Towards the Electrochemical Diagnostic of Influenza Virus: Development of Graphene-Au Hybrid Nanocomposite Modified Influenza Virus Biosensor Based on Neuraminidase Activity

International audienceAn effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10-8 U mL-1 and 10-1 U mL-1 was found with a relative standard deviation value of 3.23% (for 10-5 U mL-1 of N, n:3) and a limit of detection value of 10-8 U mL-1 N. The developed biosensor was applied for real influenza virus A (H9N2) detection and very successful results were obtained

Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity

International audienceNeuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor's working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscop

Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity

Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor’s working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscopy

Automated detection and tracking of phlebotominaes

International audienceThis paper presents a method for detecting and tracking phlebotominaes that are vectors for many important diseases. A method based on the Gaussian mixture model is used for Foreground/Background classification. Then, the mathematical morphology operations are used to refine theclassification results and eliminate areas that do not represent phlebotominaes. Thereafter, the Kalman filter is used for the prediction and stimation of their positions, thus the Hungarian algorithm is used for the assignment of the detected positions to tracks. The results can help us study the behavior of these insects, and to improve traps and solutions that limit their spread in case of infection. The proposed method is tested on a real phlebotominaes video sequences recorded at Pasteur institute of Tunisia, and the experimental results show the efficiency of the proposed algorithm to accurately detect the position and track phlebotominaes