Pomegranate peel as a natural antioxidant boosts bucks' fertility under Egyptian summer conditions

[EN] Exposure of male rabbits to heat stress during summer adversely affects their fertility, leading to major production losses. A total of 16 male rabbits were randomly divided into 4 experimental groups exposed to temperatures ranging from a high of 30.3 to a low of 27.3 °C. Animals from first to fourth groups were fed diets containing 0, 1.5, 3.0, or 4.5% pomegranate peel (PP) to evaluate the ability of PP feeding to enhance bucks¿ reproductive status. Pomegranate peel treatments significantly increased ejaculate volume by 19, 18 and 12%, increased seminal plasma fructose by 7, 18, and 24%, improved sperm motility by 28, 34 and 49%, increased sperm total output by 37, 69 and 102% and reduced dead sperm by 24, 32 and 64% with diets containing 1.5, 3.0 and 4.5% of PP compared to the heat stressed control animals. Seminal plasma total lipids, cholesterol and triglycerides increased while alkaline phosphatase decreased with PP treatments. Seminal plasma total antioxidant capacity increased to reach 126, 143 and 191% with diets containing 1.5, 3.0 and 4.5% of PP, while lipid peroxide (malondialdehyde) levels decreased significantly to reach around 54% of the heat stressed bucks¿ value with the three PP dietary doses used. It was concluded that supplementations of PP in the diet of bucks during summer season in Egypt can improve their semen characteristics, probably due to their antioxidant actions.Zeweil, HS.; Elnagar, S.; Zahran, SM.; Ahmed, MH.; El-gindy, Y. (2013). Pomegranate peel as a natural antioxidant boosts bucks' fertility under Egyptian summer conditions. World Rabbit Science. 21(1):33-39. doi:10.4995/wrs.2013.1209.SWORD333921

Annona Muricata L. extract restores renal function, oxidative stress, immunohistochemical structure, and gene expression of TNF-α, IL-β1, and CYP2E1 in the kidney of DMBA-intoxicated rats

Introduction: 7,12-dimethylbenz (a) anthracene (DMBA) is a harmful polycyclic aromatic hydrocarbon derivative known for its cytotoxic, carcinogenic, and mutagenic effects in mammals and other species. Annona muricata, L. (Graviola; GRV) is a tropical fruit tree traditionally well-documented for its various medicinal benefits. This investigation is the first report on the potential antioxidant and antinfammatory reno-protective impact of GRV against DMBA-induced nephrotoxicity in rats.Methods: Forty male albino rats were allocated into four equal groups (n = 10). The 1st group served as the control, the 2nd group (GRV) was gastro-gavaged with GRV (200 mg/kg b.wt), the 3rd group (DMBA) was treated with a single dose of DMBA (15 mg/kg body weight), and the 4th group (DMBA + GRV) was gastro-gavaged with a single dose of DMBA, followed by GRV (200 mg/kg b.wt). The GRV administration was continued for 8 weeks.Results and Discussion: Results revealed a significant improvement in renal function, represented by a decrease in urea, creatinine, and uric acid (UA) in the DMBA + GRV group. The antioxidant potential of GRV was confirmed in the DMBA + GRV group by a significant decline in malondialdehyde (MDA) and a significant increase in catalase (CAT), superoxide dismutase (SOD), glutathione S transferase (GST), and reduced glutathione (GSH) compared to DMBA-intoxicated rats; however, it was not identical to the control. Additionally, the antiinflammatory role of GRV was suggested by a significant decline in mRNA expression of cytochrome P450, family 2, subfamily e, polypeptide 1 (CYP2E1), tumor necrosis factor-alpha (TNF-α), and interleukin 1 beta (IL-1β) in the DMBA + GRV group. Moreover, GRV improved the histopathologic and immunohistochemical expression of TNF-α, CYP450, and IL1β in DMBA-intoxicated kidney tissue. Conclusively, GRV is a natural medicinal product that can alleviate the renal injury resulting from environmental exposure to DMBA. The reno-protective effects of GRV may involve its anti-inflammatory and/or antioxidant properties, which are based on the presence of phytochemical compounds such as acetogenins, alkaloids, and flavonoids

Natural Ghee Enhances the Biochemical and Immunohistochemical Reproductive Performance of Female Rabbits

The reproductive effects of several dietary fats (margarine, ghee, and olive oil) on female rabbits were studied. For that purpose, 40 mature female rabbits were designed into four groups of ten rabbits each. Group I was given a control diet, Group II received 10% margarine, Group III received 10% ghee, and Group IV received 10% olive oil; after two months, all rabbits were sacrificed. Lipid profile and reproductive hormones levels were assayed in serum besides ovarian antioxidant enzyme and lipid peroxidation. Furthermore, ovarian tissue was examined using hematoxylin–eosin staining and immunohistochemistry of estrogen, follicle-stimulating hormone (FSH), luteinizing hormone (LH) receptor, and caspase 3. Our data revealed that the margarine significantly (p p p > 0.05) increase in the olive oil and ghee- treated group compared to the control group. At the same time, there was a significant increase in serum FSH and (estradiol (E2)) in the ghee and olive oil groups, respectively, compared to the control. The margarine feed group showed moderate immunoreaction of estrogen, FSH, LH receptor, and strong caspase 3, while ghee and olive oil showed strong immunoreaction of estrogen, FSH, LH receptor, and mild immunoreaction of caspase 3 in ovarian tissue. Photomicrograph of rabbit ovarian tissue showed vacuolation in small and growing follicles in the margarine group but appeared normal in ghee and the olive oil-treated group. In conclusion, based on these results, olive oil and ghee have a strong capability of enhancing lipid profile, antioxidant status, and female hormonal functions

The Effect of Supplementation of Enzyme on Laying and Reproductive Performance in Japanese Quail Hens Fed Nigella Seed Meal

The effect of dietary enzyme (Optizyme) on the performance of Japanese quail hens fed isocaloric and isonitrogenous diets containing 0, 10, 20 and 30% of Nigella seed meal (NSM) as a protein source was studied. A corn-soybean meal diet was used as a control diet. Eight groups of birds having 8 replicates consisting of 2 females and 1 male were used, and productive and reproductive traits and egg quality were measured. Hens fed 10% NSM produced significantly more eggs than those fed 0, 20 and 30% NSM diets. Shell thickness was also significantly higher in the 10% NSM group. Supplementation of enzyme significantly improved egg production and tended to improve shell thickness when a 20% NSM diet was fed, but not when 30% NSM was given. Furthermore, the enzyme significantly improved the fertility rate when fed a 20% NSM diet, but not in the groups fed a 30% NSM diet. From these observations, Japanese quail hens can be fed diet containing 10% NSM. Moreover, this level can be increased to 20% when supplemented with enzyme