Inflammasomes NLRP3 and AIM2 in peri-implantitis: A cross-sectional study

Background: Inflammasome components NLRP3 and AIM2 contribute to inflammation development by the activation of caspase-1 and IL-1β. They have not been yet evaluated in samples from patients with active peri-implantitis. Thus, the aim of the present study is to analyze the expression of inflammasomes NLRP3 and AIM2 and subsequent caspase 1 and IL-1β assessing the microenvironment of leukocyte subsets in samples from patients with active peri-implantitis. Methods: Biopsies were collected from 33 implants in 21 patients being treated for peri-implantitis. Biopsies from gingival tissues from 15 patients with healthy periodontium were also collected for control. These tissues were evaluated through conventional histological stainings. Then, immunohistochemical detection was performed to analyze NLRP3, AIM2, caspase-1, and IL-1β and markers of different leukocyte subsets. PCR for inflammasomes and related genes was also done. Results: This manuscript reveals a high immunohistochemical and mRNA expression of NLRP3 and AIM2 inflammasomes, caspase-1, and IL-1β in biopsies collected from human peri-implantitis. The expression of the tested markers was significantly correlated with the increase in inflammatory infiltrate, probing depth, presence of biofilm, and bleeding on probing. In these peri-implantitis lesions, the area of biopsy tissue occupied by inflammatory infiltrate was intense while the area occupied by collagen was significantly lower. In comparison with periodontal healthy tissues, the inflammatory infiltrate was statistically significantly higher in the peri-implantitis biopsies and was mainly composed of plasma cells, followed by T and B lymphocytes. Conclusion: In human peri-implantitis, chronic inflammation can be explained in part by the action of IL-1β/ caspase 1 induced through NLRP3 and AIM2 inflammasome activation.Junta de Andalucía, Grant/Award Number: CTS-138CTS-1028; Universidad de Granada, Grant/Award Number: B-CTS- 504- UGR18Universidad de Granada/CBU

Diferentes modos de vida, diferentes dietas. Caries e isótopos estables en dos poblaciones burgalesas medievales

Se comparan dos poblaciones burgalesas medievales con cementerios excavados en roca: Palacios de la Sierra (siglos IX-XIII), núcleo con economía basada principalmente en ganadería y explotación maderera en un entorno frío y montañoso, y Santa María de Tejuela (siglos VIII-XI), con economía fundamentalmente agrícola en una planicie y clima más cálido. La hipótesis del estudio es que deberían existir diferencias en su dieta. Se han analizado los isótopos estables de C y N de 101 individuos y la patología máxilo-dentaria de 79. Palacios de la Sierra, muestra una dieta con un elevado consumo de proteínas animales, superior al de Santa María de Tejuela, y menor patología dental en general. Las diferencias son estadísticamente significativas entre ambos sexos en Palacios, donde destaca un elevado consumo de proteínas animales y una menor tasa de patología dental entre las mujeres. Los resultados obtenidos están en consonancia con la hipótesis del estudio. ABSTRACT: This study compares two medieval villages in the province of Burgos (Northern Spain). The first one, Palacios de la Sierra, is a settlement with an economy based mainly on animal husbandry and logging, dating between the 9th and the 13th centuries CE. It is located in a mountainous area characterized by a cold climate. The second site, Santa María de Tejuela, dated between the 8th and the early 11th centuries CE, is located in a flat area, with a temperate climate and its economy is based mainly on agriculture and livestock breeding. The working hypothesis of the study is that there must have been differences in the diet of the two populations given their different way of life and environment. Stable isotopes of carbon and nitrogen as well as maxillo-dental pathology have been analyzed in a total of 101 adult individuals, in which sex and age have been estimated. In total, the sample of Palacios de la Sierra consists of 16 males and 13 females and that of Santa María de Tejuela consists of 44 males and 28 females. Palacios de la Sierra shows a diet based on C3 plants with high consumption of animal proteins, whereas in the diet of Santa María de Tejuela C4 plants were introduced and the protein values are considerably lower. As for dental pathology, differences have been found between the two sites, with the population of Palacios de la Sierra exhibiting lower rates. Regarding the possible differences by sex, these are marked in Palacios, where females have a diet with a higher protein intake than males, and a lower rate of dental pathology. In Santa María de Tejuela, on the other hand, there are no significant differences by sex in the isotopic values, and males have better dental health. The results obtained in Palacios, especially among females, are consistent with those of a diet based on a significant consumption of animal proteins, possibly dairy products, and even similar to those of medieval elites of Castile. As for the results of Santa María de Tejuela, they are similar to those of other medieval rural settlements in the region and clearly different from those of Palacios de la Sierra

