Evaluation of Pulp Tissue Following Direct Pulp Capping with Propolis versus Calcium Hydroxide: A Clinical Trial

Objectives Direct pulp capping may result in formation of a dentinal bridge and preservation of pulp vitality. This randomized controlled clinical trial sought to histologically assess and compare pulp tissue following pulp capping with propolis and calcium hydroxide. Methods In A cavity was prepared at the center of the occlusal surface of 10 third molars scheduled for extraction by using a cylindrical bur. The pulp chamber was exposed with a round bur. Samples were randomly divided into two groups (5 teeth in each group). The first group underwent direct pulp capping with propolis and the second group with calcium hydroxide. Auto-polymerizing glass ionomer was then applied to seal the cavity. The teeth were extracted after 45 days, and histologically evaluated. The obtained data were analyzed using the Fisher’s exact test. Results The quality (P=0.048) and quantity (P=0.008) of dentinal bridge were significantly different between the two groups. Propolis resulted in formation of a continuous dentinal bridge with irregular tubular dentin; whereas, calcium hydroxide resulted in formation of osteodentin (low quality dentin). Conclusion Propolis induced the formation of tubular dentin with higher quality compared with calcium hydroxide

Conservative Management of Periapical Cementoblastoma: A Case Report

As a rare ectomesenchymal neoplasm, benign cementoblastoma comprises less than 6% of all odontogenic tumors. The typical treatment plan involves surgical excision and extraction of the affected tooth. Limited evidence, however, suggests the conservative management of this condition as the best treatment. This article discusses the case of a 32-year-old man who had benign cementoblastoma and underwent conservative treatment. The diagnosis was established based on clinical and radiological features analyses. Root canal therapy was performed on the tooth, followed by enucleation, curettage, apicoectomy, and guided bone regeneration (GBR) 30 days later. After a year of follow-up, there was no recurrence, and the tooth was in healthy conditions. These findings demonstrated that the tooth affected by cementoblastoma can be saved. It was treated conservatively to preserve the patient’s oral health and masticatory function

Comparative Evaluation of the Viability of L929 Murine Fibroblasts in the Presence of Different Concentrations of Propolis with and without Vitamin C as a Storage Medium for Avulsed Teeth

Introduction: This study aimed to assess the optimal concentration of propolis with and without vitamin C as a storage medium for avulsed teeth Methods: Following the preparation of L929 murine fibroblasts suspension, 5,000 cells were seeded to each well of a 96-well plate. After 24 h, the culture medium was replaced with 0.01, 0.005, 0.001, 0.0005, 0.0001, and 0.00005 concentrations of propolis(P) and propolis plus vitamin C(PC) using Dulbecco’s Modified Eagle Medium. After 2, 24, and 72 h of incubation, the percentage of cell viability was determined by methyl thiazolyl tetrazolium assay, compared to the negative control group. Data were analyzed using the SPSS software (version 21). Two-way ANOVA was used to compare the means, while Tukey’s test was applied for pairwise comparisons.  Results: After 2 h, only the difference between the 0.001 concentration of P and PC was significant (P<0.005), such that cell viability was higher in the latter group. After 24 h, cell viability in 0.0005 and 0.00005 concentrations of P was significantly higher than that in the PC group. However, no significant difference was noted after 72 h. Conclusion: Cell viability was retained in all concentrations of propolis with or without vitamin C. On the other hand, with an increase in the concentration of propolis, cell viability decreased. Although PC was superior to propolis alone in cell viability; however, this effect decreased over time such that no significant difference was noted after 72 h

Photodynamic Therapy in Root Canal Disinfection: A Case Series and Mini-Review: Photodynamic Therapy in Root Canal Disinfection

Introduction: Photodynamic therapy (PDT) is now a widely used treatment modality in many fields of dentistry, including endodontics. The most common application of PDT in endodontics is to disinfect root canals. The purpose of this study was to present the experience of using PDT in root canal disinfection of three patients.Case Presentation: Three patients were referred to the Endodontics Department of Shahid Beheshti University of Medical Sciences and were treated using 0.1 mg/mL Toluidine Blue (FotoSan® agent; CMS Dental, Denmark) irradiated with a light-emitting diode (LED) lamp (FotoSan®; CMS Dental, Denmark) with a mean wavelength of 630 nm and a mean power density of 3 W/cm2 over two 30-second periods.Conclusion: PDT using LED lighting can be used in conjunction with conventional root canal treatment (RCT) to achieve great results. Doi:10.34172/jlms.2022.1

Synthesis of a Calcium Silicate Cement Containing a Calcinated Strontium Silicate Phase

Objectives. The positive effects of strontium on dental and skeletal remineralization have been confirmed in the literature. This study aimed to assess the properties of a calcium silicate cement (CSC) containing a sintered strontium silicate phase. Materials and Methods. The calcium silicate and strontium silicate phases were synthesized by the sol–gel technique. Strontium silicate powder in 0 (CSC), 10 (CSC/10Sr), 20 (CSC/20Sr), and 30 (CSC/30Sr) weight percentages was mixed with calcium silicate powder. Calcium chloride was used in the liquid phase. X-ray diffraction (XRD) of specimens was conducted before and after hydration. The setting time and compressive strength were assessed at 1 and 7 days after setting. The set discs of the aforementioned groups were immersed in the simulated body fluid (SBF) for 1 and 7 days. The ion release profile was evaluated by inductively coupled plasma-optical emission spectrometry (ICP-OES). Biomineralization on the specimen surface was evaluated by scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). Data were analyzed by the Kolmogorov–Smirnov test, one-way and mixed ANOVA, Levene’s test, and LSD post hoc test (P 0.05). The setting time significantly decreased by an increase in percentage of strontium (P <0.05). Release of Ca and Si ions significantly decreased by an increase in percentage of strontium (P <0.05). SEM/EDS showed the formation of calcium phosphate deposits at 1 and 7 days. Conclusion. Incorporation of 10−30 wt% sintered strontium silicate phase as premixed in CSC can significantly decrease the setting time without compromising the compressive strength or biomineralization process of the cement