Mannose Binding Lectin Levels Was Not Associated with Resistance to Tuberculosis Infection in the Population of Uyo Metropolis in Nigeria

Mannose-binding lectin (MBL2) is an important pattern recognition molecule that identifies and binds to specific sugar molecules on the surface of pathogens thereby activating its destruction by the immune system. Samples for study were recruited from Uyo metropolis of Akwa Ibom state in Nigeria. In this study, levels of MBL2 was measured by enzyme-linked immunosorbent assay in tuberculosis patients and healthy individuals to determine if the immune protein protects against tuberculosis infection. MBL2 levels in tuberculosis patients and healthy controls were 14.0ng/ml ± 13.9 and 19.9ng/ml ± 18.5 respectively. The results from the study showed that there was no association in MBL2 levels between tuberculosis and controls (p=0.107) as well as between the different sub-groups. Therefore, MBL2 is not a contributory factor in resistance against tuberculosis in the population under study. Keywords: Mannose binding lectin, tuberculosis, pattern recognition molecule, immune system DOI: 10.7176/JNSR/12-14-02 Publication date:July 31st 202

Role of Some Metal Ions on Steady-state Kinetics of Engineered Wild-type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3)

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Vitamin B12 Supplementation: Effects On Some Biochemical And Haematological Indices Of Rats On Phenytoin Administration

Phenytoin is known to have some toxicological implications. Vitamin B12 supplementation during phenytoin administration was investigated to assess the benefits and risks of single vitamin supplementation. This study evaluated the biochemical and haematological effects of vitamin B12 on phenytoin toxicity. Twenty-four experimental animals were divided into 3 groups of 8 rats each. The control (group 1) received distilled water as placebo. Groups 2 and 3 were given 5mg/kg body weight of phenytoin for 4 weeks while group 3 in addition to phenytoin received intra-peritoneal administration of 15\u3bcg/kg body of vitamin B12 twice a week. Biochemical parameters such as AST, ALT, ALP, lipid profile and haematological indices were assayed as indices of toxicity. The result of the study showed that phenytoin administration resulted in anaemia which was ameliorated by vitamin B12 co-administration. Phenytoin also increased significantly the leukocyte count upon which B12 had no effect. Liver enzymes activities were significantly (p<0.05) raised during phenytoin administration and interestingly B12 further increased the level of these enzymes. Administration of phenytoin only gave a significant (p<0.05) increase in the level of serum Low density lipoprotein cholesterol. Serum cholesterol, TG and HDL-chol were not significantly affected. Although there was no significant change in serum cholesterol, the slight increase was more than 1% which is capable of causing a 3% increase in the risk of coronary heart disease. A significant decrease was also noted when phenytoin was supplemented with B12. We observed that vitamin B12 co-administration is beneficial in remitting anaemia and the atherosclerotic risk caused by phenytoin but may enhance hepatotoxicity. By this result we would therefore suggest that the use of vitamin B12 alone as supplement during phenytoin administration be discouraged