Description of the cDNA sequences isolated from <i>H. incana</i>.

a<p>GeneBank accession number (<a href="http://www.ncbi.nlm.nih.gov/genbank/" target="_blank">http://www.ncbi.nlm.nih.gov/genbank/</a>).</p>b<p>AGI gene code (<a href="http://www.arabidopsis.org/" target="_blank">http://www.arabidopsis.org/</a>).</p

Effects of lead treatment on primary root length in wild-type and knockout mutant seedlings of <i>A. thaliana</i>.

<p>Seedlings were grown for 13 days on vertically orientated agar plates with or without 40 µM of Pb(NO₃)₂. (A) <i>Atmt2a</i> T-DNA mutant analysis. (B) <i>Athma4</i> T-DNA mutant analysis. All results are the average value (± SE) of 16 seedlings. The letters represent statistically homogenous subgroups using LSD post hoc test at a α = 0.01 significance level.</p

Lead distribution in different parts of <i>H. incana</i>.

<p>Lead contents are expressed as (A) mg Pb.g⁻¹ DW or (B) mg Pb.organ⁻¹. Seedlings were grown for 2 months in hydroponic conditions supplied weekly with fresh media containing 100 µM Pb(NO₃)₂. Roots R = roots at the rosette stage; Roots F = roots at the floral stage; R leaves = rosette leaves; S leaves = stem leaves. Data are the average (± SE) of three independent measurements.</p

Lead concentration in shoots of <i>H. incana</i> growing on different polluted soils.

<p>Seedlings were grown on sterile compost for 20 days and transferred for 2 months on 4 different soils containing various amounts of total lead (soil 1∶6972 mg.kg⁻¹ DW, soil 2∶18626 mg.kg⁻¹ DW, soil 3∶7531 mg.kg⁻¹ DW and soil 4∶1577 mg.kg⁻¹ DW; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061932#pone-0061932-t001" target="_blank">Table 1</a>). Data are the average (± SE) of three independent measurements.</p

Pb levels of the metalliferous mine soils in the plant cultivation.

<p>Pb-polluted soils were collected in different zones from abandoned metalliferous mines of Oued El Himer, south of Oujda city in eastern Morocco. Data are the average (± SE) of three independent measurements.</p

Kinetic effect of lead exposure on lead accumulation in (A) shoots and (B) roots of <i>H. incana</i>.

<p>Seedlings were grown on hydroponic system and lead treatments were done after 2 weeks by adding 50, 100 and 300 µM Pb(NO₃)₂ in fresh medium. Data are the average (± SE) of three independent measurements.</p

Lead accumulation in roots and shoots of wild-type and knockout mutant seedlings of <i>A. thaliana</i>.

<p>Seedlings were collected after 13 days of culture on agar plates with or without 40 µM of Pb(NO₃)₂. (A) <i>Atmt2a</i> T-DNA mutant analysis. (B) <i>Athma4</i> T-DNA mutant analysis. All results are the average value (± SE) of three independent replicates. For the roots and leaves separately, the letters represent statistically homogenous subgroups using LSD post hoc test at a α = 0.01 significance level.</p