Additional file 1: of Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Effect of parental age and sex of newborns. (DOCX 87 kb

Additional file 2: of Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Distribution of global methylation fractions based on modifiable factors: mode of egg fertilization by LUMA (A) and LINE1 assay (B); oxygen tension by LUMA (C) and LINE1 assay (D); type of embryo transfer by LUMA (E) and LINE1 assay (F) and; embryo transfer day by LUMA (G) and LINE1 assay (H). (PPTX 385 kb

Progesterone induces surface expression of EMP2 in mouse endometrium in vivo

Mice were injected with progesterone or sesame oil (control) daily for up to 5 days. Tissue was harvested at days 1, 2, and 4 of treatment or 3 days following completion of treatment, processed for immunohistochemistry, and then stained for EMP2 expression (A-D) or a vehicle control (E). (A) 24 hours of progesterone treatment; (B) 48 hours of treatment; (C) 4 days of treatment; (D) 3 days following treatment; (E) representative control treatment at 4 days. Magnification: (left) 100×; (right) 400×. The experiment was repeated at least 3 times with similar results; representative images are shown.<p><b>Copyright information:</b></p><p>Taken from "Steroid hormone regulation of EMP2 expression and localization in the endometrium"</p><p>Reproductive biology and endocrinology : RB&E 2008;6():15-15.</p><p>Published online 9 Apr 2008</p><p>PMCID:PMC2329639.</p><p></p

(A) Normal human endometrium specimens from subjects in proliferative and secretory phases were immunoblotted using EMP2 antisera

β-actin was used as a loading control. (B) Statistical analysis of EMP2 expression levels in proliferative (n = 3: subphase unspecified) and secretory (n = 3: one post-ovulatory day 3, one late, one unspecified) endometrial specimens from six independent patients. Data were analyzed using a 2-tailed, unpaired student t-test with a 95% confidence interval. *Changes in expression between the proliferative and secretory endometrium were significant, p = 0.038.<p><b>Copyright information:</b></p><p>Taken from "Steroid hormone regulation of EMP2 expression and localization in the endometrium"</p><p>http://www.rbej.com/content/6/1/15</p><p>Reproductive biology and endocrinology : RB&E 2008;6():15-15.</p><p>Published online 9 Apr 2008</p><p>PMCID:PMC2329639.</p><p></p