Geometric evaluation of the modeled protein and the template shown as Ramachandran plots for 4KOF (A) and modeled protein (B); 97% of residues were in the favor, 2.9% allowed regions, and 0.2% was in the outlier region as compared to 4KOF template (97.5%, 2.3%, and 0.2%).

<p>Geometric evaluation of the modeled protein and the template shown as Ramachandran plots for 4KOF (A) and modeled protein (B); 97% of residues were in the favor, 2.9% allowed regions, and 0.2% was in the outlier region as compared to 4KOF template (97.5%, 2.3%, and 0.2%).</p

Docking and binding mode of (A) FLC (B) E5 and (C) EUG in the active site pocket of modelled CYP51 of <i>Candida albicans</i>.

<p>EUG showing interaction with other residues of the protein away from the pocket and heme.</p

Isobolograms depicting synergistic interactions of eugenol-tosylates (E1 to E6) with fluconazole in nine different ratios against different <i>Candida albicans</i> isolates.

<p>Isobolograms depicting synergistic interactions of eugenol-tosylates (E1 to E6) with fluconazole in nine different ratios against different <i>Candida albicans</i> isolates.</p

UV spectrophotometric sterol profiles of representative laboratory strain <i>C</i>. <i>albicans</i> ATCC 90028 (A), clinical fluconazole-susceptible <i>C</i>. <i>albicans</i> 2281 (B) and Clinical fluconazole-resistant <i>C</i>. <i>albicans</i> 3001 (C) strains after treatment with MIC and ½ MIC values of compounds E1 to E6.

<p>Isolates were grown for 16 h in sabouraud dextrose broth and sterols were extracted using alcoholic KOH and n-heptane from the treated and untreated cells and spectral profiles between 240 and 300 nm were determined. Control represents the culture cells without any treatment and positive control represents cells treated with 64 μg/ml of fluconazole.</p

Relative expression of <i>ERG11</i> of <i>C</i>. <i>albicans</i> ATCC90028, <i>C</i>. <i>albicans</i> 2281 and <i>C</i>. <i>albicans</i> 3001 exposed to different eugenol-tosylates (E1-E6) or FLC at their respective MIC values.

<p>Control represent the compound free cells and had an average relative expression (of 3 independent recordings) with *3.124±0.124; **2.234±0.134; ***1.795±0.102</p