ZEBOV trafficking involves early and late endosomes.

<p>(<b>A</b>) <b>ZEBO-VLPs colocalize with vesicles bearing early endosomal antigen-1 (EEA1) shortly after internalization.</b> HEK293T cells were incubated with fluorescently-labeled ZEBO-VLPs (green) for 10 min at 16°C. After washing to remove unbound VLPs, fresh growth medium was added to cells, which were then incubated at 37°C. At the indicated time cells were fixed, permeabilized and stained for EEA1 (red). Nuclei (blue) were stained with DAPI. Images were taken by confocal microscopy using a 100× oil immersion objective lens. A representative image of mid-optical z-section is shown for each time point. (<b>B</b>) <b>Quantitation of VLP colocalization.</b> VLPs colocalized with EEA1 were counted and expressed as percent of total VLPs in image sections. At least 10 images (5–6 cells/image) were analyzed for each sample. Mean ± st.dev. are presented in the data. (<b>C</b>) <b>ZEBOV requires Rab5 and Rab7 function.</b> HEK293T cells were made to express GFP or GFP-tagged forms of DN Rab5, DN Rab7 by plasmid transfection. Twenty-four h post-transfection cells were incubated with wild-type ZEBOV for 48 h. Cells were fixed after 36 h and immunostained for ZEBOV VP40 matrix protein as a marker of infection. Nuclei were stained with DAPI and images were taken by fluorescence microscopy. Image analysis was performed using Cell Profiler software (Broad Inst. MA) as described in methods. The proportion of cells that were expressing each GFP-tagged fusion protein and infected by ZEBOV was calculated as a fraction of the total cell population and averaged for all replicates (>5). Data were normalized to that seen in cells transfected with GFP alone. (<b>D</b>) <b>Rab5 and Rab7 function is necessary for the cell entry step of infection.</b> To determine the step of infection that was affected by each DN protein, entry assays were performed using HEK293Tcells expressing GFP, or GFP-tagged forms of wild-type Rab5, DN Rab5 or DN Rab7. Cells were incubated with VSV-VLP (open bars) or ZEBO-VLP (solid bars) for 3 h. Subsequently, luciferase activity was measured in each sample and expressed relative to that in control (untransfected) cells. The data represents average ± st.dev. of 3 independent experiments, each performed in duplicate.</p

Cholesterol-enriched lipid raft microdomains are important for ZEBOV entry.

<p>(<b>A</b>) <b>ZEBO-VLPs associate with lipid rafts.</b> Vero cells were incubated with gfpZEBO-VLP (green) at 37°C for 15 min and unbound virus was removed by washing. Lipid rafts were visualized by first incubating the cells with Alexafluor₅₉₄-labeled CTxB (red) followed by coalescing the small raft domains with anti-CTxB antibody. The samples were then fixed and images taken by confocal microscopy. A mid z-section of the cells is shown. Insets i and ii are enlarged images of the indicated areas. (<b>B</b>) <b>Cholesterol sequestering drugs inhibit ZEBOV infection.</b> Vero cells were pretreated with the indicated concentrations of methyl-β cyclodextrin or nystatin for 1 h. Cells were then washed extensively to remove the drugs, and gfpZEBOV was added at an MOI of 0.1. After 24 h, cells were washed and fixed. Images were then taken with a 10× objective lens. The number of foci of infected (gfp-expressing) cells were counted for 4 images per sample in duplicate. The average number of foci is indicated ± st.dev. Similar results were obtained with HEK293T cells (not shown).</p

Clathrin and caveolar endocytosis are not required for ZEBOV entry.

