PoSl) have been reported. The sequence of the posl gene and of the corresponding cDNA has been determined, allowing the identification of its pre- and pro-sequences. A mature protein sequence has been verified by mass spectrometry mapping, the N-glycosylation sites have been identified and the glycosidic moieties characterized. Mature PoSl shows a cleaved peptide bond in the C-terminal region, which remains associated with the catalytic domain in a non-covalent complex. Reported results indicate that this enzyme is involved in the activation of other P. ostreatus secreted proteases, thus suggesting its leading role in cascade activation mechanisms. Analyses of the PoSl sequence by homology search resulted in the identification of a DNA sequence encoding a new protease, homologous to PoSl, in the Phanerochaete chrysosporium genome. A new subgroup of subtilisin-like proteases, belonging to the pyrolysin family, has been defined, which includes proteases from ascomycete and basidiomycete fungi

The regions of the sequence most exposed to the solvent within the amyloidogenic state of a protein initiate the aggregation process.

Formation of misfolded aggregates is an essential part of what proteins can do. The process of protein aggregation is central to many human diseases and any aggregating event needs to be prevented within a cell and in protein design. In order to aggregate, a protein needs to unfold its native state, at least partially. The conformational state that is prone to aggregate is difficult to study, due to its aggregating potential and heterogeneous nature. Here, we use a systematic approach of limited proteolysis, in combination with electrospray ionisation mass spectrometry, to investigate the regions that are most flexible and solvent-exposed within the native, ligand-bound and amyloidogenic states of muscle acylphosphatase (AcP), a protein previously shown to form amyloid fibrils in the presence of trifluoroethanol. Seven proteases with different degrees of specificity have been used for this purpose. Following exposure to the aggregating conditions, a number of sites along the sequence of AcP become susceptible to proteolytic digestion. The pattern of proteolytic cleavages obtained under these conditions is considerably different from that of the native and ligand-bound conformations and includes a portion within the N-terminal tail of the protein (residues 6-7), the region of the sequence 18-23 and the position 94 near the C terminus. There is a significant overlap between the regions of the sequence found to be solvent-exposed from the present study and those previously identified to be critical in the rate-determining steps of aggregation from protein engineering approaches. This indicates that a considerable degree of solvent exposure is a feature of the portions of a protein that initiate the process of aggregation

Ochrobactrum sp. MPV1 from a dump of roasted pyrites can be exploited as bacterial catalyst for the biogenesis of selenium and tellurium nanoparticles

Background: Bacteria have developed different mechanisms for the transformation of metalloid oxyanions to non-toxic chemical forms. A number of bacterial isolates so far obtained in axenic culture has shown the ability to bioreduce selenite and tellurite to the elemental state in different conditions along with the formation of nanoparticles-both inside and outside the cells-characterized by a variety of morphological features. This reductive process can be considered of major importance for two reasons: firstly, toxic and soluble (i.e. bioavailable) compounds such as selenite and tellurite are converted to a less toxic chemical forms (i.e. zero valent state); secondly, chalcogen nanoparticles have attracted great interest due to their photoelectric and semiconducting properties. In addition, their exploitation as antimicrobial agents is currently becoming an area of intensive research in medical sciences. Results: In the present study, the bacterial strain Ochrobactrum sp. MPV1, isolated from a dump of roasted arsenopyrites as residues of a formerly sulfuric acid production near Scarlino (Tuscany, Italy) was analyzed for its capability of efficaciously bioreducing the chalcogen oxyanions selenite (SeO32-) and tellurite (TeO32-) to their respective elemental forms (Se0 and Te0) in aerobic conditions, with generation of Se- and Te-nanoparticles (Se- and TeNPs). The isolate could bioconvert 2 mM SeO32- and 0.5 mM TeO32- to the corresponding Se0 and Te0 in 48 and 120 h, respectively. The intracellular accumulation of nanomaterials was demonstrated through electron microscopy. Moreover, several analyses were performed to shed light on the mechanisms involved in SeO32- and TeO32- bioreduction to their elemental states. Results obtained suggested that these oxyanions are bioconverted through two different mechanisms in Ochrobactrum sp. MPV1. Glutathione (GSH) seemed to play a key role in SeO32- bioreduction, while TeO32- bioconversion could be ascribed to the catalytic activity of intracellular NADH-dependent oxidoreductases. The organic coating surrounding biogenic Se- and TeNPs was also characterized through Fourier-transform infrared spectroscopy. This analysis revealed interesting differences among the NPs produced by Ochrobactrum sp. MPV1 and suggested a possible different role of phospholipids and proteins in both biosynthesis and stabilization of such chalcogen-NPs. Conclusions: In conclusion, Ochrobactrum sp. MPV1 has demonstrated to be an ideal candidate for the bioconversion of toxic oxyanions such as selenite and tellurite to their respective elemental forms, producing intracellular Se- and TeNPs possibly exploitable in biomedical and industrial applications.[Figure not available: see fulltext.

Exploring the mechanism of formation of native-like and precursor amyloid oligomers for the native acylphosphatase from Sulfolobus solfataricus

Over 40 human diseases are associated with the formation of well-defined proteinaceous fibrillar aggregates. Since the oligomers precursors to the fibrils are increasingly recognized to be the causative agents of such diseases, it is important to elucidate the mechanism of formation of these early species. The acylphosphatase from Sulfolobus solfataricus is an ideal system as it was found to form, under conditions in which it is initially native, two types of prefibrillar aggregates: (1) initial enzymatically active aggregates and (2) oligomers with characteristics reminiscent of amyloid protofibrils, with the latter originating from the structural reorganization of the initial assemblies. By studying a number of protein variants with a variety of biophysical techniques, we have identified the regions of the sequence and the driving forces that promote the first aggregation phase and show that the second phase consists in a cooperative conversion involving the entire globular fol

Intramyocardial hemorrhage: An enigma for cardiac MRI?

