4 research outputs found
Water Flux Induced Reorientation of Liquid Crystals
It is well understood
that the adsorption of solutes at the interface
between a bulk liquid crystal phase and an aqueous phase can lead
to orientational or anchoring transitions. A different principle is
introduced here, whereby a transient reorientation of a thermotropic
liquid crystal is triggered by a spontaneous flux of water across
the interface. A critical water flux can be generated by the addition
of an electrolyte to the bulk aqueous phase, leading to a change in
the solvent activity; water is then transported through the liquid
crystal phase and across the interface. The magnitude of the spontaneous
water flux can be controlled by the concentration and type of solutes,
as well as the rate of salt addition. These results present new, previously
unappreciated fundamental principles that could potentially be used
for the design of materials involving transient gating mechanisms,
including biological sensors, drug delivery systems, separation media,
and molecular machines
Understanding Atomic-Scale Behavior of Liquid Crystals at Aqueous Interfaces
The ordered environment
presented by liquid crystals at interfaces
enables a range of novel functionalities that is only now beginning
to be exploited in applications ranging from light focusing devices
to biosensors. One key feature of liquid crystals is that molecular
events occurring at an interface propagate over large distances through
the bulk. In spite of their importance, our fundamental understanding
of liquid crystal–water and liquid crystal–air interfaces remains limited.
In this work, we present results from large-scale atomistic molecular
dynamics simulations on the organization of the nematic and isotropic
phases of the nitrile-containing mesogenic molecule 4-cyano-4′-pentylbiphenyl
(5CB) in the vicinity of vacuum and aqueous interfaces. Hybrid boundary
conditions are imposed by confining 5CB films between vacuum and an
aqueous medium to examine how those two types of interfaces influence
the specific structural arrangement and ordering of 5CB. Consistent
with experiments, our results indicate that 5CB exhibits homeotropic
anchoring at the vacuum interface, and planar alignment at aqueous
interfaces. Two-dimensional molecular dynamics potential of mean force
calculations and average polarization densities show that the polar
nitrile group of 5CB remains hydrated near the aqueous interface,
where it modulates the orientation of water molecules. Estimates of
the anchoring strength reveal an oscillatory decay and a semilinear
decay with distance from the interface in vacuum and water, respectively
Molecular Structure of Canonical Liquid Crystal Interfaces
Numerous
applications of liquid crystals rely on control of molecular
orientation at an interface. However, little is known about the precise
molecular structure of such interfaces. In this work, synchrotron
X-ray reflectivity measurements, accompanied by large-scale atomistic
molecular dynamics simulations, are used for the first time to reconstruct
the air-liquid crystal interface of a nematic material, namely, 4-pentyl-4′-cyanobiphenyl
(5CB). The results are compared to those for 4-octyl-4′-cyanobiphenyl
(8CB) which, in addition to adopting isotropic and nematic states,
can also form a smectic phase. Our findings indicate that the air
interface imprints a highly ordered structure into the material; such
a local structure then propagates well into the bulk of the liquid
crystal, particularly for nematic and smectic phases
Gelatin-Derived Graphene–Silicate Hybrid Materials Are Biocompatible and Synergistically Promote BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells
Graphene-based
materials are used in many fields but have found only limited applications
in biomedicine, including bone tissue engineering. Here, we demonstrate
that novel hybrid materials consisting of gelatin-derived graphene
and silicate nanosheets of Laponite (GL) are biocompatible and promote
osteogenic differentiation of mesenchymal stem cells (MSCs). Homogeneous
cell attachment, long-term proliferation, and osteogenic differentiation
of MSCs on a GL-scaffold were confirmed using optical microscopy and
scanning electron microscopy. GL-powders made by pulverizing the GL-scaffold
were shown to promote bone morphogenetic protein (BMP9)-induced osteogenic
differentiation. GL-powders increased the alkaline phosphatase (ALP)
activity in immortalized mouse embryonic fibroblasts but decreased
the ALP activity in more-differentiated immortalized mouse adipose-derived
cells. Note, however, that GL-powders promoted BMP9-induced calcium
mineral deposits in both MSC lines, as assessed using qualitative
and quantitative alizarin red assays. Furthermore, the expression
of chondro-osteogenic regulator markers such as Runx2, Sox9, osteopontin, and osteocalcin was upregulated by the GL-powder, independent of BMP9 stimulation; although the powder synergistically upregulated the BMP9-induced Osterix expression, the adipogenic marker PPARγ was unaffected. Furthermore, in vivo stem cell implantation experiments demonstrated that GL-powder could significantly enhance the BMP9-induced ectopic bone formation from MSCs. Collectively, our results strongly suggest that the GL hybrid materials promote BMP9-induced osteogenic differentiation of MSCs and hold promise for the development of bone tissue engineering platforms