<p>(<b>A</b>) <b>Regulators of clathrin and caveolae-mediated endocytosis are not important for ZEBOV infection.</b> The role of proteins important for endocytosis in ZEBOV infection was assessed using dominant negative (DN) effector proteins. HEK293T cells were transfected with plasmids encoding GFP, DN-Eps15-GFP or DN-Cav1-GFP. Twenty-four h post-transfection cells were inoculated with wild-type ZEBOV (MOI  = 0.2). Cells were fixed 48 h later, stained for nuclei using DAPI and for ZEBOV VP40 matrix protein using a specific rabbit antiserum followed by Alexa₆₃₃ secondary antibody. Images were taken by fluorescence microscopy and analyzed as described in the methods. To quantitate the infection dependency of ZEBOV on expression of each construct, the proportion of cells that were expressing each GFP-tagged fusion protein and infected by ZEBOV was calculated as a fraction of the total cell population. The data were averaged for all replicates (>5) and normalized to that seen in cells transfected with GFP alone. (<b>B</b>) To measure the impact of expression of each GFP-tagged protein on the virus entry step into cells, a contents mixing assay was performed. Cells were transfected as above and then used in the assay 36 h after transfection. Both ZEBO-VLPs and VSV-G pseudotyped particles were used as indicated. Measurements were made at 3 h, at which time the contents mixing signal peaked in untreated cells (peak is at 2–3 h post cell binding). Measurements were normalized to untransfected cells. The results are mean ± st.dev. of 3 independent experiments. (<b>C</b>) To test DN-Cav1 efficacy, HEK293 cells were transfected with plasmids encoding GFP or DN-Cav1 tagged with GFP. Thirty six hours after transfection cells were infected with a recombinant 10A1 MLV virus encoding a truncated CD4 receptor as a marker for infection. 36 h after the infection cells were stained for CD4 expression with anti-CD4 antibody conjugated to PE (red) and cells expressing CD4 and the GFP-tagged protein by microscopy. Data were analyzed as described in the methods and in (A). (<b>D</b>) <b>Cholera toxin B subunit uptake is blocked in cells expressing DN-Cav-1.</b> As an additional test of DN-Cav-1 efficacy, the impact of expression on cholera toxin subunit B (CTxB) uptake was measured. HEK293T cells were transfected with plasmid encoding GFP (left panel) or GFP-tagged DN-Cav1 protein (right panel). Thirty-six h after transfection cells were incubated with fluorescently-labeled CTxB for 30 or 60 min, fixed and imaged. Images were taken by confocal microscopy with a mid z-section shown. Green  =  GFP or DN-Cav1; Red  =  CTxB. (<b>E</b>) <b>ZEBO-VLPs do not associate with markers of caveolae or clathrin-coated endosomes.</b> Vero cells were preincubated with gfpZEBO-VLPs at 16°C (to prevent endocytosis) for 15 min to allow virus attachment. Excess virus was then removed and the temperature raised to 37°C (to initiate endocytosis) prior to fixation at indicated times. For caveolin-1 and clathrin light chain A, permeabilized cells were stained with anti-Cav1 antibody or anti-CLCA antibody followed by Alexafluor₅₉₄-conjugated secondary antibody. For transferrin, Alexafluor₅₉₄-labeled transferrin was added to cells during incubation with the VLPs. DAPI was used to stain nuclei (blue). Images were taken by confocal microscopy with a mid z-section shown. Green  =  gfpZEBO-VLPs; Red  =  indicated endocytic marker.</p

Actin and actin regulatory proteins are important for ZEBOV infection.

