Dendrogram of resistant <i>E. coli</i> isolates’ PFGE profiles.

<p>Generated by cluster analysis of the Dice similarity indices in the BioNumerics fingerprinting software (optimization 1%, band matching tolerance 1%, tolerance change 1%). Isolates marked with “cip” were obtained by cultivation on ciprofloxacin and harbored PMQR genes, isolates with “ctx” represent the CTX-M-15 producing <i>E. coli</i>. “H” isolate from human, “D” isolate from dog, “C” isolate from cat.</p

Characteristics of ESBL-producing isolates from villages.

<p>All isolates were obtained on MCA-cefotaxime.</p><p>“D” – isolate from dog, “H” – isolates from human, “C” – isolate from cat. “T” in brackets means that the resistance gene was successfully transformed into competent cells, “C” in brackets means that the gene was conjugated. Plasmids were isolated and characterized from isolates in bold font. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113548#pone-0113548-t002" target="_blank">Table 2</a> for plasmid characteristics.</p><p>PG = phylogroup, Amp = ampicillin, Cef = cephalotin, Caz = ceftazidime, Amc = amoxycilin-clavulanate, Nal = nalidixic acid, Cip = ciprofloxacin, Tet = tetracycline, Sxt = trimethoprim-sulfamethoxazole, Spt = streptomycin, Sul = sulfonamides compounds, Gen = gentamicin, Chl = chloramphenicol, Cpd = cefpodoxime.</p><p>Characteristics of ESBL-producing isolates from villages.</p

Characteristics of isolates with PMQR genes.

<p>Isolates were obtained on MCA-ciprofloxacin.</p><p>For legend see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113548#pone-0113548-t001" target="_blank">table 1</a>.</p><p>Characteristics of isolates with PMQR genes.</p

Characteristics of plasmids obtained by transformation.

<p>“NT”–not typable. Note: transconjugants obtained in this study contained more than one plasmid and therefore were not used for plasmid characterization.</p><p>Characteristics of plasmids obtained by transformation.</p