Using Diode Laser for Soft Tissue Incision of Oral Cavity

INTRODUCTION: The purpose of this case report is to analyze the results of vestibuleplasty around an over-denture based on dental implants to determine the evidence on the effectiveness of laser-assisted surgery and soft tissue management in implant dentistry.CASE REPORT: The patient in this case report was a 57 year- old male who was completely edentulous, with a mandibular over denture based on 4 standard implants suffering from pain on chewing, and a general discomfort, especially in anterior segment. He received a standard gingival augmentation surgery which failed to create sufficient vestibular depth. A laser-assisted vestibuloplasty was done to make the environment suitable for making a new overdenture.CONCLUSIONS: There were clinically significant improvements in vestibular depth and peri implant soft tissue situation by laser. Also, bleeding during surgery was well controlled; the patient’s discomfort and pain were less than the first operation done with gingival graft. Based on our clinical data and review of the literature, an evidence-based approach to the use of lasers for the management of soft tissue in oral surgery is necessary. Simply, there is insufficient evidence to suggest that any specific wavelength of laser is superior to the traditional modalities of therapy. However, improved hemostasis and patient’s satisfaction can be considered as advantages of adjunctive laser therapy in some clinical situations like vestibuloplasty

Histologic and Histomorphometric Evaluation of Maxillary Sinus Floor Elevation Using Nanobone® and Easy-Grafttm Crystal: A Split-Mouth Clinical Trial

Maxillary sinus floor elevation is an effective method for bone augmentation in the posterior maxilla. Due to the limitations of autogenous bone grafts, bone substitutes are often used for this purpose. This study sought to compare the histologic and histomorphometric results of using NanoBone® and easy-graft™CRYSTAL for maxillary sinus floor elevation. This randomized double-blind split-mouth clinical trial was conducted on nine healthy patients requiring bilateral (n=18) sinus floor augmentation. Dental implants were placed six months after sinus floor elevation. Biopsy samples were taken at the time of implant surgery and analyzed using HistoMorphoMeter Ver.1.0 software. Histomorphometric analysis indicated that NanoBone® and easy-graft™ residues accounted for 32.71±10.39% and 26.61±9.48% of the bioptical volume, respectively. The amount of new bone formation was 25.29±7.29% and 18.69±5.63% in the NanoBone® and easy-graft™ groups, respectively. Paired samples t-test showed significant differences between the two groups in this respect (P=0.0001). Wellmineralized regenerated bone with lamellar parallel-fibered structure and Haversian systems surrounded the particles in both groups. Both tested materials yielded acceptable histological outcomes six months after surgery. NanoBone® caused superior new bone formation. Although longer follow-ups and larger sample size are needed, these preliminary results encourage further research in this respect

The potential of human-derived periodontal ligament stem cells to osteogenic differentiation: An In vitro investigation

Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0.01 μM), and their proliferation was measured. The osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity and Alizarin Red-S staining for calcium deposition. Results: After the administration of 0.01 μM Dex, the activity of ALP increased significantly. Furthermore, mineralized nodule formation showing the intracellular calcium deposition was significantly higher in the Dex-treated cells than that of the control cells. Conclusion: Collectively, Dex has positive effects on osteogenic differentiation of human PDLSCs in vitro. It is suggested that PDLSCs may serve as a potential material for periodontal tissue regeneration