Cardiovascular magnetic resonance (CMR) is a useful noninvasive technique for determining the presence of microvascular obstruction (MVO) and intramyocardial hemorrhage (IMH), frequently occurring in patients after reperfused myocardial infarction (MI). MVO, or the so-called no-reflow phenomenon, is associated with adverse ventricular remodeling and a poor prognosis during follow-up. Similarly, IMH is considered a severe damage after revascularization by percutaneous primary coronary intervention (PPCI) or fibrinolysis, which represents a worse prognosis. However, the pathophysiology of IMH is not fully understood and imaging modalities might help to better understand that phenomenon. While, during the past decade, several studies examined the distribution patterns of late gadolinium enhancement with different CMR sequences, the standardized CMR protocol for assessment of IMH is not yet well established. The aim of this review is to evaluate the available literature on this issue, with particular regard to CMR sequences. New techniques, such as positron emission tomography/magnetic resonance imaging (PET/MRI), could be useful tools to explore molecular mechanisms of the myocardial infarction healing process

Theoretical model and computacional procedure to evaluate the NSM FRP strips shear strength contribution to a RC beam

This paper presents a closed-form procedure to evaluate the shear strength contribution provided to a Reinforced Concrete (RC) beam by a system of Near Surface Mounted (NSM) Fiber Reinforced Polymer (FRP) strips. This procedure is based on the evaluation of: a) the constitutive law of the average-available-bond-length NSM FRP strip effectively crossing the shear crack and b) the maximum effective capacity it can attain during the loading process of the strengthened beam. Due to complex phenomena, such as: a) interaction between forces transferred through bond to the surrounding concrete and the concrete fracture, and b) interaction among adjacent strips, the NSM FRP strip constitutive law is largely different than the linear elastic one characterizing the FRP behavior in tension. Once the constitutive law of the average-available-bond-length NSM strip is reliably known, its maximum effective capacity can be determined by imposing a coherent kinematic mechanism. The self-contained and ready-to-implement set of analytical equations and logical operations is presented along with the main underlying physical-mechanical principles and assumptions. The formulation proposed is appraised against some of the most recent experimental results, and its predictions are also compared with those obtained by a recently developed more sophisticated model.Fundação para a Ciência e a Tecnologia (FCT) - CUTINEMO - Carbon fiber laminates applied according to the near surface mounted technique to increase the flexural resistance to negative moments of continuous reinforced concrete structures” (PTDC/ECM/73099/2006

NSM FRP strips shear strength contribution to a RC beam : a design procedure

This paper presents a closed-form procedure to evaluate the shear strength contribution provided to a Reinforced Concrete (RC) beam by a system of Near Surface Mounted (NSM) Fiber Reinforced Polymer (FRP) strips. This procedure is based on the evaluation of: a) the constitutive law of the average-available-bond-length NSM FRP strip effectively crossing the shear crack and b) the maximum effective capacity it can attain during the loading process of the strengthened beam. Due to complex phenomena, such as: a) interaction between forces transferred through bond to the surrounding concrete and concrete fracture, and b) interaction among adjacent strips, the NSM FRP strip constitutive law is largely different than the linear elastic one characterizing the FRP behavior in tension. Once the constitutive law of the average-available-bond-length NSM strip is reliably known, its maximum effective capacity can be determined by imposing a coherent kinematic mechanism. The self-contained and ready-to-implement set of analytical equations and logical operations is presented along with the main underlying physical-mechanical principles and assumptions. The formulation proposed is appraised against some of the most recent experimental results and its predictions are also compared with those obtained by a recently developed more sophisticated model.(undefined

Influence of the concrete mechanical properties on the efficacy of the shear strengthening intervention on RC beams by NSM technique

The occurrence of a failure mode, different from debonding, consisting on the detachment, from the beam core, of “two concrete lateral walls” containing the glued laminates, highlights the paramount importance of concrete mechanical properties on the effectiveness of NSM shear strengthening technique. A new mechanical-analytical interpretation of the phenomenon affecting the ultimate behaviour of RC beams NSM-strengthened in shear is presented along with the main findings. This approach takes into account the possibility that the shear strengthening contribution of the NSM laminates can be limited by the three following failure modes: debonding, concrete tensile fracture and laminates’ tensile rupture. The interaction between laminates can be also accounted for. The proposed mechanical interpretation of the NSM laminates behaviour can be extended to NSM rods.Fundação para a Ciência e a Tecnologia (FCT) - programa “CUTINSHEAR - Performance assessment of an innovative structural FRP strengthening technique using an integrated system based on optical fiber sensors” s, POCTI/ECM/59033/2004. This work has been partially carried out under the program “Dipartimento di Protezione Civile – Consorzio RELUIS”, signed on 2005-07-11 (n. 540), Research Line 8, whose financial support is greatly appreciatePrograma “Dipartimento di Protezione Civile – Consorzio RELUIS” nº 540Research Line 8Empreiteiros CasaisS&P®Secil (Unibetão, Braga)Degussa