Repellent activity of asaricin 1 on <i>Ae</i>. <i>Aegypti</i>, <i>Ae</i>. <i>albopictus</i> and <i>Cx</i>. <i>quinquefasciatus</i>.

<p>Repellent activity of asaricin 1 on <i>Ae</i>. <i>Aegypti</i>, <i>Ae</i>. <i>albopictus</i> and <i>Cx</i>. <i>quinquefasciatus</i>.</p

Preliminary larvicidal activity on fractions.

<p>All fractions (F1-F8) from roots hexane extracts tested on <i>Ae</i>. <i>aegypti</i>, <i>Ae</i>. <i>albopictus</i> and <i>Cx</i>. <i>quinquefasciatus</i> late third early fourth instar larvae.</p

¹H-NMR (400 MHz) and ¹³C-NMR (100 MHz) spectral data of <i>trans</i>-asarone 3 in CDCl₃.

<p>¹H-NMR (400 MHz) and ¹³C-NMR (100 MHz) spectral data of <i>trans</i>-asarone 3 in CDCl₃.</p

HMBC and COSY correlation of asaricin 1, isoasarone 2 and <i>trans</i>-asarone 3.

<p>HMBC and COSY correlation of asaricin 1, isoasarone 2 and <i>trans</i>-asarone 3.</p

Binding residues of three binding sites of AChE enzyme, blue, orange and lime green residues depict the anionic binding site (TRP83), catalytic binding site (SER238, GLU367, HIS480) and peripheral binding site (TRP271) respectively.

<p>Binding residues of three binding sites of AChE enzyme, blue, orange and lime green residues depict the anionic binding site (TRP83), catalytic binding site (SER238, GLU367, HIS480) and peripheral binding site (TRP271) respectively.</p

¹H-NMR (400 MHz) and ¹³C-NMR (100 MHz) spectral data of asaricin 1 in CDCl₃.

<p>¹H-NMR (400 MHz) and ¹³C-NMR (100 MHz) spectral data of asaricin 1 in CDCl₃.</p

Inhibition and Larvicidal Activity of Phenylpropanoids from <i>Piper sarmentosum</i> on Acetylcholinesterase against Mosquito Vectors and Their Binding Mode of Interaction - Fig 17

<p>Docking structures of compound (A) asaricin <b>1</b> and (B) isoasarone <b>2</b> toward Anionic site of AChE and their closed contact residue interaction. Residue interacted with compound at < -2 kcal/mol were shown only.</p

Isolation procedures of asaricin 1, isoasarone 2 and <i>trans</i>-asarone 3 as larvicide.

<p>The <i>P</i>. <i>sarmentosum</i> roots hexane extract was fractionated using hexane- dichloromethane. The preliminary test had identify the active fraction and relative active fraction (F2) were further purified using thin layer chromatography (TLC). Isolated asaricin <b>1</b>, isoasarone <b>2</b> and <i>trans</i>-asarone <b>3</b> were tested toward late third early fourth instar larvae from <i>Ae</i>. <i>aegypti</i>, <i>Ae</i>. <i>albopictus</i> and <i>Cx</i>. <i>quinquefasciatus</i>.</p

Chemical constituents; asaricin 1, isoasarone 2 and <i>trans</i>-asarone 3 which were isolated from roots of <i>P</i>. <i>sarmentosum</i>.

<p>Chemical constituents; asaricin 1, isoasarone 2 and <i>trans</i>-asarone 3 which were isolated from roots of <i>P</i>. <i>sarmentosum</i>.</p

The interaction energy of phenolic compounds toward AChE catalytic site.

<p>The interaction energy of phenolic compounds toward AChE catalytic site.</p