Detection of FMDV in experimentally infected animals using real-time RT-PCR and RT-RPA.

<p>Dpi, days post infection;</p>1<p>infected with serotype A22 Iraq 24/64;</p>2<p>infected with O Bulgaria 2011; neg, negative.</p

FMDV RT-RPA primers and exo-probe sequences aligned with the consensus sequence of 100 FMDV 3D genes downloaded from the Genbank.

<p>(Geneious® 6.1.5, Biomatters Limited, New Zealand). Mismatches are indicated in bold and underlined. The consensus sequence represents nt 7847–7961 of FMDV sequence JF749843. NNN are sites of the quencher and fluropohore in following order (BHQ1-dT) (Tetrahydrofuran) (FAM-dT). Y is C & T; R: A & G.</p

Performance and analytical sensitivity of the FMDV RT-RPA assay.

<p>A: Semi-logarithmic regression of the data collected from eight FMDV RT-RPA test runs on the RNA standard using Prism Software. It yielded results between 4–10 minutes. B: Probit regression analysis using Statistica software on data of the eight runs. The limit of detection at 95% probability (1436 RNA molecules) is depicted by a triangle.</p

Comparison between real-time RT-RPA and RT-PCR for the detection of FMDV in clinical samples During Egypt 2012 FMD outbreak.

<p>Forty-five RNA extracts of samples collected from suspected cases of FMDV were screened. Linear regression analysis of RT-RPA threshold time (Y axis) and RT-PCR cycle threshold values (X axis) were determined by Prism software. R squared value was 0.26.</p

FMDV RT-RPA.

<p>Fluorescence development over time using a dilution range of 10⁷-10¹ molecules/µl of the FMDV RNA standard (Graph generated by ESEquant tubescanner software). F04+R20+P2 were employed and the analytical sensitivity was 10². 10⁷ represented by black line; 10⁶, gray; 10⁵, red; 10⁴, blue; 10³, green; 10², cyan; 10¹, dark khaki; negative control, orange.</p

Phenotypic features of patients with Schimke immuno-osseous dysplasia (n = 34).

<p>Phenotypic features of patients with Schimke immuno-osseous dysplasia (n = 34).</p

Localization of <i>SMARCAL1</i> mutations detected in 34 subjects with Schimke immuno-osseous dysplasia.

<p>Legend: : recurrent mutations detected in at least three unrelated patients; Green font: novel mutations described for the first time in the current study. Reference sequence: ENST00000357276; NM_014140.</p