Diferentes modos de vida, diferentes dietas. Caries e isótopos estables en dos poblaciones burgalesas medievales

Se comparan dos poblaciones burgalesas medievales con cementerios excavados en roca: Palacios de la Sierra (siglos IX-XIII), núcleo con economía basada principalmente en ganadería y explotación maderera en un entorno frío y montañoso, y Santa María de Tejuela (siglos VIII-XI), con economía fundamentalmente agrícola en una planicie y clima más cálido. La hipótesis del estudio es que deberían existir diferencias en su dieta. Se han analizado los isótopos estables de C y N de 101 individuos y la patología máxilo-dentaria de 79. Palacios de la Sierra, muestra una dieta con un elevado consumo de proteínas animales, superior al de Santa María de Tejuela, y menor patología dental en general. Las diferencias son estadísticamente significativas entre ambos sexos en Palacios, donde destaca un elevado consumo de proteínas animales y una menor tasa de patología dental entre las mujeres. Los resultados obtenidos están en consonancia con la hipótesis del estudioEste trabajo se ha llevado a cabo dentro del Proyecto “Salud y alimentación en poblaciones rurales de la España Medieval” (HAR2016-75788-P) financiado por el Gobierno de España

Concordancia inter-examinador en la interpretación de hallazgo periodontales en radiografías panorámicas

Tesis (Odontólogo). -- Universidad de Cartagena. Facultad de Odontología. Departamento de Investigación, 2015Estimar el grado de concordancia inter-examinador en la interpretación de hallazgos periodontales en radiografías panorámicas en estudiantes de último año de la facultad de odontología de la Universidad de Cartagena. La concordancia entre los estudiantes observadores y el estándar de oro fue pobre. Lo que indica que los estudiantes no aplican los conocimientos obtenidos durante el pregrado de odontología, lo que puede llevar a un erróneo diagnóstico de enfermedad periodontal

Search for CPCP violation in D0^0→\to KS0^0_\mathrm{S}KS0^0_\mathrm{S} decays in proton-proton collisions at s\sqrt{s} = 13 TeV

International audienceA search is reported for charge-parity D$^0$$\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$$CP$ violation in D$^0$$\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$ decays, using data collected in proton-proton collisions at $\sqrt{s}$ = 13 TeV recorded by the CMS experiment in 2018. The analysis uses a dedicated data set that corresponds to an integrated luminosity of 41.6 fb$^{-1}$, which consists of about 10 billion events containing a pair of ẖadrons, nearly all of which decay to charm hadrons. The flavor of the neutral D meson is determined by the pion charge in the reconstructed decays D$^{*+}$$\to$ D$^0\pi^+$ and D$^{*-}$$\to$ D$^0\pi^-$. The D$^0$$\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$$CP$ asymmetry in D$^0$$\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$ is measured to be $A_{CP}$( K$^0_\mathrm{S}$K$^0_\mathrm{S}$) = (6.2 $\pm$ 3.0 $\pm$ 0.2 $\pm$ 0.8)%, where the three uncertainties represent the statistical uncertainty, the systematic uncertainty, and the uncertainty in the measurement of the D$^0$ $\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$ $CP$ asymmetry in the D$^0$ $\to$ K$^0_\mathrm{S}\pi^+\pi^-$ decay. This is the first D$^0$ $\to$ K$^0_\mathrm{S}$K$^0_\mathrm{S}$ $CP$ asymmetry measurement by CMS in the charm sector as well as the first to utilize a fully hadronic final state

Search for long-lived heavy neutrinos in the decays of B mesons produced in proton-proton collisions at s\sqrt{s} = 13 TeV

