Description of SNPs present in Wnt pathway genes that were analyzed.

*<p>Source – dbSNP, National Center for Biotechnology Information.</p

Comparison of genotype frequencies of SNPs in Wnt pathway genes between ER- breast cancers versus controls.

<p>BC. Breast Cancer; ER-. Estrogen Receptor negative; OR 95% CI. Odds Ratio and 95% Confidence Interval.</p>*<p>P<0.05 was considered significant and are depicted in bold.</p

Genotype frequencies of Wnt pathway gene polymorphism in breast cancers and Controls.

<p>BC. Breast Cancer; OR 95% CI. Odds Ratio and 95% Confidence Interval.</p>*<p>P<0.05 was considered significant and are depicted in bold.</p

Comparison of genotype frequencies of SNPs in Wnt pathway genes between early on-set Breast cancers (patients age ≤43 years) versus controls.

<p>BC. Breast Cancer; OR 95% CI. Odds Ratio and 95% Confidence Interval.</p>*<p>P<0.05 was considered significant and are depicted in bold.</p

Expression and Polymorphism of Toll-Like Receptor 4 and Effect on NF-κB Mediated Inflammation in Colon Cancer Patients

<div><p>Our aim was to evaluate the association between the expression and the polymorphism of TLR4/NF-κB pathways and colon cancer. TLR4 (<i>rs4986790</i>, <i>rs10759932</i>, <i>rs10759931</i> and <i>rs2770150</i>) were genotyped in blood samples from Colorectal patients and healthy controls. TLR4 and cytokines inflammatory expression were evaluated by real time PCR on 40 matching normal and colon tissues and the protein level by Immunohistochemistry. The high level of TLR4 expression in colon cancer tissues is mainly due to infections by bacteria in the human colon and leads to induction of an acute secretion of inflammatory cytokines mediated by NF-κB. Also, we report here a clear evidence for an association between TLR4 <i>rs10759931</i> polymorphism (OR = 0.086, CI: 0.04–0.18, P = <0.00001). This polymorphism affects the entire population without being specific to either gender or to any age group. In contrast, the <i>rs2770150</i> is associated with colon cancer in women aged over 50 years and is closely linked with the decreased levels of female sex hormones during the post-menopausal period (OR = 0.188, CI: 0.074–0.48, P = <0.00084). <i>rs10759932</i> and <i>rs4986790</i> appear to have any association with colon cancer. Our data suggest that TLR4 SNPs could possibly serve as biomarkers for decision making in colon cancer treatment.</p></div

Inflammatory cytokines expression in colon cancer tissues compared to normal matching tissues:

<p>Cytokine production was assessed by qRT-PCR on colonic cancer tissues and matching normal tissues (mean ± SD, n = 40 patients, data normalized to <i>GAPDH</i>, control (open bars), cancer (solid bars), *P<0.00 t-test)</p