<p>(<b>A</b>) <b>Suppression of Pak1 by siRNA blocks ZEBOV infection.</b> HEK293 cells were transfected with siRNA targeting Pak1 (two distinct siRNA, i and ii, used) or non-targeting siRNA. Expression of Pak1 was evaluated by Western blot using an appropriate antibody (Cell Signaling Technology, MA) and relative peak intensity determined by densitometry using a Typhoon scanner and associated software (GE Biosciences, NJ). The impact of Pak1 suppression on gfpZEBOV infection was then determined and expressed relative to untransfected controls. (<b>B</b>) <b>DN Pak1 reduces ZEBOV infection.</b> HEK293T cells were transfected with plasmids encoding β-galactosidase (β-gal) or myc/GST-tagged forms of wt Pak1 or DN-Pak1. 36 h later, cells were infected with gfpZEBOV and after 24 h were fixed and stained for myc or GST tags using appropriate primary and secondary antibodies. Cells were then imaged and analyzed as in the methods. The proportion of cells that were expressing each tagged protein and infected by ZEBOV was calculated as a fraction of the total cell population and expressed relative to the infection seen for cells transfected with plasmid encoding β-galactosidase. (<b>C</b>) <b>Suppression of CtBP/BARS by siRNA blocks ZEBOV infection.</b> HEK293 cells were transfected with siRNA targeting CtBP/BARS (two used, i and ii) or non-targeting or firefly luciferase (luc) targeting siRNA. Expression levels were determined by evaluating immunofluorescent staining intensity of CtBP/BARS in nuclei of each cell (CtBP/BARS is predominantly localized to cell nucleus) and normalizing to the nuclear stain, DAPI and untransfected controls. The left panel shows portion of microscope image with cell nuclei stained with DAPI or CtBP/BARS antibody and center panel shows quantitation of staining from 20,000 cells. Right panel shows impact on infection by ZEBOV-GFP. (<b>D</b>) <b>ZEBOV induces Arp2-nucleation.</b> Vero cells were incubated in medium without virus or replication-competent infectious ZEBOV (MOI  = 5) for the indicated time. Subsequently cells were washed, fixed, permeabilized and stained for Arp2 protein using a specific antibody. The number and apparent size of Arp2 complexes was analyzed using the Analyze particles function of ImageJ software (<a href="http://rsbweb.nih.gov/ij/" target="_blank">http://rsbweb.nih.gov/ij/</a>). While total number of Arp2 clusters did not change, the size distribution was altered by ZEBOV incubation with cells. This was expressed as the number of Arp2 complexes of the size ranges indicated (area occupied in image) relative to the total number of complexes (*-P<0.05, **-P<0.01). (<b>E</b>) Images showing Arp2 nucleation. Arp2 (red), DAPI stained nuclei (blue). Images were taken by confocal microscopy using a 100× oil immersion objective lens. (<b>F</b>) <b>ZEBO-VLPs associate with Arp2 complexes.</b> Vero cells were incubated with gfpZEBO-VLPs (green) for 30 min and then fixed, permeabilized and stained for Arp2 (red) using appropriate antibodies. (<b>G</b>) <b>ZEBO-VLPs associate with actin foci and</b> (<b>H</b>) <b>VASP protein during cell entry.</b> Vero cells were incubated with fluorescently-labeled ZEBO-VLPs or VSV-VLPs (green). After 30 min, cells were washed, fixed and permeabilized. For actin staining, cells were incubated with medium containing fluorescently-labeled phalloidin (red). For VASP staining, cells were incubated with anti-phospho-VASP antibody, followed by fluorescently-labeled secondary antibody (red). Arrowheads indicate representative examples of VLP colocalization with actin or VASP. All Images were taken by confocal microscopy using a 100× objective lens.</p

ZEBOV uptake and infection is inhibited by EIPA and VLP uptake is associated with dextran containing vesicles.

