Cytomegalovirus induces HLA-class-II-restricted alloreactivity in an acute myeloid leukemia cell line - Fig 4

<p>A: Alloreactivity-mediated proliferation of indolamin-2,3-dioxygenase-treated HCMV-infected AML cells co-cultured with mixed lymphocyte cultures. HCMV-infected AML cells co-cultured with mixed lymphocyte cultures from healthy controls were performed without and with 1 μM and 10 μM indolamin-2,3-dioxygenase (IDO). Panel (A) shows proliferative responses determined by H3 thymidine uptake (n = 3 experiments) and proliferative responses are presented as counts per minute (CPM). B: IFN-γ secretion by indolamin-2,3-dioxygenase-treated HCMV-infected AML cells co-cultured with mixed lymphocyte cultures. Panel (B) displays IFN-γ secretion determined by ELISpot, ELISpot results are given as spots per well. One-way-ANOVA (Friedman test) indicates that the groups were significantly different (p = 0.008) in terms of IFN-γ secretion.</p

Effect of CpG and ganciclovir on proliferation of HCMV-infected leukemia cells.

<p>Kasumi-1 or NB4 cells without (w/o) and with HCMV infection were co-cultured with PBMC without and with CpG-rich ODNs and/or ganciclovir and cell proliferation was measured by H3 thymidine uptake. The addition of CpG-rich ODNs to the co-cultures resulted in significantly higher proliferation of Kasumi-1 cells with vs. without CMV infection (panel A). Ganciclovir and the combination of CpG-rich ODNs and ganciclovir led to significantly increased proliferation in HCMV infected NB4 cells (panel B). Proliferative responses are given as counts per minute (CPM). Kasumi-1 or NB4 cells without and with HCMV infection were compared by Wilcoxon matched pairs test.</p