Reemergence of Oropouche Fever, Northern Brazil

Oropouche fever has reemerged in Parauapebas and Porto de Moz municipalities, Pará State, Brazil. Serologic analysis (immunoglobulin M–ELISA) and virus isolation confirmed Oropouche virus (OROV) in both municipalities. Nucleotide sequencing of 2 OROV isolates from each location indicated genotypes I (Parauapebas) and II (Porto de Moz) in Brazil

New records of culicidae species with medical importance in the state of Paraíba, Brazil

State University of Paraíba. Department of Biology. Center for Integrated Pest Management. Laboratory of Entomology. Campina Grande, PB, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Entomologia. Ananindeua, PA, Brasil.State University of Paraíba. Department of Biology. Center for Integrated Pest Management. Laboratory of Entomology. Campina Grande, PB, Brazil.This paper aimed to investigate the Culicidae fauna in the state of Paraíba and review the possible pathogens vector capacity of captured specimens. The catches occurred on November from day 17 to 21, 2014 in Mata do Buraquinho, João Pessoa/PB and were performed with the use of aerial insect net and an oral aspirator. There were 88 Culicidae specimens collected from the Buraquinho Forest. This is the first report of Aedes fulvithorax, A. scapularis, A. serratus, A. taeniorhynchus, Anopheles nimbus, Coquillettidia albicosta, C. venezuelensis, Limatus durhamii and Mansonia titillans, also genus Wyeomyia in Paraíba state. The capacity of these mosquitoes to transmit diseases, mainly arboviruses, outlines the need for epidemiological surveillance in the state

Fauna de culicidae nas áreas rurais de Porto Velho e o primeiro recorde de Mansonia (Mansonia) flaveola (Coquillet, 1906), para o Estado de Rondônia, Brasil

Ministry of Health; Pan American Health Organization-OPAS; CNPq; CAPESUniversidade Federal do Rio Grande do Norte. Centro de Biociências. Laboratório de Insetos e Vetores. Natal, RN, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Parasitologia Médica. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Ecologia Química de Insetos Vetores. Belo Horizonte, MG, Brasil.Introdução: O conhecimento da fauna de culicídeos em estados da Amazônia Brasileira auxilia no conhecimento da bionomia dos insetos obtidos, além de possibilitar a observação de modificações da fauna ao decorrer do tempo. Métodos: As capturas dos culicídeos foram realizadas em área extradomiciliar de duas zonas rurais de Porto Velho, durante junho e julho, nos anos de 2007 e 2008, com a armadilha BG-Sentinel®. Resultados: Foram coletados 10.695 culicídeos, distribuídos em nove gêneros: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia e Wyeomyia. Conclusões: Foi registrada pela primeira vez no estado a presença de Mansonia (Mansonia) flaveola.Introduction: Knowledge concerning the fauna of Culicidae in the Brazilian Amazon States contributes to current understanding of the bionomics of the insects collected and makes it possible to observe changes in the fauna over time. METHODS: The Culicidae were captured with a BG-Sentinel® trap in extra-domiciliary area of two rural regions of Porto Velho in June and July of 2007 and 2008. RESULTS: A total of 10,695 Culicidae was collected, belonging to nine genera: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia and Wyeomyia. CONCLUSIONS: The presence of Mansonia (Mansonia) flaveola was recorded in the State of Rondônia for the first time

Description of mitochon genome and phylogenetic considerations of Sabethes bipartipes, Sabethes cyaneus, Sabethes quasicyaneus, and Sabethes tarsopus (Diptera: Culicidae)

Federal University of Pará. Institute of Biological Sciences. Center of Genomics and System Biology. Post-graduate program in Genetics and Molecular Biology, Laboratory of Genomic and Bioinformatics. Belém, PA, BrazilState of Pará University. Post-graduate program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences. Belém, PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilState of Pará University. Post-graduate program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences. Belém, PA, BrazilState of Pará University. Post-graduate program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilState of Pará University. Post-graduate program in Parasitary Biology in the Amazon, Center of Biological and Health Sciences. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilThe genus Sabethes (Diptera: Culicidae) comprises species of great epidemiological relevance, particularly involved in transmission cycles of the Yellow fever virus in South America. Given the unavailability of information related to aspects of evolutionary biology and molecular taxonomy of species of this genus of mosquitoes, we report here the first sequencing of the mitochondrial genomes of Sabethes bipartipes, Sabethes cyaneus, Sabethes tarsopus, and Sabethes quasicyaneus. The sequences obtained showed an average length of 14,920 bp, comprising 37 functional genes (13 PCGs, 22 tRNA, and 02 rRNA). The phylogenies reconstructed by Maximum likelihood and Bayesian inference methods, based on the concatenated sequences of all 13 PCGs, produced similar topologies and strongly supported the monophyletic relationship between the Sabethes subgenera, corroborating the known taxonomic classification based on aspects of the external morphology of the taxa assessed. The data and information produced from the Sabethes species evaluated here may be useful for future taxonomic and evolutionary studies of the genus, as well as the Culicidae family

Ecological aspects of potential arbovirus vectors (Diptera: Culicidae) in an urban landscape of Southern Amazon, Brazil

Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brazil.Secretaria de Estado de Saúde de Mato Grosso. Escritório Regional de Saúde de Sinop. Sinop, MT, Brazil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brazil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brazil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências Farmacêuticas. Araraquara, São Paulo, Brazil.Universidade do Estado de Mato Grosso. Programa de Pós-Graduação em Ciências Ambientais. Cáceres, MT, Brazil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brazil.Amazon has been under intense human pressure, especially in the so-called “Arc of Deforestation” in the Eastern and Southern regions. Changes in biodiversity due to landscape disturbance in municipalities at the Arc of Deforestation are likely to impact mosquito species leading to the potential for emergence of arboviruses. Our aim was to describe the composition and structure of culicids in Sinop, a municipality located in the Southern Amazon at the State of Mato Grosso, as well as their presence in urban habitats, analyzing climate variables and potential risk of arbovirus transmission. Mosquitoes were collected in four habitats located in urban environments for 12 months. We recovered 5,210 adult mosquitoes from mosquito traps. Among the 33 species identified, 19 are associated with arbovirus transmission. Higher abundance, richness, diversity, and equitability of mosquito species were observed in urban forest parks and in new neighborhoods located in the outskirts of the city, nearby forest fragments and agricultural areas. As the environment consolidates as urban, both abundance and richness decrease. Highly urbanized neighborhoods had higher dominance of Culex quinquefasciatus, a mosquito associated with different arboviruses, including West Nile and Saint Louis encephalitis virus. The medically important species Cx. declarator, Cx. (Melanoconion) ssp., Aedeomyia squamipennis and Aedes scapularis were found in the four habitats. It is possible that these mosquitoes are adapting to different habitats and as a consequence, they are reaching urbanized areas. Mosquito abundance was higher in the days of high temperature and low precipitation in urban forest parks and in highly urbanized neighborhoods. These results suggest that Sinop region is a key area for surveillance of arbovirus vectors