<p>(<b>A</b>) <b>EIPA inhibits dextran accumulation into vesicles.</b> Vero cells were treated with DMSO or EIPA (50 uM) for 30 min. Subsequently, cells were incubated with Alexafluor₅₉₄-labeled dextran (1 mg/ml) in the presence of the inhibitor. After 30 min, cells were washed, fixed and observed by confocal microscopy. Nuclei (blue) were stained with DAPI. Images were taken using a 100× oil immersion objective lens. (<b>B</b>) Accumulation of dextran in cells was analyzed by counting the total number of macropinocytic vesicles (occupying >0.25 µm² in images) relative to the area occupied by the cell. (<b>C</b>) <b>EIPA blocks ZEBOV infection.</b> Vero cells were pre-treated with the indicated concentrations of EIPA, followed by incubation with gfpZEBOV (top panel) or rfpVSV (middle panel) each at MOI of 0.1 in the continued presence of the drug. Control cells received DMSO instead of the drug. After 24 h, cells were washed and fixed. Virus infection was determined by counting fluorescent foci. Cell monolayer integrity was confirmed by phase-contrast microscopy (bottom panel). (<b>D</b>) Quantitation of data shown in (C). Solid bars represent gfpZEBOV and open bars represent rfpVSV. Data were normalized to the average number of foci seen for untreated cells. Similar results were obtained when HEK293T cells were used (not shown). (<b>E</b>) <b>EIPA-mediated block is at the entry step of infection.</b> The mechanism of the EIPA-mediated inhibition of infection was examined by performing entry assays. HEK293T cells were pre-treated with the indicated concentrations of EIPA for 1 h followed by incubation with ZEBO-VLP (solid bars) or VSV-VLP (open bars) for an additional 3 h in the continued presence of the drug. Subsequently, cells were washed, and luciferase activity was measured for each sample. The results are expressed as luciferase activity relative to that in control (DMSO-treated) cells. The data represents average ± st.dev. of 3 independent experiments each performed in duplicate. (<b>F</b>) <b>EIPA does not affect ZEBO-VLP binding to cells.</b> HEK293 cells were pre-treated with EIPA (50 µM) for 1 h at 37°C, followed by incubation with ZEBO-VLPs for 10 min at room temperature. Cells were then washed to remove unbound virus, resuspended in luciferase assay buffer containing triton X-100 detergent, and luciferase activity was measured. Data were normalized to luciferase activity in vehicle-treated samples. Each data point represents mean ± st.dev. of 3 experiments. (<b>G</b>) <b>EIPA treatment inhibits cellular uptake of ZEBO-VLPs.</b> Vero cells were treated with DMSO or EIPA (50 µM) for 30 min. Subsequently, cells were incubated with gfpZEBO-VLP (green) in the presence of the inhibitor. After 30 min, cells were washed, fixed and the cell periphery was visualized by staining with phalloidin (red), staining cortical actin. Images were taken by confocal microscopy using 100× oil immersion objective lens. Only the mid-optical section representing the cell interior is shown. (<b>H</b>) VLP uptake was quantified by counting the total number of internalized VLPs in cells in each image (4–6 cells/image). A total of 10 images were analyzed for each sample. The data are presented as average number of VLPs per cell ± st.dev. (<b>I</b>) <b>Internalized ZEBO-VLPs colocalize with dextran.</b> HEK293T cells were incubated with gfpZEBO-VLP at 16°C. After 15 min, samples were washed and incubated with Alexafluor₅₉₄-labeled dextran 10,000 W (1 mg/ml) at 37°C. At indicated time intervals, cells were fixed and analyzed by confocal microscopy. Each image represents a mid optical section. Arrowheads indicate examples of association between gfpZEBO-VLPs (green) with dextran-containing vesicles (red). Nuclei (blue) were stained with DAPI. <b>Quantitation of VLP colocalization</b> (right panel). Multiple sections of each image were analyzed for VLPs that exhibited colocalization with dextran and their number expressed as percent of total VLPs in those sections. At least 10 images (5–6 cells/image) were analyzed for each sample. Mean ± st.dev. are shown. Note: this is likely an underestimate of the association as VLPs out of the plane or close to but not completely overlapping dextran positive vesicles were not counted. (<b>J</b>) <b>ZEBOV induces dextran uptake by cells.</b> Vero cells were incubated with replication-competent VSV or ZEBOV (MOI  = 5) for 15 min. Control cells were incubated with growth medium alone (none). Cells were then washed and incubated with medium containing fluorescently-labeled dextran 10,000 MW (1 mg/ml). After 30 min, cells were washed and fixed. Images were then taken by confocal microscopy using a 100× oil immersion objective lens, and the number of dextran-containing vesicles in individual cells were counted. For each sample, at least 10 images (≥25 cells) representing randomly selected fields were analyzed. The data represent mean ± st.dev. of dextran-containing vesicles/cell. A similar outcome was observed when HEK293T cells were incubated with ZEBO-VLP (not shown).</p

Validation of luciferase virus entry assay for ZEBOV pseudotypes and VLPs.

<p>(A) The luciferase entry assay was performed using EVP (open bars) or VSVP (solid bars) or ZEBO-VLP (diagonally hatched bars) on HEK293-mCAT-1 cells in the presence of anti-Ebola virus neutralizing antibody (KZ52, 0.3 µg/ml) or a control antibody of irrelevant specificity (anti-HA, 12CA5; Roche, 0.3 µg/ml). Data were normalized to luciferase activity in cells incubated with untreated virus. Each data point represents the mean±SD of 3 independent experiments. (B) Luciferase entry assays were performed using VSVP, EVP, or FrVP on HEK293-mCAT-1 cells in the presence of either 20 mM ammonium chloride (open bars) or 50 nM bafilomycin A1 (solid bars). Effect of ammonium chloride was also tested on ZEBO-VLP. Data were normalized to luciferase activity for untreated cells. Each data point represents the mean±SD of 3 independent experiments. (C) Kinetics of entry was measured for EVP (open squares), VSVP (closed squares), and ZEBO-VLP (open circles). Each was incubated with HEK293-mCAT1 cells at room temperature. After 10 min, cells were washed to remove unbound virus and incubated at 37°C. At indicated time intervals, aliquots of cells were withdrawn, and luciferase signals were measured. For each virus, luciferase activity at different time points was normalized to the maximum luciferase activity for that virus. Each data point represents the mean±SD of 2 independent experiments.</p

PI3K-Akt-1 pathway plays a critical role in Ebola virus entry.

