Outcome of challenge with SP Taiwan19F-14 wt in RrgB321 immunized and control animals.

<p>Density of colonization in nasopharynx (a) and middle ear fluid (b) in RrgB321 immunized and control chinchillas challenged with SP Taiwan19F-14 wt (pilus +).</p

Evaluation of pilus expression on surface of <i>Streptococcus pneumoniae</i>.

<p>Flow cytometry on SP Taiwan19F-14 wt and its derivatives (ΔPI-1/PI-2 mutant and pMU1328_<i>Pc-rlrA</i> + strain) performed with pAb against RrgB. The percentage of pilus positive and negative bacteria for each strain was reported.</p

Proportion of strains predicted by MATS to be covered containing one or multiple vaccine antigens and strains not covered, within each clonal complex.

<p>Proportion of strains predicted by MATS to be covered containing one or multiple vaccine antigens and strains not covered, within each clonal complex.</p

Pilus expression of SP Taiwan19F-14 wt in nasal and middle ear washes of RrgB321 immunized and control animals.

<p>Flow cytometry analysis of Pilus-1 expression in nasal wash (NW) [A] and middle ear Fluid (MEF) [B] in RrgB321 immunized and control animals following challenge with SP Taiwan19F-14 wt.</p

Distribution of the vaccine antigens within each clonal complex.

<p>Distribution of the vaccine antigens within each clonal complex.</p

Pilus expression of SP Taiwan19F-14 wt in nasal and middle ear washes compared to inoculum.

<p>Flow cytometry analysis of Pilus-1 expression in nasal wash (NW) [A] and middle ear Fluid (MEF) [B] following challenge with SP Taiwan19F-14 wt.</p

Percentage of strain coverage predicted by MATS by age group.

<p>Percentage of strain coverage predicted by MATS by age group.</p

Number of invasive B stains isolated in Portugal from the first July 2011 to 30^th June 2015, and 4CMenB strain coverage predicted by MATS.

<p>Number of invasive B stains isolated in Portugal from the first July 2011 to 30^th June 2015, and 4CMenB strain coverage predicted by MATS.</p

Percentage of Portuguese strains covered by each one of 4CMenB and combinations.

<p>Percentage of Portuguese strains covered by each one of 4CMenB and combinations.</p

Outcome of challenge with SP Pilus-1 overexpressing mutant in RrgB321 immunized animals.

<p>Density of colonization in middle ear fluid in RrgB321 immunized and control chinchillas challenged with SP Taiwan19F-14 pMU1328_<i>Pc-rlrA</i> (100% pilus +).</p