A mechanistic study on the phototoxicity of atorvastatin: singlet oxygen generation by a phenanthrene-like photoproduct

Atorvastatin calcium (ATV) is one of the most frequently prescribed drugs worldwide. Among the adverse effects observed for this lipid-lowering agent, clinical cases of cutaneous adverse reactions have been reported and associated with photosensitivity disorders. Previous work dealing with ATV photochemistry has shown that exposure to natural sunlight in aqueous solution leads to photoproducts resulting from oxidation of the pyrrole ring and from cyclization to a phenanthrene derivative. Laser flash photolysis of ATV, at both 266 and 308 nm, led to a transient spectrum with two maxima at λ ) 360 and λ ) 580 nm (τ ) 41 μs), which was assigned to the primary intermediate of the stilbene-like photocyclization. On the basis of the absence of a triplet-triplet absorption, the role of the parent drug as singlet oxygen photosensitizer can be discarded. By contrast, a stable phenanthrene-like photoproduct would be a good candidate to play this role. Laser flash photolysis of this compound showed a triplet-triplet transient absorption at λmax ) 460 nm with a lifetime of 26 μs, which was efficiently quenched by oxygen (kq ) 3 ((0.2) × 109 M-1 s-1). Its potential to photosensitize formation of singlet oxygen was confirmed by spin trapping experiments, through conversion of TEMP to the stable free radical TEMPO. The photoreactivity of the phenanthrene-like photoproduct was investigated using Trp as a marker. The disappearance of the amino acid fluorescence (λmax ) 340 nm) after increasing irradiation times at 355 nm was taken as a measurement of photodynamic oxidation. To confirm the involvement of a type II mechanism, the same experiment was also performed in D2O; this resulted in a significant enhancement of the reaction rate. On the basis of the obtained photophysical and photochemical results, the phototoxicity of atorvastatin can be attributed to singlet oxygen formation with the phenanthrene-like photoproduct as a photosensitizer

Morphological Characterization of Some Local Varieties of Fig (Ficus carica L.) Cultivated in Southern Italy

Figs (Ficus carica L.) are ancient fruits of the Mediterranean basin. In Southern Italy, they are particularly important in the traditional course of local cuisine. In Southern Italy, fig trees are rarely cultivated in specialized orchards but are present in association with other fruit trees (for example, olive, almond, pear, pomegranate, and grapevine). These mixed orchards are particularly important in the traditional agroecosystems of the south of Italy. This study reports preliminary results on the local fig variety’s leaf morphological characterization, aiming to elucidate the presence of synonymousness or homonymy for in situ and ex situ conservation and further exploitation. A field survey was carried out during the summer of 2018 in some areas of the Basilicata district. Thirty local putative varieties were collected, and each of them was identified by GPS coordinates and recorded photographically. Moreover, they were cataloged with the name of the Municipality of origin, year, details of growing location (main crop, mixed orchard, gardens, and single plants), approximate age, and the local name supplied by the donor. All relevant information was included in the accession code. Leaf samples were collected from each accession from medium-length shoots. A digital image of each leaf sample was captured using a digital camera. Leaf morphometric traits were recorded using ImageJ and statistically analyzed using the software PAST 4.11 to discriminate among fig accessions. The multivariate morphometric approach applied correctly classified more than 90% of the leaves and helped to discriminate among accession. Moreover, linear discriminant analysis helped to recognize the presence of different synonymousness and homonymy of different accessions. The results revealed that measured leaf morphometric aided by image analysis could be a simple and inexpensive accessions classification tool

Safety profile of paediatric COVID-19 vaccines: An analysis of the US Vaccine Adverse Event Reporting System

Aim: To provide further evidence on the safety profile of COVID-19 vaccines in paediatrics by analysing the spontaneous reports of adverse effects related to these vaccines.Methods: Reports related to US paediatric population (from 0 to 17 years) vaccinated with authorised COVID-19 vaccines were extracted from Vaccine Adverse Event Reporting System from December 2020 to 17 November 2022. We conducted a descriptive analysis of Adverse Events Following Immunization (AEFI), calculating reporting rate of serious AEFIs and focusing on myocarditis and Guillain-Barre Syndrome after mRNA COVID-19 vaccines.Results: Overall, 52 720 reports were retrieved: 77% (40541)-Pfizer-BioNTech, 19% (10083)-Moderna, a small proportion for other vaccines 4% (2096). Most of AEFIs were non-serious and listed in corresponding SPCs. Of serious AEFIs, 96% were related to the Pfizer-BioNTech vaccine. Roughly 91% (47874) were related to people from 6 to 17 years, a small percentage of 9% (4773) to the younger group (0-5 years). In both groups, most of the reports were related to mRNA vaccines and the percentage of AEFIs experienced by females were similar to males.Conclusions: Data showed that events most frequently reported were non-serious and listed in the corresponding SPCs, extending the evidence of safety of COVID-19 vaccines authorised in the United States in children

Phenotyping Key Fruit Quality Traits in Olive Using RGB Images and Back Propagation Neural Networks

