15 research outputs found
Hemopoietic stem cell mobilization in mice
In mice hemopoietic stem cells and progenitor cells are
almost totally confined to the bone marrow and spleen. Only
small numbers can be detected in the peripheral blood.
Relatively little is known about the mechanism(s) modulating
the circulation and mobilization of stem cells. At present
their function and ultimate fate are unknown. Mobilization
of hemopoietic stem cells in mice has been achieved by
injection of a variety of substances, the most well known
being LPS. For some substances, including endotoxin, the
transient increase in blood CFU-s immediately following
injection (early mobilization) is followed by a second one
some days later (delayed mobilization).
The purpose of the investigations presented in this
thesis was to get insight into the mechanism(s) underlying
both early and delayed mobilization of hemopoietic stem
cells by LPS and other agents. Additionally, the changes in
splenic hemopoiesis seen after administration of LPS have
been subject of investigatio
Complement Split Product C5a Mediates the LipopolysaccharideâInduced Mobilization of CfuâS and Haemopoietic Progenitor Cells, But Not the Mobilization Induced By Proteolytic Enzymes
Abstract. Intravenous (i.v.) injection of mice with lipopolysaccharide (LPS), and the proteolytic enzymes trypsin and proteinase, mobilizes pluripotent haemopoietic stem cells (CFUâs) as well as granulocyteâmacrophage progenitor cells (GMâCFU) and the early progenitors of the erythroid lineage (EâBFU) from the haemopoietic tissues into the peripheral blood. We investigated the involvement of the complement (C) system in this process. It appeared that the early mobilization induced by LPS and other activators of the alternative complement pathway, such as Listeria monocytogenes (Lm) and zymosan, but not that induced by the proteolytic enzymes, was absent in C5âdeficient mice. the mobilization by C activators in these mice could be restored by injection of C5âsufficient serum, suggesting a critical role for C5. The manner in which C5 was involved in the C activationâmediated stem cell mobilization was studied using a serum transfer system. C5âsufficient serum, activated in vitro by incubation with Lm and subsequently liberated from the bacteria, caused mobilization in both C5âsufficient and C5âdeficient mice. C5âdeficient serum was not able to do so. the resistance of the mobilizing principle to heat treatment (56°C, 30 min) strongly suggests that it is identical with the C5 split product C5a, or an in vivo derivative of C5a. This conclusion was reinforced by the observation that a single injection of purified rat C5a into C5âdeficient mice also induced mobilization of CFUâs. Copyrigh
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