Kinetics of pro-inflammatory proteins synthesized by infected Hbmec.

<p>A and B: cytokine expression profiles; C and D: chemokine expression profiles; E and F: growth factor and SERPIN E1 expression profiles were determined after 8 h, 14 h, 24 h and 48 h infection by two strains of <i>T. gondii</i> RH (Figure 1A, 1C and 1E) and PRU (Figure 1B, 1D and 1F). A basal expression level for each pro-inflammatory protein <i>T. gondii</i> burden was determined at T₀. Concentrations of immune mediators were calculated using values of corrected pixels obtained from uninfected cells (negative control) subtracted from pixel values of infected cells. <i>T. gondii</i> burdens were evaluated by the percentage of <i>Toxoplasma</i> multiplication, knowing that the parasite burden at T₀ is 100%. The parasite burden scale was multiplied 100 times (Only in Hbmec infected by RH strain). *<i>p</i><0.05, ** <i>p</i><0.01 and *** <i>p</i><0.001.</p

Kinetics of pro-inflammatory proteins synthesized by infected CMH5.

<p>A and B: cytokine expression profiles; C and D: chemokine expression profiles; E and F: growth factor and SERPIN E1 expression profiles were determined after 8 h, 14 h, 24 h and 48 h infection by two strains of <i>T. gondii</i> RH (Figure 2A, 2C and 2E) and PRU (Figure 2B, 2D and 2F). A basal expression level for each pro-inflammatory protein <i>T. gondii</i> burden was determined at T₀. Concentrations of immune mediators were calculated using values of corrected pixels obtained from uninfected cells (negative control) subtracted from pixel values of infected cells. <i>T. gondii</i> burdens were evaluated by the percentage of <i>Toxoplasma</i> multiplication, knowing that the parasite burden at T₀ is 100%. *<i>p</i><0.05, ** <i>p</i><0.01 and *** <i>p</i><0.001.</p