3 research outputs found
Genetic affinities of Fusarim spp. and their correlation with origin and pathogenicity
Random amplified polymorphic DNA (RAPD) analyses was used in combination with pathogenicity assays to study the taxonomic kinships among five Fusarium species. A total of 46 isolates of Fusarium spp. obtained from diseased cotton seedlings showing typical root rot and dampping-off symptoms were characterized. Of 10 primers tested, four primers produced polymorphic amplification patterns with taxon-specific bands, in addition to individual-specific bands. Genetic analysis indicated into 2 main clusters, with the minor cluster included all F. moniliforme and F. solani at the genetic similarity of GS=57.82%. The major cluster consisted of all F. oxysporum, F. avenaceum and F. chlamydosporum clustered at 71% similarity. There was no clear-cut relationship between clustering in the RAPD dendrogram, pathogenicity test and geographic origin of tested isolates. The results suggest that RAPD-PCR is a useful method for analysing genetic variation within and between Fusarium spp.
(African Journal of Biotechnology: 2003 2(5): 109-113
Comparison of multi-locus enzyme and protein gel electrophoresis in the discrimination of five Fusarium species isolated from Egyptian cottons
Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell
protein (SDS-PAGE) were carried out in order to evaluate the parity
between different methods for the characterization of five Fusarium
 species recovered from cotton-growing areas in Egypt by
numerical taxonomy methods. The obtained data revealed that SDS-PAGE
and esterase isozymes are more efficient in grouping isolates in their
respective species while peroxidase and malate dehydrogenase isozyme
has much limited resolution in organizing all isolates in their
respective species-specific clusters. A low correlations was detected
between geographical origin of isolates and genetic diversity. Results
indicate that the estimated inter-specific variation may be more
pronounced with protein markers than with isoyzmes when the two
approaches are applied to the same populations. The level of genetic
variability detected within and between Fusarium spp. accessions with
protein and esterase isoyzmes analysis suggests that it is a reliable,
efficient, and effective marker technology for determining genetic
relationships in Fusarium genus