6 research outputs found
Expanding the Substrate Scope of N- and O-Methyltransferases from Plants for Chemoselective Alkylation
- Author
- Publication venue
- 'Royal College of Obstetricians & Gynaecologists (RCOG)'
- Publication date
- 08/09/2023
- Field of study
Methylation reactions are of significant interest when generating pharmaceutically active molecules and building blocks for other applications. Synthetic methylating reagents are often toxic and unselective due to their high reactivity. SâAdenosylâlâmethionine (SAM)âdependent methyltransferases (MTs) present a chemoselective and environmentally friendly alternative. The anthranilate NâMT from Ruta graveolens (RgANMT) is involved in acridone alkaloid biosynthesis, methylating anthranilate. Although it is known to methylate substrates only at the Nâposition, the closest relatives with respect to amino acid sequence similarities of over 60â% are OâMTs catalysing the methylation reaction of caffeate and derivatives containing only hydroxyl groups (CaOMTs). In this study, we investigated the substrate range of RgANMT and a CaOMT from Prunus persica (PpCaOMT) using compounds with both, an aminoâ and hydroxyl group (aminophenols) as possible methyl group acceptors. For both enzymes, the reaction was highly chemoselective. Furthermore, generating cofactor derivatives inâ
situ enabled the transfer of other alkyl chains onto the aminophenols, leading to an enlarged pool of products. Selected MT reactions were performed at a preparative biocatalytic scale in inâ
vitro and inâ
vivo experiments resulting in yields of up to 62â%
Measurement of the t(t)over-bar production cross section in pp collisions at root s=7 TeV in dilepton final states containing a tau
- Author
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- Adler Volker
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- Field of study
The top quark pair production cross section is measured in dilepton events with one electron or muon, and one hadronically decaying tau lepton from the decay t (t) over bar -> (l nu(l))((sic)(h)nu((sic)))b (b) over bar, (l = e, mu). The data sample corresponds to an integrated luminosity of 2.0 fb(-1) for the electron channel and 2.2 fb(-1) for the muon channel, collected by the CMS detector at the LHC. This is the first measurement of the t (t) over bar cross section explicitly including tau leptons in proton- proton collisions at root s = 7 TeV. The measured value sigma(t (t) over bar) = 143 +/- 14(stat) +/- 22(syst) +/- 3(lumi) pb is consistent with the standard model predictions
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Online parent-targeted cognitive-behavioural therapy intervention to improve quality of life in families of young cancer survivors: study protocol for a randomised controlled trial
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A Transdiagnostic Minority Stress Treatment Approach for Gay and Bisexual Menâs Syndemic Health Conditions
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