Characterization and functional study of stress-associated protein in rice and arabidopsis

Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morpho-physiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerance in plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity

Effect of rice AUXIN BINDING PROTEIN57 (OsABP57) overexpression in response to flooding

This study reports the effects of Oryza sativa AUXIN BINDING PROTEIN57 (OsABP57) overexpression towards flooding in rice. OsABP57 was previously reported to activate plasma membrane H+ -ATPase. Earlier studies address the ability of transgenic OsABP57 overexpression in enduring drought and salinity stresses but none on the flooding. In this study, complete submergence analysis was carried out and several morphophysiological parameters were analyzed such as plant height, root architecture and relative chlorophyll content. Results showed that there are no differences between OsABP57 overexpression rice compared to MR219 control rice in terms of chlorophyll content and plant height after 1-3 weeks of flooding treatments. Root analysis, however, found that transgenic rice OsABP57 produced more adventitious roots compared to MR219 rice under normal condition, which may be due to the role of the gene that encodes for auxin binding protein. The semi-quantitative polymerase chain reaction (PCR) on Oryza sativa pyruvate decarboxylase (OsPDC) gene after two weeks of flooding treatment showed an increase of expression in OsABP57 transgenic compared to MR219. Overall, the overexpression of OsABP57 did not show any significant difference in terms of morphophysiological analysis between the transgenic line and MR219, yet, there is an increase of OsPDC gene in the transgenic background which may need further experimental analysis in the future to map the network between auxin and hypoxia core genes