Serološka srodnost i antigenska prevlast između pet izolata virusa bolesti Aujeszkoga.

The serologic relationship and antigenic dominance among five Aujeszky’s disease virus (ADV) isolates, designated VBA1, VBA2, VBA3, mA1p and CD, was studied using homologous and heterologous antigens and sera. Cross-serum neutralization test (C-SNT) was used to measure antibody responses to the virus. Generally, close serologic relationships among ADV isolates were noted. Non-significant (P<0.05) variations in the relationship between any two viruses were observed. However, the highest bilateral relationship was observed between VBA2 and VBA3 and between VBA2 and CD isolates of the virus. The antigenic dominance of some viruses over the others was detected with non-significant (P<0.01) levels, although the higher antigenic dominance was observed for CD over VBA3 and mA1p over VBA3 also. In conclusion, neither major serological variations nor antigenic dominance among the five ADVs studied were detected in the present study, hence grouped as one serologic type.Upotrebom homolognih i heterolognih antigena i seruma istraživan je serološki odnos i antigenska prevlast između pet izolata virusa bolesti Aujeszkoga (VBA) označenih VBA1, VBA2, VBA3, mA1p i CD. Unakrižnim serum-neutralizacijskim testom mjerena je količina specifičnih protutijela. Općenito je zapažena jaka serološka srodnost između pretraženih izolata. Uočena su beznačajna odstupanja (P<0,05) u srodnosti bilo koja dva izolata. Međutim, najjača srodnost uočena je između VBA2 i VBA3 te između VBA2 i CD izolata. Ustanovljeno je da antigenska prevlast jednih izolata nad drugima nije bila značajna (P<0,01), iako je uočena nešto veća antigenska prevlast CD izolata nad VBA3 te mA1p nad VBA3. Može se zaključiti da u ovom radu nije zabilježeno ni značajnije serološko odstupanje niti antigenska prevlast između pet istraživanih izolata virusa bolesti Aujeszkoga pa su svrstani u jedan serološki tip

Serološka srodnost i antigenska prevlast između pet izolata virusa bolesti Aujeszkoga.

The serologic relationship and antigenic dominance among five Aujeszky’s disease virus (ADV) isolates, designated VBA1, VBA2, VBA3, mA1p and CD, was studied using homologous and heterologous antigens and sera. Cross-serum neutralization test (C-SNT) was used to measure antibody responses to the virus. Generally, close serologic relationships among ADV isolates were noted. Non-significant (P<0.05) variations in the relationship between any two viruses were observed. However, the highest bilateral relationship was observed between VBA2 and VBA3 and between VBA2 and CD isolates of the virus. The antigenic dominance of some viruses over the others was detected with non-significant (P<0.01) levels, although the higher antigenic dominance was observed for CD over VBA3 and mA1p over VBA3 also. In conclusion, neither major serological variations nor antigenic dominance among the five ADVs studied were detected in the present study, hence grouped as one serologic type.Upotrebom homolognih i heterolognih antigena i seruma istraživan je serološki odnos i antigenska prevlast između pet izolata virusa bolesti Aujeszkoga (VBA) označenih VBA1, VBA2, VBA3, mA1p i CD. Unakrižnim serum-neutralizacijskim testom mjerena je količina specifičnih protutijela. Općenito je zapažena jaka serološka srodnost između pretraženih izolata. Uočena su beznačajna odstupanja (P<0,05) u srodnosti bilo koja dva izolata. Međutim, najjača srodnost uočena je između VBA2 i VBA3 te između VBA2 i CD izolata. Ustanovljeno je da antigenska prevlast jednih izolata nad drugima nije bila značajna (P<0,01), iako je uočena nešto veća antigenska prevlast CD izolata nad VBA3 te mA1p nad VBA3. Može se zaključiti da u ovom radu nije zabilježeno ni značajnije serološko odstupanje niti antigenska prevlast između pet istraživanih izolata virusa bolesti Aujeszkoga pa su svrstani u jedan serološki tip

Comparison of the restriction enzyme profiles of herpesvirus isolates from captive wildlife

Herpesvirus has been commonly isolated from captive wildlife in animal sanctuaries. Other then identification of the virus, attempts to trace the origin of the virus were initiated based on DNA restriction enzymes (RE) analysis. Two isolates from gaurs (UPMV4/05, UPMV5/05) and an isolate from a Malayan sun bear (UPMV19/05) were examined for a homologous relationship among them. As expected, the DNA from UPMV4/05 and UPMV5/05 showed close RE similarity in comparison to UPMV19/05. The RE patterns from this herpesvirus isolates indicate a possible evolution from the same origin, bovine herpes virus (BHV). The present study showed the DNA of isolates UPMV4/05, UPMV5/05 and UPM19/05 to have an average molecular weight 0f 112x10⁶ Dalton

Adenoviral based gene therapy for cancer in human and animals: a review

Adenovirus vector is the most common used vector in clinical gene therapy. The development of adenovirus from the first generation until the helper-dependent adenovirus vector has greatly reduced toxicity and immunogenicity. The helper-dependent adenovirus can also prolong transgene expression. Tissue- or disease-specific approach has been used to improve the specificity of adenoviral vector for cancer gene therapy. This review summarizes some adenoviral gene therapy and targeting approaches available for human cancer as well as animal cancer