International audienceA search for long-lived heavy neutrinos (N) in the decays of \PB mesons produced in proton-proton collisions at $\sqrt{s}$ = 13 TeV is presented. The data sample corresponds to an integrated luminosity of 41.6 fb$^{-1}$ collected in 2018 by the CMS experiment at the CERN LHC, using a dedicated data stream that enhances the number of recorded events containing B mesons. The search probes heavy neutrinos with masses in the range 1 $\lt$$m_\mathrm{N}$$\lt$ 3 GeV and decay lengths in the range 10$^{-2}$$\lt$$c\tau$$\lt$ 10$^{4}$ mm, where $\tau_\mathrm{N}$ is the N proper mean lifetime. Signal events are defined by the signature B $\to$$\ell_\mathrm{B}$NX; N $\to$$\ell^{\pm} \pi^{\mp}$, where the leptons $\ell_\mathrm{B}$ and $\ell$ can be either a muon or an electron, provided that at least one of them is a muon. The hadronic recoil system, X, is treated inclusively and is not reconstructed. No significant excess of events over the standard model background is observed in any of the $\ell^{\pm}\pi^{\mp}$ invariant mass distributions. Limits at 95% confidence level on the sum of the squares of the mixing amplitudes between heavy and light neutrinos, $\vert V_\mathrm{N}\vert^2$, and on $c\tau$ are obtained in different mixing scenarios for both Majorana and Dirac-like N particles. The most stringent upper limit $\vert V_\mathrm{N}\vert^2$ $\lt$ 2.0$\times$10$^{-5}$ is obtained at $m_\mathrm{N}$ = 1.95 GeV for the Majorana case where N mixes exclusively with muon neutrinos. The limits on $\vert V_\mathrm{N}\vert^2$ for masses 1 $\lt$ $m_\mathrm{N}$ $\lt$ 1.7 GeV are the most stringent from a collider experiment to date

The CMS Statistical Analysis and Combination Tool: COMBINE

International audienceThis paper describes the COMBINE software package used for statistical analyses by the CMS Collaboration. The package, originally designed to perform searches for a Higgs boson and the combined analysis of those searches, has evolved to become the statistical analysis tool presently used in the majority of measurements and searches performed by the CMS Collaboration. It is not specific to the CMS experiment, and this paper is intended to serve as a reference for users outside of the CMS Collaboration, providing an outline of the most salient features and capabilities. Readers are provided with the possibility to run COMBINE and reproduce examples provided in this paper using a publicly available container image. Since the package is constantly evolving to meet the demands of ever-increasing data sets and analysis sophistication, this paper cannot cover all details of COMBINE. However, the online documentation referenced within this paper provides an up-to-date and complete user guide

Search for the Z boson decay to ττμμ\tau\tau\mu\mu in proton-proton collisions at s\sqrt{s} = 13 TeV

International audienceThe first search for the Z boson decay to $\tau\tau\mu\mu$ at the CERN LHC is presented, based on data collected by the CMS experiment at the LHC in proton-proton collisions at a center-of-mass energy of 13 TeV and corresponding to an integrated luminosity of 138 fb$^{-1}$. The data are compatible with the predicted background. For the first time, an upper limit at the 95% confidence level of 6.9 times the standard model expectation is placed on the ratio of the Z $\to$ $\tau\tau\mu\mu$ to Z $\to$ 4$\mu$ branching fractions. Limits are also placed on the six flavor-conserving four-lepton effective-field-theory operators involving two muons and two tau leptons, for the first time testing all such operators

Search for soft unclustered energy patterns in proton-proton collisions at 13 TeV

International audienceThe first search for soft unclustered energy patterns (SUEPs) is performed using an integrated luminosity of 138 fb$^{-1}$ of proton-proton collision data at $\sqrt{s}$ = 13 TeV collected in 2016-2018 by the CMS detector at the LHC. Such SUEPs are predicted by Hidden Valley models with a new, confining force with a large 't Hooft coupling. In events with boosted topologies, selected by high-threshold hadronic triggers, the multiplicity and sphericity of clustered tracks are used to reject the background from standard model quantum chromodynamics. With no observed excess of events over the standard model expectation, limits are set on the cross section for production via gluon fusion of a scalar mediator with SUEP-like decays

Search for the Z boson decay to ττμμ\tau\tau\mu\mu in proton-proton collisions at s\sqrt{s} = 13 TeV

International audienceThe first search for the Z boson decay to $\tau\tau\mu\mu$ at the CERN LHC is presented, based on data collected by the CMS experiment at the LHC in proton-proton collisions at a center-of-mass energy of 13 TeV and corresponding to an integrated luminosity of 138 fb$^{-1}$. The data are compatible with the predicted background. For the first time, an upper limit at the 95% confidence level of 6.9 times the standard model expectation is placed on the ratio of the Z $\to$ $\tau\tau\mu\mu$ to Z $\to$ 4$\mu$ branching fractions. Limits are also placed on the six flavor-conserving four-lepton effective-field-theory operators involving two muons and two tau leptons, for the first time testing all such operators