Dual-Echo Magnetic Resonance Imaging Pulse Sequence in Diagnosis of Temporomandibular Disorders: Diagnostic Accuracy Study

Objective: This study was conducted to assess the diagnostic accuracy of different MRI pulse sequence dual-echo-PD in the diagnosis of temporomandibular disorders. Subjects and methods: Twenty seven patients (54 Temporomandibular joints) with painful joints or limited mouth opening or unexplained longstanding headache were enrolled in the study to examine the diagnostic accuracy of magnetic resonance imaging sequence dual-echo in delineation of the articular disc position, the disc morphology, and the cortical bone in comparison to the gold standard of MRI scanning sequence: proton density weighted images. Results: Dual-echo-PD weighted image showed 94.7% sensitivity and 96.3% diagnostic accuracy in identifying articular disc position. Dual-echo-PD showed no statistically significant difference than proton-density in delineation of disc morphology or cortical bone. Conclusions: The dual-echo imaging sequence can be used instead of using PD to delineate disc position, morphology, and cortical bone

Zinc Oxide and Silicone Nanoparticles to Improve the Resistance Mechanism and Annual Productivity of Salt-Stressed Mango Trees

Limited findings have been reported on using nanomaterials to improve tree fruit growth, development, and productivity under various stress conditions. To assess the effect of nanoparticles (NPs) like nano-zinc oxide (nZnO) and nano-silicon (nSi) on mango tree growth, yield, and fruit quality under salinity conditions, a factorial experiment was conducted using twelve treatments; three replicates each. Foliar spray of nZnO (50, 100, and 150 mg/L), nSi (150 and 300 mg/L), their combinations, and distilled water as a control was applied at full bloom and one month after of salt-stressed “Ewais” mango trees. Trees positively responded to different levels of nZnO and nSi. Plant growth, nutrients uptake, and carbon assimilation have improved with all treatments, except the higher concentration of nSi. Plant response to stress conditions was represented by a high level of proline content with all treatments, but changes in the activity of the antioxidant enzymes were positively related to the lower and medium concentrations of NPs. Flower malformation has significantly decreased, and the annual fruit yield and physiochemical characteristics have improved with all treatments. It could be recommended that a combination of 100 mg/L nZnO and 150 mg/L nSi improves mango tree resistance, annual crop load, and fruit quality under salinity conditions

The Anti-Rheumatic Drug, Leflunomide, Induces Nephrotoxicity in Mice via Upregulation of TGFβ-Mediated p53/Smad2/3 Signaling

Recent studies indicated renal toxicity and interstitial nephritis in patients receiving leflunomide (LEFN), but the exact mechanism is still unknown. The transforming growth factor β (TGFβ)/p53/Smad2/3 pathway crucially mediates renal fibrosis. We aimed to assess the nephrotoxic effect of LEFN in mice and the possible role of TGFβ-stimulated p53/SMAD2/3 signaling. The study design involved distributing sixty male albino mice into four groups: (i) vehicle-treated mice, (ii) LEFN (2.5 mg/kg), (iii) LEFN (5 mg/kg), and (iv) LEFN (10 mg/kg). The drug was given orally every 48 h and continued for 8 weeks. Blood samples were then taken from mice for the determination of kidney function parameters. Right kidneys were used for histopathologic staining and immunohistochemistry, whereas left kidneys were frozen and used for Western blot analysis of the target proteins, p-p53 and Smad2/3. Results indicated that chronic administration of LEFN in mice resulted in a four- and nine-fold increase in serum urea and creatinine levels, respectively. Kidney specimens stained with hematoxylin and eosin or periodic acid–Schiff showed significant histopathological manifestations, such as cellular irregularity, interstitial congestion, and moderate lymphocytic inflammatory infiltrate in mice treated with LEFN. Western blotting indicated upregulation of the p-p53/Smad2/3 proteins. LEFN, especially in the highest dose (10 mg/kg), produced prominent nephrotoxicity in mice. This toxicity is mediated through stimulating fibrotic changes through TGFβ-stimulated p53/Smad2/3 signaling and induction of glomerular and tubular apoptosis. An improved understanding of LEFN-induced nephrotoxicity would have great implications in the prediction, prevention, and management of leflunomide-treated rheumatic patients, and may warrant further clinical studies for following up these toxidromes

5-Aminolevulinic Acid and 24-Epibrassinolide Improve the Drought Stress Resilience and Productivity of Banana Plants

Plant growth, development, and productivity are adversely affected under drought conditions. Previous findings indicated that 5-aminolevulinic acid (ALA) and 24-epibrassinolide (EBL) play an important role in the plant response to adverse environmental conditions. This study demonstrated the role of ALA and EBL on oxidative stress and photosynthetic capacity of drought-stressed ‘Williams’ banana grown under the Egyptian semi-arid conditions. Exogenous application of either ALA or EBL at concentrations of 15, 30, and 45 mg·L−1 significantly restored plant photosynthetic activity and increased productivity under reduced irrigation; this was equivalent to 75% of the plant’s total water requirements. Both compounds significantly reduced drought-induced oxidative damages by increasing antioxidant enzyme activities (superoxide dismutase ‘SOD’, catalase ‘CAT’, and peroxidase ‘POD’) and preserving chloroplast structure. Lipid peroxidation, electrolyte loss and free non-radical H2O2 formation in the chloroplast were noticeably reduced compared to the control, but chlorophyll content and photosynthetic oxygen evolution were increased. Nutrient uptake, auxin and cytokinin levels were also improved with the reduced abscisic acid levels. The results indicated that ALA and EBL could reduce the accumulation of reactive oxygen species and maintain the stability of the chloroplast membrane structure under drought stress. This study suggests that the use of ALA or EBL at 30 mg·L−1 can promote the growth, productivity and fruit quality of drought-stressed banana plants