Additional file 4: of Hypothermia for encephalopathy in low and middle-income countries (HELIX): study protocol for a randomised controlled trial

SPIRIT checklist. (PDF 77 kb

Primer sequences for polymerase chain reactions.

<p>Primer sequences for polymerase chain reactions.</p

Proportion of G6PD enzyme activity levels for male and female participants compared to adjusted male median value.

<p>For each G6PD enzyme activity level (U/g Hb) shown in X-axis, the corresponding value in Y-axis indicates the number of participants. The numbers on top of each dotted line, shown as 10, 20, 30, and 60 on uppermost horizontal line of the graph indicate different cut-off values as percentages for the study population. 60% (shown as 60) of adjusted male median (shown as 100) is the upper limit of cut-off value and the participants with enzyme activities below 60% are considered deficient. Black portion of each bar indicates male participants, whereas gray portion of each bar indicates female participants.</p

Hemoglobin levels and reticulocyte counts in G6PD non-deficient (ND) and deficient (D) participants.

<p>(A) Depicts hemoglobin levels (g/dL), and (B) Demonstrates reticulocyte counts (%) in non-deficient and deficient participants. A p-value < 0.05 was considered statistically significant.</p

Fluorescent spot test and enzyme assay results of deficient samples along with reticulocytes counts.

<p>Fluorescent spot test and enzyme assay results of deficient samples along with reticulocytes counts.</p

Distribution of demographic information between non-deficient and deficient participants.

<p>Distribution of demographic information between non-deficient and deficient participants.</p

Reference values to describe the G6PD activity profile for study population.

<p>Reference values to describe the G6PD activity profile for study population.</p