Extra dose of Dsn1p (Yir010wp) expressed from episomal plasmid in normal Saccharomyces cerevisiae diploid wild type has augmented growth rate and altered budding pattern, suggesting its growth stimulating property

DSN1 is a spindle pole body component that functions in the chromosomal segregation process of yeast. The effect of additional DSN1 gene dosage on the yeast cell has yet to be established. The work from this study indicated that an extra gene dosage of DSN1 caused abnormalities in both cellular growth and budding pattern. The yeast cells carrying an additional copy of DSN1 gene have an increased growth rate and they formed a highly retarded multiple bud complex. These phenomena suggest possible growth stimulating property of the gene product

The identification and characterization of a novel transcription factor LeCBP, a potential regulator of the tomato stylar Chi2;1 promoter

A gene, LeCBP encoding a protein containing a PHD zinc finger motif and a bipartite nuclear localization signal, was identified from tomato pistils by its binding ability to a specific DNA motif using a yeast one-hybrid system. The LeCBP has the ability to bind specifically to a 20 bp region of the promoter of Chi2;1, encoding a pistil chitinase. LeCBP also has the ability to activate the yeast URA3 reporter gene in yeast. An analysis of the temporal and spatial patterns of gene expression through northern blotting and in situ hybridization has demonstrated the highest levels of expression in mature tomato pistils where the mRNA was localized along the transmitting tracks of the style as well as on the ovules and ovary walls. The specific binding property of LeCBP with Chi2;1 promoter may be used to engineer high levels of expression in pistils of transgenic plants. © 2004 Elsevier Ireland Ltd. All rights reserved

Isolation and characterization of a novel pistil predominant gene that binds weakly to the chitinase, Chi2;1 promoter of tomato

A gene LN2-1-1 identified by screening a tomato pistil cDNA library has been found to encode a basic protein containing ankryin repeats. This clone was isolated by its binding ability to a specific DNA fragment in the tomato floral chitinase promoter using a yeast one hybrid system. An analysis of the temporal and spatial patterns of gene expression through northern blotting has demonstrated the highest level of expression in mature tomato pistils. In situ hybridization revealed that the mRNA was maximal on the upper half of the style and decreased in a gradient from the top to the lower half of the style. Hybridization was also detected on the stigma, ovules and ovary walls. The LN2-1-1 could possibly play a role in modulating the defense mechanism of tomato flowers based on its ability to bind to the chitinase, Chi 2;1 promoter