Advanced Techniques for Tooth Pulp RNA Extraction in Forensic Investigations-A Systematic Review

Teeth are fundamental structures in a forensic context due to their high strength and specificity. It is challenging to obtain high quality RNA from pulpal tissues. It has been described that pulp RNA degradation could be an indicator of post-mortem interval. Researchers have been in the quest to develop optimal techniques for tooth RNA extraction. The systematic review aimed to determine the most reliable method of RNA extraction from dental tooth pulp for forensic investigations

An Immunohistochemical Panel for The Accurate Differentiation Between Mucoepidermoid Carcinoma and Pleomorphic Adenoma

Salivary Gland Tumors (SGTs) are a diverse group of neoplasms, with a wide range of histological subtypes, some of which can exhibit overlapping features. Amongst this group of tumors, Pleomorphic Adenoma (PA) and Mucoepidermoid Carcinoma (MEC) stand out as prevalent entities originating from the salivary glands. The accurate differentiation between these two entities is crucial for treatment planning, as it influences decisions regarding the extent of surgical resection. Because of the overlapping histopathological features, it is difficult to differentiate these two lesions with the help of conventional Hematoxylin & Eosin (H& E). The evaluation of Immunohistochemical (IHC) markers holds pivotal importance in diagnosing salivary gland tumors, offering valuable insights into their molecular and cellular characteristics. The application of IHC markers assumes paramount importance in the precise diagnosis of MEC and PA within the context of salivary gland tumors. The study aimed to assess the effectiveness of immunobiomarkers in differentiation between mucoepidermoid carcinoma and pleomorphic adenoma

Immunohistochemical Expression of Cyclin D1 In Dentigerous Cyst, Odontogenic Keratocyst and Unicystic Ameloblastoma- An In-vitro Study

Cyclin D1, a G1 cyclin member, regulates the transition of the cell cycle from the G1 phase to the S phase. The uncontrolled and elevated expression of cyclin D1 has been associated with rapid growth, heightened proliferative activity and increased histologic aggressiveness. Unicystic ameloblastoma pertains to cystic lesions that present clinical, radiographic, or gross features akin to jaw cysts. Nevertheless, upon histological scrutiny, these lesions display a distinctive ameloblastomatous epithelium lining the cystic cavity, with or without luminal or mural proliferation. This cystic variant of tumor bears substantial resemblances to dentigerous cysts, and occasionally, both entities can become indistinguishable upon histological examination. The study aimed to investigate whether the immunohistochemical expression of cyclin D1 in unicystic ameloblastoma, odontogenic keratocyst and dentigerous cyst correlates with their known clinical behavior

Assessment of Micrometastasis in Lymph Nodes of Oral Squamous Cell Carcinoma –Attaining Excellence through Immunohistochemistry

Cervical lymph node metastasis is the most important prognostic factor in Squamous Cell Carcinoma of Head and Neck (SCCHN). Lymph node prognostic determinants hold immense significance in influencing both patient’s survival outcomes and the propensity for recurrence. The current study aimed to evaluate the diagnostic accuracy of Cyclin D1 and Pan-CK for the detection of lymph node micrometastasis in oral squamous cell carcinoma tissue sections and re-evaluate the tumor staging

Pathogenic Implications of Candidal Species in Both Oral Cancer and Oral Potentially Malignant Disorders - A Review

Oral squamous cell carcinoma (OSCC) is the 6th most common cancer in worldwide. Cigarette smoking, alcohol consumption, and betel quid chewing are its major risk factors other factors that can lead to OSCC include excessive sun exposure, viral or fungal infection, poor nutrition, and poor oral hygiene. The carcinogenesis of OSCC occurs gradually and oral epithelial dysplasia is considered a potential histologic precursor of OSCC. Most OSCC cases are preceded by clinically evident oral potentially malignant disorders (OPMDs). The aim of this review is to correlate the association between Candida species and oral potentially malignant disorders and its possible mechanisms in oral cancer progression