Identification of key microRNAs regulating ELOVL6 and glioblastoma tumorigenesis

ELOVL fatty acid elongase 6 (ELOVL6) controls cellular fatty acid (FA) composition by catalyzing the elongation of palmitate (C16:0) to stearate (C18:0) and palmitoleate (C16:1n-7) to vaccinate (C18:1n-7). Although the transcriptional regulation of ELOVL6 has been well studied, the post-transcriptional regulation of ELOVL6 is not fully understood. Therefore, this study aims to evaluate the role of microRNAs (miRNAs) in regulating human ELOVL6. Bioinformatic analysis identified five putative miRNAs: miR-135b-5p, miR-135a-5p, miR-125a-5p, miR-125b-5p, and miR-22–3p, which potentially bind ELOVL6 3′-untranslated region (UTR). Results from dual-luciferase assays revealed that these miRNAs downregulate ELOVL6 by directly interacting with the 3′-UTR of ELOVL6 mRNA. Moreover, miR-135b-5p and miR-135a-5p suppress cell proliferation and migration in glioblastoma multiforme cells by inhibiting ELOVL6 at the mRNA and protein levels. Taken together, our results provide novel regulatory mechanisms for ELOVL6 at the post-transcriptional level and identify potential candidates for the treatment of patients with glioblastoma multiforme

Experimental Challenges of the First Mass Measurement Campaign at the Rare-RI Ring

With the recent commissioning of the Rare-RI Ring (R3), nuclear mass measurement of rare isotopes (RIs) produced at the RI Beam Factory (RIBF) at RIKEN has become possible. The R3 spectrometer is based on the Isochronous Mass Spectrometry technique that allows for reaching a mass measurement precision of 10−6 within less than 1 ms. With the newly established self-triggered individual injection method, R3 specialized in mass measurements of extremely short-lived nuclei with low production yields. In this paper, we report the first mass measurement campaign conducted at the R3 addressing nuclei in the vicinity of N=50 and N=82 neutron magic numbers, with a particular focus on the challenges of this new facility