Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

The present study aimed to production of purified L-asparaginase from Vignaunguiculata. Different physiological parameters, such as pH, temperature and incubation period, were optimized for growth and maximum L-asparaginase production. The optimum parameters were 37°C, 30 min and pH 8.5. Maximum L-asparaginase was 886.4U/ml with a specific activity of 1140.7 U/ml (31 fold purification with 28 %yield) were obtained at optimum conditions. The purified L-asparaginase produced from Vignaunguiculata was used for characterization and general properties. The effect of pH and temperature on L-asparaginase activity as well as stability at different pH and temperature were determined. The optimum pH 8.5 and 37ºC temperature on L-asparaginase showed 100% residual activity. Stability of pH around 8.5 and temperature 70ºC showed 90 and 78 % residual activity at 30 and 60 min respectively. The L-asparaginase showed high stability at alkaline pH (pH 8.5) when incubated for up to 60h.The molecular weight of the produced L-asparaginase was close to 68.5 kDa. Cytotoxic activity of L-asparaginase was examined in vitro using four carcinoma cell lines. L-aspargenase has higher effective in growth inhibition against HEPG2 and HCT-116 but lower against HELLA and MCF7 carcinoma cell lines. The data show that L-aspargenase has a higher cytotoxic activity against HEPG2 and HCT116, revealed higher percentage of cell death, indicating antitumor properties, and demonstrate direct effect on cancer cell proliferation of HEPG2 and HCT116. Therefore, Vignaunguiculata was considered to be a suitable source for production of L-asparaginase has higher activity and good stability. Purified L-asparaginase obtained from Vignaunguiculata could be employed in drug chemotherapy and treatment of cancer

Efecto del tipo y nivel de fibra dietética suplementada en ratas

Both experimental and clinical studies have indicated that a novel source of dietary fibres is potentially hypolipidemic. In the present study, turnip, sugar beet, cabbage and fenugreek green leaves were used as natural new sources of dietary fibres, to examine their effects on nutritional parameters and lipid metabolism in rats fed for 8 weeks comparing with a control diet (fibre-free control). Chemical analyses of the 4 plants revealed that there are differences between the ratios of soluble and insoluble non - starch polysaccharides (NSP) and their monomers constituents. Higher levels of uronic acid were present in soluble fibre (NSP) in all fibre sources than that of insoluble one. Generally, highly significant decrease in the weight gain and food intake of the rats fed the 4 experimental diets after 8 weeks feeding comparing with those fed control diet. Rats fed turnip diet exhibited a highly significant decrease in all the nutritional parameters through the feeding period. Rats fed a diet supplemented with sugar beet fibre, have a highly significant decrease in the value of weight gain, food intake and Dapp over the feeding period. Sugar beet, cabbage and fenugreek green fibre-containing diets had lowering effect on serum total lipid, total cholesterol, LDL-C (except sugar beet) and triglycerides levels than that of control diet fed rats. Supplemented the diet with turnip fibre had only lowering effect on serum triglycerides over the feeding period. Hepatic total lipid and total cholesterol levels were significantly lower when rats fed cabbage and fenugreek green fiber-containing diets. Highly significant decrease in the level of triglycerides was achieved in the liver of rats fed turnip fibre supplemented diet comparing with the experimental period (8 weeks).Estudios clínicos y experimentales han indicado que nuevas fuentes de fibras dietéticas son potencialmente hipolipídicas. En el presente estudio nabo, remolacha, col y fenogreco verde fueron utilizados como nuevas fuentes naturales de fibra dietética, para examinar sus efectos en parámetros nutricionales y en el metabolismo lípidico de ratas alimentadas durante 8 semanas comparando con una dieta control (control-libre de fibra). El análisis químico de las 4 plantas reveló que hay diferencias entre las proporciones de polisacáridos no amiláceos insolubles y solubles (NSP) y sus constituyentes monómeros. Los niveles mas altos de ácidos urónicos se presentan en la fibra soluble (NSP) que en la insoluble, para todas las fuentes de fibra. Se observa una disminución altamente significativa en la ganancia de peso y en la ingesta de alimentos para las ratas alimentadas, con las 4 dietas experimentales, después de 8 semanas, comparado con la dieta control. Las ratas alimentadas con la dieta de nabo muestran una disminución altamente significativa en todos los parámetros nutricionales durante el periodo de alimentación. Las ratas alimentadas con una dieta suplementada con fibra de remolacha tienen una disminución altamente significativo en el valor de ganancia de peso, ingesta de alimento y Dapp durante el periodo de alimentación. Las dietas con contenido en fibra de remolacha, col y fenogreco verde tienen un efecto de disminución de los niveles en suero de lípido total, colesterol total, LDL-C (excepto remolacha) y triglicéridos sobre las ratas alimentadas con la dieta control. La dieta suplementada con fibra de nabo tiene solo disminuye los triglicéridos en suero durante el periodo de alimentación. Los niveles de lípido total y colesterol total en hígado fueron significativamente más bajos cuando las ratas se alimentan con dietas de col y fenugreco verde. Existe una disminución altamente significativa en el nivel de triglicéridos en el hígado de ratas alimentadas con dieta suplementada con fibra de nabo comparando con el periodo experimental (8 semanas)