Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822)

Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps

Not Available

Not AvailableFingerling rearing of pengba, Osteobrama belangeri, was conducted at varied densi-ties for 90 days in 12 outdoor concrete tanks (10 m × 5 m × 1 m). The tanks, grouped into four triplicated treatments, were stocked with fry at 20, 30, 40 and 50 fry m-3; and designated as control, T-1, T-2 and T-3 respectively. Tanks were filled up to 90 cm depth. Evaporation loss compensated fortnightly. Fish sampling and monitoring of water quality parameters done at fortnight intervals. Survival varied between 71.5% and 84.0% in treatments. Both survival and total length significantly reduced with increasing densities from control to T-2 (p .05). Similarly, significant reductions in final body weight and specific growth rate were observed with increased densities and these values further reduced in T-3 than T-2 (p < .05). Such results corroborated the inverse relationship between stocking density and growth. Gross biomass yields in two higher densities (T-2 and T-3), despite their lower survivals, were significantly higher than the two lower densities. The numbers of fingerlings harvested were 35, 74 and 112% higher than the control in T-1, T-2 and T-3 respectively. The lowest size obtained even at the highest density (T-3) was of 7.0 ± 1.0 cm and 3.99 ± 0.17 g, which can be considered as ideal grow-out stocking size of pengba. Therefore, the study showed feasibility of stocking up to 50 fry m-3 for rearing from fry to fingerling stage. However, the other lower densities may be used in case of requirement of larger fingerlings.Not Availabl

Not Available

Not AvailableSerum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purifed by affnity chromatography using BSA-CL agarose column. The purifed mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purifed m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purifed m-Ig indicated that, all the three MAbs were specifc to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps.Not Availabl