Figure_4B.csv

Ultrafiltration (molecular weight cut-off 50 kDa) of 0.1 μg/ml fluorescein-labelled high-molecular-weight hyaluronan (HMW-HA, ~800 kDa, Carbosynth) incubated overnight with 100 μg/ml transmembrane protein 2 ectodomain (sTMEM2) or sperm hyaluronidase (HAase, Sigma-Aldrich, H3884). The reaction buffer was 100 mM MES, pH 6.0, 1 mM CaCl2. The mock condition contained an irrelevant protein at 100 μg/ml (Endo180 domains 1-4).</p

Figure_4A.csv

Size exclusion chromatography of 100 μg/ml fluorescein-labelled high-molecular-weight hyaluronan (HMW-HA, ~800 kDa, Carbosynth) or hyaluronan 22-mer (dp22, ~5 kDa, Iduron), and of 100 μg/ml fluorescein-labelled HMW-HA incubated overnight with 100 μg/ml transmembrane protein 2 ectodomain (sTMEM2) or sperm hyaluronidase (HAase, Sigma-Aldrich, H3884). The reaction buffer was 100 mM MES, pH 6.0, 1 mM CaCl2.</p