Additional file 1: of Use of monomeric and oligomeric flavanols in the dietary management of patients with type 2 diabetes mellitus and microalbuminuria (FLAVA trial): study protocol for a randomized controlled trial

SPIRIT 2013 Checklist. (DOC 121 kb

Quantitative PCR validation.

<p>Comparison of fold changes between IFNγ/IL1β-stimulated untreated (n = 5) and myelin treated macrophages (n = 5). Relative quantification of gene expression (SCD1/2, ABCA1/G1 and RXRα/β/γ) was accomplished by using the comparative C_t method. Data were normalized to the most stable reference genes, determined by Genorm (18S and PGK1).</p

Myelin alters the macrophage phenotype by activating the LXRβ isoform.

<p>(a–d) Relative NO and IL-6 concentration in supernatants of IFNγ/IL-1β or LPS stimulated myelin- or T0901317-treated macrophages. The relative NO and IL-6 production is defined as the production of NO/IL-6 in experimental cultures divided by values in stimulated control cultures (dotted line). Data represent the mean of four independent experiments. (e, f) Relative NO and IL-6 concentration in supernatants of LPS stimulated myelin treated wild-type, LXRα-, LXRβ- and LXRαβ-deficient mouse macrophages. Macrophages were treated for 24 hours with myelin prior to stimulation with LPS. Data represent the mean of four independent experiments. Mye; Myelin: T09; T0901317.</p

Mye-macrophages have an increased capacity to transfer intracellular cholesterol towards HDL.

<p>Macrophages were loaded for 48 hours with 1,2- [3H] cholesterol after which cells were treated with myelin for 24 hours or left untreated. HDL was used as cholesterol acceptor. The relative cholesterol efflux is defined as the amount of transported cholesterol in culture medium of mye-macrophages divided by values in control macrophage cultures. Data represent the mean of four independent experiments.</p

Overrepresented canonical pathways and biological functions (IPA).

<p>Overrepresented canonical pathways and biological functions (IPA).</p

Top 20 up- and downregulated genes in mye-macrophages.

<p>Top 20 up- and downregulated genes in mye-macrophages.</p