<p>(A) HEK293-mCAT-1 cells were pre-treated with the indicated concentrations of LY294002 for 1 h followed by incubation with ZEBO-VLP (open squares), VSV-VLP (closed squares), EVP (open circle), or VSVP (closed circle) for 3 h in the continued presence of the drug. Entry signals were then measured. Data were normalized to luciferase activity in DMSO (vehicle)-treated cells. (B) HEK293-mCAT-1 cells were pre-treated with the indicated concentrations of Akt inhibitor for 1 h followed by incubation with ZEBO-VLP (open squares), VSV-VLP (closed squares), EVP (open circle), or VSVP (closed circle) for 3 h in the continued presence of the drug. Entry signals were then measured. Data were normalized to luciferase activity in DMSO (vehicle)-treated cells. (C) HEK293-mCAT-1 cells were transfected with either pcDNA3 or pcDNA3 encoding a dominant-negative form of the PI3K p85-α regulatory subunit (Δp85α). Entry assays were performed 36 h after transfection using ZEBO-VLP (open bars) or VSV-VLP (solid bars). Data were normalized to luciferase activity in untransfected cells. Each data point represents the mean±SD of 3 experiments. (D) Wild-type (WT), pik3R1^−/− (p85α, p55α, and p50α-deficient), or pik3R2^−/− (p85β -deficient) mouse embryonic fibroblast (MEF) cells were incubated with EVP (open bars) or FrVP (solid bars). After 3 h, cells were washed, and luciferase activity was measured. Each data point represents the mean±SD of 3 independent experiments. (E) HEK293-mCAT-1 cells were pre-treated with LY294002 (50 µM) or Akt-1 inhibitor (1.0 µM) for 1 h at 37°C, followed by incubation with ZEBO-VLPs for 10 min at room temperature. Cells were then washed to remove unbound virus and resuspended in luciferase assay buffer containing triton X-100 detergent; luciferase activity was then measured. Data were normalized to luciferase activity in vehicle-treated samples. Each data point represents the mean±SD of 2 experiments.</p

Activity of luciferase-containing pseudotyped virus and virus-like particles in the entry assay.

<p>ND, not determined; VLPs do not encode an expressed transgene to allow titer determination.</p>a<p>Pseudotyped virus particles or VLPs were pelleted through sucrose and resuspended in 0.01 volumes of the original culture volume. A total of 0.2 ml of virus was used per assay with 10⁶ cells and values were measured after 3 h of incubation.</p>b<p>Virus was prepared as above and used to infect HEK293-mCAT-1 cells. Titer was determined by limiting dilution using GFP reporter expression to count infected cells. Values for titer and entry assay are the mean±SD of 3 independent experiments.</p

ZEBOV particles induce Akt-1 phosphorylation early during infection.

<p>Serum-starved HEK293 cells were incubated with serum-free medium (−), medium containing 10% FBS (S), and serum-free medium containing γ-radiation–inactivated ZEBOV (E, MOI = 5) or VSV (V, MOI = 5) at 37°C for 30 or 60 min. Subsequently, cells were washed and lysed, and phosphorylated Akt-1 was detected by Western blotting (p-Akt-1; top panel). The same membrane was then stripped and re-probed for total Akt-1 (bottom panel). The bar graph shows a summary of densitometry analysis of bands, which was performed using the ImageJ gel analysis package. The ratio of band intensities of phospho-Akt-1 to total Akt-1 was determined and normalized to the serum-treated control. Each data point represents mean±SD of 3 experiments.</p

PI3K is important for early step(s) during ZEBOV infection.

<p>Vero-E6 cells were incubated with LY294002 (50 µM) or Akt inhibitor (10 µM) for 2 h in the presence of virus. Unbound virus and drug was then removed and cells were cultivated. After 10 d, cells were fixed in formalin and stained with crystal violet; plaques were then counted. Effect of the drugs on VSV (Indiana strain) infection was also tested similarly, except that plaques were counted 2 d after inoculation of cells. Open bars, ZEBOV; solid bars, VSV (* <i>p</i><0.01).</p