To predict oil and phenol concentrations in olive fruit, the combination of back propagation neural networks (BPNNs) and contact-less plant phenotyping techniques was employed to retrieve RGB image-based digital proxies of oil and phenol concentrations. Fruits of cultivars (×3) differing in ripening time were sampled (∼10-day interval, ×2 years), pictured and analyzed for phenol and oil concentrations. Prior to this, fruit samples were pictured and images were segmented to extract the red (R), green (G), and blue (B) mean pixel values that were rearranged in 35 RGB-based colorimetric indexes. Three BPNNs were designed using as input variables (a) the original 35 RGB indexes, (b) the scores of principal components after a principal component analysis (PCA) pre-processing of those indexes, and (c) a reduced number (28) of the RGB indexes achieved after a sparse PCA. The results show that the predictions reached the highest mean R2 values ranging from 0.87 to 0.95 (oil) and from 0.81 to 0.90 (phenols) across the BPNNs. In addition to the R2, other performance metrics were calculated (root mean squared error and mean absolute error) and combined into a general performance indicator (GPI). The resulting rank of the GPI suggests that a BPNN with a specific topology might be designed for cultivars grouped according to their ripening period. The present study documented that an RGB-based image phenotyping can effectively predict key quality traits in olive fruit supporting the developing olive sector within a digital agriculture domain

Two-sided estimates of minimum-error distinguishability of mixed quantum states via generalized Holevo-Curlander bounds

We prove a concise factor-of-2 estimate for the failure rate of optimally distinguishing an arbitrary ensemble of mixed quantum states, generalizing work of Holevo [Theor. Probab. Appl. 23, 411 (1978)] and Curlander [Ph.D. Thesis, MIT, 1979]. A modification to the minimal principle of Cocha and Poor [Proceedings of the 6th International Conference on Quantum Communication, Measurement, and Computing (Rinton, Princeton, NJ, 2003)] is used to derive a suboptimal measurement which has an error rate within a factor of 2 of the optimal by construction. This measurement is quadratically weighted and has appeared as the first iterate of a sequence of measurements proposed by Jezek et al. [Phys. Rev. A 65, 060301 (2002)]. Unlike the so-called pretty good measurement, it coincides with Holevo's asymptotically optimal measurement in the case of nonequiprobable pure states. A quadratically weighted version of the measurement bound by Barnum and Knill [J. Math. Phys. 43, 2097 (2002)] is proven. Bounds on the distinguishability of syndromes in the sense of Schumacher and Westmoreland [Phys. Rev. A 56, 131 (1997)] appear as a corollary. An appendix relates our bounds to the trace-Jensen inequality.Comment: It was not realized at the time of publication that the lower bound of Theorem 10 has a simple generalization using matrix monotonicity (See [J. Math. Phys. 50, 062102]). Furthermore, this generalization is a trivial variation of a previously-obtained bound of Ogawa and Nagaoka [IEEE Trans. Inf. Theory 45, 2486-2489 (1999)], which had been overlooked by the autho

Hitting time for the continuous quantum walk

We define the hitting (or absorbing) time for the case of continuous quantum walks by measuring the walk at random times, according to a Poisson process with measurement rate $\lambda$. From this definition we derive an explicit formula for the hitting time, and explore its dependence on the measurement rate. As the measurement rate goes to either 0 or infinity the hitting time diverges; the first divergence reflects the weakness of the measurement, while the second limit results from the Quantum Zeno effect. Continuous-time quantum walks, like discrete-time quantum walks but unlike classical random walks, can have infinite hitting times. We present several conditions for existence of infinite hitting times, and discuss the connection between infinite hitting times and graph symmetry.Comment: 12 pages, 1figur

The effects of calcite silicon-mediated particle film application on leaf temperature and grape composition of Merlot (Vitis vinifera L.) vines under different irrigation conditions

This study examined whether the application of calcite-silicon mediated particle film (CaPF) at veraison can mitigate a drought-induced increase in leaf temperature on grapevine, thus contributing to improved leaf functionality, yield and grape composition traits. A total of 48 five-year-old Merlot (Vitis vinifera L.) vines grafted onto SO4 were grown (in 20 L PVC pots) under Mediterranean conditions (Southern Italy). The vines were pruned to two spurs with two winter buds irrigated daily to 100 % field capacity, and fertilised weekly. At veraison and using a 2×2 factorial experimental design, the two main factors, thermoregulation and water, were imposed at two levels: spraying with a thermoregulation compound (CaPF) and no spraying (NS); irrigation (WW) and drought stress (D)). A group of 24 vines was subjected to a 15-day drought period by receiving, every day, 25 % (D) of the daily water consumption of WW vines. The other 24 vines continued to be fully irrigated on a daily basis (WW). Twelve vines per group were sprayed (WW+CaPF, D+CaPF) with calcite-silicon mediate (3 % V/V) at the beginning of drought imposition, the remaining 24 vines were not sprayed (WW-NS, D-NS). Soil water moisture and stem water potential values were monitored from 11.30 to 13:30 nearly every week, and other vegetative and reproductive parameters were also measured. During the experiment, air temperature peaked at ≈35 °C at midday, VPD at about 3.7 kPa and PAR reached ≈2000 µmol m-2 s–1. Results show that in CaPF sprayed vines, leaf-air temperature differences were lower than in unsprayed vines in both irrigated and drought stressed groups. WW+CaPF vines retained significantly more leaf area and showed the highest value of accumulated vine transpiration. Calcite-silicon mediated particle film could enhance the resilience of grapevine to adverse environmental conditions and may contribute to preserve terroir elements in highly reputed wine grape growing areas. The study showed that foliar application of calcite silicon-mediated processed particles films can be used in arid regions to mitigate leaf temperatures in grapevines

Cetuximab induced cutaneous rash: Does it affect psychological well-being in colorectal cancer patients?

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