34 research outputs found
Enhancing Central Nervous System Remyelination in Multiple Sclerosis
Recent studies on adult neural stem cells and the developmental biology of myelination have generated the expectation that neural precursors can repair the damaged central nervous system of multiple sclerosis patients where the endogenous remyelination process has failed. As a result, many laboratories are engaged in translational studies in which the goal is to design ways to promote remyelination and repair. Here we raise issues highlighted by prior experimental and human work that should be considered lest these studies become “lost in translation.
Neuregulin signaling regulates neural precursor growth and the generation of oligodendrocytes in vitro
Peer reviewe
Infectious Diseases of the Nervous System and Their Impact in Developing Countries
published_or_final_versio
Structural basis of hemadsorbing sites during the formation of syncytia in measles-infected cells
The surfaces of cells Infected with measles virus adsorb monkey red blood cells (RBC). In this study,
the structure and localization of viral hemadsorbing (HAD) sites and their relationship with antigenic
sites and with cell fusion were investigated in measles virus infected Vero cells. Transmission and
scanning electron microscopy were combined with immunolabeling, using human anti-measles IgG
(Ab) and protein A from Staph, aureus coupled to peroxidase.. In the early syncytia (50-100 2 in
diameter), HAD sites were clustered in the center but scattered at the periphery. In contrast, mature
giant cells (400 to 500 2) had all HAD sites in the central area which displayed scattered villi. The
periphery of the mature giant cells and the mononucleated cells did not hemadsorb but were covered
with numerous villi. In the central area, RBC were firmly attached to villi and ridges over nucleocapsids
but rarely to viral buds. Villi and ridges under the RBC were covered with antigenic sites which were
not detected at the periphery of the mature syncytia. When living cells were treated with Ab at 4°C,
complete inhibition of hemadsorption was only observed when RBC were applied to the cells in the
cold. In other experiments, cells reacted with Ab at 4°C were washed, brought to 37°C and treated
with RBC immediately or after 1-24 hrs. After 1-2 hrs, some HAD sites were present only at the
periphery of immature giant cells, suggesting that RBC receptors had already emerged in membrane
areas where fusion started again. After 24 hrs, the distribution of HAD and antigenic sites was the
same as on cells not exposed to Ab. It seems that when fusion begins, HAD sites appeared on the
periphery of the syncytia and then move spontaneously towards the center. With cessation of fusion,
HAD sites disappear from the periphery of the giant cell. Receptors for RBC are closely associated
with antigenic sites and correlated with viral induced fusion but not with virus production
ADRENOLEUCODYSTROPHIE LIEE A L'X (MECANISMES ET TRANSFERT DE GENE DANS LE SYSTEME NERVEUX CENTRAL)
PARIS-BIUSJ-Physique recherche (751052113) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocSudocFranceF
Growth and fate of PSA-NCAM+ precursors of the postnatal brain
Oligodendrocyte-type 2 astrocyte (O-2A) lineage cells are derived from multipotential stem cells of the developing CNS. Precursors of O-2A progenitors express the polysialylated (PSA) form of the neural cell adhesion molecule (NCAM) and are detected in neonatal rat brain glial cultures. It is unclear how such PSA-NCAM � “pre-progenitors ” are related to neural stem cells and whether they still have the potential to differentiate along several neural lineages. Here we isolated PSA-NCAM� pre-progenitor cells from glial cultures by immunopanning and found that most of these cells expressed nestin and PDGFreceptor-� but not O-2A antigens. PSA-NCAM � cells synthesized transcripts for fibroblast growth factor (FGF) receptors 1, 2, and 3 and responded to FGF2 by survival and proliferation, growing into large clusters resembling neural spheres. FGF2induced proliferation of PSA-NCAM � pre-progenitors was significantl
Assembly of enveloped RNA viruses - Chapter 7 Assembly of Coronaviridæ
This book is a collection of critical reviews about a diverse group of virus families with two features in common: the stable repository of genetic information in each virus is RNA, and each virus modifies and appropriates a particular patch of the eukaryotic cell membrane system to complete its structure. The reviews take the reader from the level of virus genome structure and expression through the quaternary interactions between virus-specified elements and cellular components that cooperate to produce virus particles. There are spectacular illustrations in this volume, but it is much more than a picture gallery. Reading widely in this book can be an effective antidote to overspecialization: in these pages, you are likely to learn much about viruses and about cells that you didn't know before; you'll discover illuminating parallels between diverse virus families; you'll come away with a sharpened awareness of important things that are still to be learned. Memphis, Tenn. , Summer 1984 David W. Kingsbury Preface This book was written at the suggestion of Dr. David W. Kingsbury made at a work shop on viruses organized by the Multiple Sclerosis Society in Aspen, Colorado, U. S. A. , three years ago. Originally, we had thought to focus on the morphological aspects of viral assembly. Later, during our discussions on the process of budding of enveloped RNA viruses, it became evident that we should include biochemical data in our review and correlate them with the structural aspects of virus maturation